new generation tissue homogenizer sample preparation ... brochure.pdf · new generation tissue...
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![Page 1: NEW GENERATION Tissue homogenizer SAMPLE PREPARATION ... Brochure.pdf · NEW GENERATION TISSUE HOMOGENIZER Sample Prep and Cell Lysis Precellys is your solution for sample preparation](https://reader036.vdocuments.us/reader036/viewer/2022062607/6043c150560f082e764d1c04/html5/thumbnails/1.jpg)
APPLICATIONS
R&D BiotechnologyMolecular biology and proteomic
Genotype determination
Pharmacology and ToxicologyToxic analysis, Metabolism studies
Forensic MedicineDNA/RNA tests, Drugs and toxics detection
Food & EnvironmentGMO detection, Hygiene controls
Materials research
© B
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DISTRIBUTOR
[email protected] : +33(0)139 306 118Fax : +33(0)139 306 185
VISIT OUR PROTOCOLS DATABASE
Take a look to scientifi c publications and other application formson our website :www.precellys.com
CONTACT
BERTIN TECHNOLOGIESParc d’Activités du Pas du Lac10, bis Avenue Ampère - BP28478180 Montigny-le-BretonneuxFRANCE
SAMPLE PREPARATION & CELL LYSIS
www.precellys.com
NEW GENERATION Tissue homogenizer
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NEW GENERATION TISSUE HOMOGENIZER Sample Prep and Cell Lysis
Precellys is your solution for sample preparation and cell lysis
The reliable, fast and high-throughput homogenizer to extract DNA, RNA or proteins from animal, human and plant tissues, micro-organisms, spores, bones, etc.
FAST - Homogenize up to 24 samples in few seconds
EFFICIENT - Retain integrity of any biological molecule DNA, RNA, protein, drug, etc.
FLEXIBLE - Adapt protocols and consumables from soft to hard tissues (micro-organisms, animal or vegetal tissues)
CROSS CONTAMINATION FREE - 2mL single-use tubes
REPRODUCTIBLE - Standard protocols
HIGH-QUALITY - Robust, ergonomic and programmable
Precellys®24
PRECELLYS®24 : Bead beating homogenizer
Chromatograms of liquid / liquid extraction from animal tissues.
DNA isolation from plant tissues.
Western Blot from proteins in micro-organisms.
Precellys kitReady to use 2mL tubes fi lled with beads
ANIMAL & HUMAN TISSUES
1. Load from 1 to 24 tubes 2. Patented system for quick and safe blocking
4. Bead beating motion, homogenization is achieved in few seconds
3. Set speed, time, cycle and break.
PLANT TISSUES
MICRO-ORGANISMS
Grind and homogenize a large range of biological samples, compatible with any analysis DNA-RNA Electrophoresis, Liquid Chromatography, Western Blot etc.
5. Ready for analysis
DNARNADrugsProteins
Developed to be used in L3 Laboratory
L : 290 mmW : 375 mmH : 370 mmH of opened cover : 600 mm
Weight : 29,8 kg
Universal Power :100 - 230 VAC / 50 - 60 z
VKOI & VKO5 Small Glass Beads
Special design for micro-organism lysis. Smooth impacts break the
membrane of cells,bacterias, spores, etc.
CK14 Small Ceramic Beads
Performs perfect homogenization of any soft animal, human and plant
tissues : Brain, liver, leaf, etc.
MK28 Big Metal Beads
Gives the ultimate strength to grind challenging samples of
animal, human and plant tissues : Hair, bones, corn, etc.
CK28 Big Ceramic Beads
Offers more strength for the homogenization of harder animal,
human and plant tissues :Heart, nails, nuts, etc.
6. Technical Specifi cations
Example :
Example :
Example :
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� Duality: 7 mL & 2 mL tubes
� Flexibility: from 1 mg to 5 g
� Efficiency: High yield and integrity for
Extend your Precellys®24 experience
with 7 mL lysing tubes. Handle all sample preparations with the same equipment, not only from soft to hard tissues but also from 1mg to 5000 mg. Maximize your results and save time!
7 mL 2 mL
Precellys®24-Dual tissue homogenizer allows the simultaneous homogenization of six 7 mL lysing tubes or
twelve 2 mL lysing tubes.
