new test-kits to detect herbicide effects and resistance ...€¦ · biotest-kit (hwr-test) new...
TRANSCRIPT
![Page 1: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/1.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
New test-kits to detect herbicide effects and
resistance in weed plants based on HPTLC-screening
Dr. Helle Weber RavnUniversity of Aarhus
National Environmental Research InstitutePhytoChemLab
![Page 2: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/2.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Overview of presentation:• PhytoChemLab• BioTest-kits & ChemiTest-kits• International patent applications• What, why and who?• Claims• Biomarker pattern • Weed plants/herbicides • CAMAG HPTLC-equipment• HWR-Test® (BioTest-Kit)• Semi-/field validation• Horizontal/vertical development • HR-Test (BioTest-kit)• Collaborators• Summary
![Page 3: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/3.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
PhytoChemLabA Plant Biomarker Laboratory with
innovative perspectives
Dr. Helle Weber Ravn (Head of Laboratory) Lise Lauridsen & Anni Christiansen
• Research: Biomarkers in biological organisms after exposure to chemicals
• Development of simple tools as test-kits to be used in the field for effect studies
• Supervision of master and Ph.D-students
• International patent applications
• Close collaboration with private companies
![Page 4: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/4.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Bio- and ChemiTest-kits
New tool to farmers for early and fast detection of
herbicide resistance in weed plants
BioTest-kits (HR-Test Kit)
A tool to farmers for early and fast detection of
herbicide effects in weed plants
BioTest-kit (HWR-Test)
New tools to detect chemicals/nano-particles in
the environment
ChemiTest-kits
![Page 5: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/5.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
International patent applications• International PCT-US patent application:
An assay method and kit for testing biological material for exposure to stress using biomarkers (WO 01/92879 A1 PCT/DK01/00377)Application date: 30 May 2000Inventor: Helle Weber RavnRights: National Environmental Research Institute
• International PCT-Patent application:A method and kit for testing stress effects in living organism (PA 2007 00022)Application date: 7 January 2007Inventor: Helle Weber RavnRights: National Environmental Research Institute & The Science and Agricultural Science Faculty, Aarhus University (3:2)
• International PCT-Patent application:Method and kit for detecting resistance in living organism (PA 2007 00024)Application date: 7 January 2007; Inventors: Helle Weber Ravn : Per Kudsk Nielsen : Solvejg K. Mathiassen (2:1:1)Rights: National Environmental Research Institute Institute & The Science and Agricultural Science Faculty, Aarhus University (1:1)
![Page 6: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/6.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
What can we do?• A cheap, fast and easy tool to
control the effect of a given herbicide dose only a week after spraying and before visual signs (BioTest-kit: HWR-Test®)
• Cheap, fast and easy tools to detect herbicide resistance in weed plants short time after spraying (BioTest-kit: HR-Test)
![Page 7: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/7.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Who can use the tools?
•Farmers•Consultants
![Page 8: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/8.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Why do we want that?• The farmer will save time and money if
no further spraying is needed (e.g. rain shortly after spraying) (HWR-Test®)
• The farmer can optimize the use of e.g. reduced doses of herbicides, protection of the Environment (HWR-Test®)
• The farmer can detect herbicide resistance and use other pesticides(HR-Test)
![Page 9: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/9.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
What is the claims to test-kits?