Discover the Discover the Discover the Discover the newnewnewnew PrecellysPrecellysPrecellysPrecellys®24242424----DualDualDualDualThe only efficient large volume tissue homogenizer
Now you can choose
7 mL Precellys Lysing Kits
Dual Dual Dual Dual Actual size
DNA, RNA, proteins…
Cat No.0904-01 Cat No.0907-04 Cat No.0907-06 Cat No.0907-052.8 mm ceramic beads
Hard tissue homogenizing
CK28
Glass and ceramic beads
Soil grinding
0.1mm ceramic beads
Micro-organism lysingGlass mix beads
Micro-organism lysing
Soil Mix CK01 VK01/VK05
To optimize your grinding efficiency add beads of 5.0mm ceramic beads(03961-1-106) or 6.8mm ceramic beads(03961-1-107)
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Combine yield and integrity of thermo sensible molecules with the association of Cryolys cooling option and Precellys®24-Dual.Precellys® 24 system is the only cooled equipment using bead beating in large volume.
CONTACT US
AsiaElina [email protected]
+852 9860 6075
AmericaCaroline [email protected]+1 (302) 351 4429
EuropeRomain [email protected]+33 139 306 118
Cool your samples with Cryolys
Biological applications – 7 mL vials37
12-8
10-D
U02
7 / N
ovem
ber
2009
Drug extraction6000 rpm - 1x20s4 ml Buffer0904-011 gMuscle, Kidney,
Liver (Pork)
Fine powdering6000 rpm - 2x30sno buffer0904-012 tablets (0,86g)
Yeast Brewer's tablets
DNA extraction5000 rpm - 3x15s4 ml Buffer0907-04 + 2 beads
03961-1-1061 g
Microorganisms from Soil
RNA extraction6500 rpm - 2x20s5 ml Trizol0907-06500 mgLactic bacteria
from curd
Microorganism
s
Drug extraction5500 rpm - 2x10s4 ml Acetonitril70%0904-011 gWhole brain (rat)
Drug extraction6500 rpm - 2x20s*3 ml Acetonitril50%0904-01
1,2 - 1,4 gWhole lung (rat)
RNA extraction6000 rpm - 2x15s2,4 ml Buffer
0904-011 gHeart Anim
als tissues
Enzymatic activity6000 rpm - 2x60s*1 ml Buffer0904-01 + 1 bead
03961-1-1071 seedDurum wheat
DNA extraction6000 rpm - 2x30sno buffer0904-01 + 2 beads
03961-1-1071 seedCorn grain
Virus detection6500 rpm - 2x20sno buffer0904-01 + 2 beads 03961-1-106 + 2
beads 03961-1-107
2 g (250 seeds)Seeds tomato
RNA extraction5500 rpm - 2x15s1 ml Buffer0904-011 gLeaves Thyme
RNA extraction5500 rpm - 2x15s1 ml Buffer0904-011 gLeaves basilica
Plant tissues (leaves, seeds)
AnalysisPrecellys protocolsBuffer
Precellys lysingkitWeightSamples
* With Cryolys cooling option.
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MicroTubes prefilled with beads0.5mL - 2mL - 7mL
Precellys®24-DualTissue Homogenizer
Precellys lysing kits
Precellys lysing kits 2010
Choose the Precellys lysing kit for your samples!
LYSE ! HOMOGENIZE ! GRIND !
VK01 VK05 VK01-05 CK01 CK14 CK28 CKMix MK28 Soil Mix
human cells ���� ���� ���� ����
gram+ ���� ���� ���� ����
gram- ���� ���� ���� ����
bacteria ���� ���� ���� ����
yeast ���� ���� ����
fungi ���� ���� ����
MICRO BIAL
spores ���� ���� ����
brain ���� ����
liver ���� ����
kidney ���� ����
ear ���� ���� ����
lung ���� ���� ����
heart ���� ���� ����
spleen ���� ���� ����
tail ���� ����
cornea ���� ����
artery ���� ����
colon ���� ����
intestine ���� ����
pancreas ���� ����
muscle ���� ���� ����
skin ���� ���� ����
spincor ����
bone ����
teeth ����
ANIMAL
hair ����
leaves ���� ���� ����
arabidopsis ���� ���� ����
seeds ���� ���� ����
corn ���� ���� ����
nuts ���� ���� ����
rice ���� ����
PLANT
wheat ���� ����
soil ����
fecal ���� SOIL sediment ����
CAT.NO. DESCRIPTION TUBES PACK
03961-1-001 Hard tissue grinding MK28 2mL STD 50 preps
03961-1-002 Hard tissue homogenizing CK28 2mL STD 50 preps
03961-1-003 Soft tissue homogenizing CK14 2mL STD 50 preps
03961-1-203 Soft tissue homogenizing CK14-0,5mL 0,5mL STD 50 preps
03961-1-004 Tough micro-organism lysing VK05 2mL STD 50 preps
03961-1-204 Tough micro-organism lysing VK05-0.5mL 0,5mL STD 50 preps
03961-1-005 Micro-organism lysing VK01 2mL STD 50 preps
03961-1-006 Soil grinding SK38 2mL STD 50 preps
03961-1-007 Hard tissue homogenizing CK28-R 2mL-R 50 preps
03961-1-008 Hard tissue grinding MK28-R 2mL-R 50 preps
03961-1-009 Tissue homogenizing CKMix 2mL STD 50 preps
0904-01 Tissue homogenizing CK28 7mL 50 preps
0907-04 Soil grinding CK14/VK01 7mL 50 preps
0907-05 Micro-organism lysing VK01/VK05 7mL 50 preps
0907-06 Micro-organism lysing CK01 7mL 50 preps
03961-1-106 5.0mm Zirconium oxide beads - Beads in bulk
03961-1-107 6.8 mm Zirconium oxide beads - Beads in bulk
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LYSE !HOMOGENIZE ! GRIND !
Cat. No. 03961-1-005 (2mL tubes)
Cat. No.03961-1-204 (0,5mL tubes)Cat. No. 03961-1-004 (2mL tubes)
Application Example:- Proteins extraction from Mycobacterium Smegmatis
Cat. No. 03961-1-203 (0,5mL tubes)Cat. No. 03961-1-003 (2mL tubes)
Application Examples:- Prion extraction for ESB- RNA extraction for mouse liver
CK14
Cat. No. 03961-1-002 (2mL tubes)Cat. No. 03961-1-007 (2mL reinforced tubes) Cat. No. 0904-01 (7mL tubes)
Application Examples:- Proteins extraction from mouse heart- RNA extraction from human skin
MK28
Cat. No. 03961-1-001 (2mL tubes)Cat. No. 03961-1-008 (2mL reinforced tubes)
Application Examples:- DNA extraction from rice Grain for GMO determination- Pulverization of Hair for drugs analysis
Cat. No. 03961-1-006 (2mL tubes)Cat No. 0907-04 (7mL tubes)
Application Example: - DNA/RNA extraction from environmental samples
Cat. No. 03961-1-009 (2mL tubes)
Application Example:- DNA extraction from leaf
VK05
Big beads for 7 mLnew
Cat. No. 0907-05 (7mL tubes)
Application Example:- Recombinant proteins extraction from E.Coli
Cat. No. 0907-06 (7mL tubes)
Application Example:- RNA extraction from Lactic bacteria from curdApplication Examples:
- Virus detection from tomato seedsAdd 2 beads of 03961-1-106 and 2 beads of 03961-1-107 in the 7mL tubes 0904-01- DNA extraction from Microorganisms from soilAdd 2 beads of 03961-1-106 in the 7mL tubes of 0907-04
Cat. No. 03961-1-106 = 5.0mm Zirconium oxide beadsCat. No. 03961-1-107 = 6.8mm Zirconium oxide beads
Add beads in your 7 mL lysing tubes to optimize your grinding efficiency
VK01 - VK05new
new
1.4 mm ceramic beads
2.8 mm ceramic beads
Ceramic mix beads
2.8 mm metal beads
Glass and ceramic mix beads
0.5 mm glass beads
- VK01 = 0.1 mm glass beads
- VK01 / VK05 = glass mix beads
0.1 mm ceramic beads
CK28
CKMix
Soil Mixnew
CK01
Login and find application protocolswww.precellys.com – Application CenterFor customer suppory, email [email protected]
Hard tissue grindingSoft tissue homogenizing
Tissue homogenizing
Hard tissue homogenizing Soil grinding
Micro-organism lysing
Micro-organism lysing
50 prepsReady-to-use
Quantity discount available
0.5mL 2mL
2mL 2mL-R7mL
2mL
2mL 2mL-R
2mL7mL
2mL
0.5mL 2mL
7mL
7mL
Tough micro-organism lysing
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