• Reliability• Easy to use• Fast results• Cheap to use• Low detection limit (ChemiTest-
kits)
![Page 10: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/10.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Principle BioTest-Kits:• to use the early biochemical responses in
plants short time after herbicide spraying
• to use the differences in the plants chemical composition and concentration (biomarkers) in relation to the wanted effect
ChemiTest-Kits:• to detect the concentration of
chemicals/nanoparticles in different biological media and the environment
![Page 11: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/11.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Biomarkers in BioTest-KitsBiomarkers (invisible)
Biochemical changes
Physiological changes
Visual effects (morphological changes)
![Page 12: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/12.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
BioTest-kit (HWR-Test) (effect):Weed plants & herbicides
Species• Loose silky bent (Ápera spica-vénti )• Perennial rye grass (Lolium perénne)• Annual meadow grass (Poa ánnua)
Herbicides• Iodosulfuron (Hussar OD, Bayer CropScience)• Mesosulfuron + iodosulfuron (Atlantis WG,
Bayer CropScience)
![Page 13: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/13.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Biomarkers
Control/Herbicide exposed plant extracts
Two differentgroups of
compounds
![Page 14: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/14.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Equipment for method development
Camag Applicator, Video-Store & Scanner (Supported by the National Research Council, Denmark)
![Page 15: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/15.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
BioTest-kit development
![Page 16: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/16.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
HWR-Test® SHerbicide Weed Response Test
to Sulfonylurea-herbicides
www.HWR-Test.com
![Page 17: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/17.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
HWR-Test® S 3 steps
1) Sample preparation
2) Extraction
3)Filtration4) Chemical reactionand detection
![Page 18: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/18.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
HWR-Test® Semi-field tests
Correspondence between plant biomass reduction and colour reaction of phytochemicalbiomarkers
N=recommended doses
Control 1/32N 1/16N 1/8N 1/4N 1/2N 1N
Response of weed plants to herbicide treatment
![Page 19: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/19.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn HWR-Test®
Semi-field study on Lolium perenneexposed to Hussar (iodosulfuron) –
Dose/Responses
4 days after exposure
0
1000
2000
3000
4000
5000
0 N 1/32 N 1/16 N 1/8 N 1/4 N 1/2 N 1/1 N
Herbicide doses
Hig
h o
f colo
ur
inte
nsit
y
(AU
)
0
20
40
60
80
100
% R
edu
cere
d g
row
th2
1 d
ays
aft
er
spra
yin
g
HWR-Test®
Semi-field study on Lolium perenne exposed to Hussar (iodosulfuron) – Dose/Responses
Red zone
Yellow zone
Green zone
![Page 20: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/20.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Field-tests (DJF/NERI) Biomarker/Effect studies af BioTest-Kits
![Page 21: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/21.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Results of field validationPoa annua - Atlantis - 7 days after
exposure
0500
100015002000250030003500400045005000
0 50 100 200
g/Ha
Hig
ht
af
inte
nsit
y
(AU
)
Lolium perenne - Atlantis - 7 days after exposure
0
1000
2000
3000
4000
5000
0 50 100 200
g/Ha
Hig
ht o
f in
tens
ity (A
U)
0N ¼ N ½N 1N
Red Yellow green zones
Differences in sensitivities
![Page 22: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/22.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Horisontal development of BioTest-kit 1 (effect):Weed plants & herbicides
Species• Common chickweed (Stellaria media) • Wall speedwell (Veronica arvensis) • Common poppy (Papaver rhoeas)• Field pansy (Viola tricolor) • Loose silky bent (Ápera spica-vénti )• Black-grass (Alopecurus myosuroides)
Herbicides• Express ST (tribenuron-methyl)• Monitor (sulfosulfuron)• Safari (triflusulfuron-methyl)• Ally ST(metsulfuron-methyl)• Harmony Plus (thifensulfuron-methyl + tribenuron-methyl)• MaisTer (foramsulfuron + iodosulfuron)• Titus WSB (rimsulfuron)
![Page 23: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/23.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Vertical development1. Step
Development of simple field technological equipment –collaboration with HIH-AU, Herning 3th semester 6 engineer students in autumn 2008 at NERI (financed by Frisenette ApS, Knebel
2. Step
A business Ph.D. (if financial support can be identified) at PhytoChemLab
![Page 24: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/24.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Herbicide Resistance Test –HR-Test
GoalA new simple and fast tool to detect herbicide resistance in weed plants after exposure
Coordinator: Dr. Helle Weber Ravn, NERI
Collaboration project between Aarhus Universityand a private company: Frisenette ApS
![Page 25: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/25.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Coordinators and Collaborators
National Environmental Research Institute, University of Aarhus, Silkeborg
Dr. Helle Weber Ravn (project leader), Lise Lauridsen & Anni Christiansen
Danish Faculty of Agricultural Science, University of Aarhus Flakkebjerg
Mr. Per Kudsk & Dr. Solvejg K. Mathiassen
Hobro - Aalborg Union of Farmers, HobroMrs. Hanne K. Kristensen
Frisenette ApS, KnebelErvind Müller & Søren H. Christensen (product development & forhandler)
Faculty of Pharmacy, University of Montpellier I, FranceDr. Laurence Mondolot (Histochemical investigations)
![Page 26: New test-kits to detect herbicide effects and resistance ...€¦ · BioTest-kit (HWR-Test) New tools to detect chemicals/nano-particles in the environment ChemiTest-kits. University](https://reader031.vdocuments.us/reader031/viewer/2022011913/5fa8159e2505133677064317/html5/thumbnails/26.jpg)
University of Aarhus - National Environmental Research Institute - Department of Terrestrial Ecology - PhytoChemLab
Pla
nt
Bio
mark
ers
-D
r. H
ell
eW
eb
er
Ravn
Summary:• A cheap, fast and easy tool to control
the effect of a given herbicide dose only a week after spraying and before visual signs (HWR-Test®) is developed
• Cheap, fast and easy tools to detect herbicide resistance in weed plants short time after spraying (HR-Test) is in development
• Other simple test-kits can be developed