nerc chem hyg plan micro

7/31/2019 Nerc Chem Hyg Plan Micro

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Acknowledgements

Parts of this policy were written by the Chemistry Department, Rick Sobel, and some items may have been

learned through the many faculty and staff members who have traveled through the laboratory over the years. Other

parts, written by myself, Ron Miraglia, did not come without the experience of working together with both Rick Sobel

and Ardemiss Ayanian. Further additions to this policy came from the pooled experience of the three of us as we sat

and conveyed ideas about different issues and subjects and resolved various problems and situations without getting

into heated arguments (I think?).

Also, it does not come without admitting the contributions of the many laboratory assistants who have

transited the laboratory over the years. These students either led or helped us in putting these rules and procedures

together in one way or another.

Additionally, I could not have compiled this policy without the assistance of Frank Perri. Frank devoted

many hours to keying in the data, formatting scanned data, and continually adjusting text and graphics. He also

structured some of the information, made corrections and added statements to the text.

In retrospect, it has taken many years of experience and the contributions of a good number of individuals to

put this policy together. And, in the future, we will continue to review it and to make changes as necessary to remain

compliant with OSHA regulations.

Parts of this policy have been rewritten by Pat Hollabaugh and Laura Grussendorf, Laboratory

Managers/Assistants at the Northeast Regional Center, so the policy will be specific to that particular Regional Center.


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TABLE OF CONTENTS

DEPARTMENT STATEMENT. ................................................................................. 6ANNUAL REVIEW. ...6DISCLAIMER.. ..6

THE OSHA LABORATORY STANDARDINTRODUCTION .................................................................................................................................. 7

EMPLOYEE RIGHTS AND RESPONSIBILITIES ................................................................. 7

HAZARDOUS CHEMICALS ................................................................................................... 8

MATERIAL SAFETY DATA SHEETS (MSDS) ..................................................................... 9

CHEMICAL INVENTORIES ................................................................................................... 9

THE CHEMICAL HYGIENE PLAN

INTRODUCTION ................................................................................................................................ 10

SCOPE AND APPLICATION ................................................................................................ 10

RESPONSIBILITY.................................................................................................................. 10EXPOSURE LIMITS ............................................................................................................... 11

WORK RELATED INJURIES ................................................................................................ 11

PENNSYLVANIA EYE PROTECTION ACT......... 12

PART I: DEPARTMENT, FACULTY & STAFF.............................................................................13

DEPARTMENT INTRODUCTION...................................................................................................13

SAFETY INFORMATION ................ 14

LABELS AND HAZARDOUS IDENTIFICATION

MSDS SHEETS AND REFERENCE MATERIALS

PERSONAL PROTECTIVE EQUIPMENT (PPE)

EYEWASHESSAFETY SHOWER

FIRE BLANKET

FIRE EXTINGUISHERS

EMERGENCY CUT-OFF LEVERS

EMERGENCY LIGHTING

ELECTRICAL BOXES

SPILL CLEAN-UP

FIRE DRILL & EVACUATION

LABORATORY INFORMATION........ (North, East Buildings)..........................................................18

LABORATORY INTRODUCTION

PREP ROOM

VENTILATION HOODSGLASSWARE & EQUIPMENT

STORAGE ROOM

WASTE DISPOSAL

HOUSEKEEPING

LABORATORY ROOM SET-UP

SEMESTER BREAK

MAINTENANCE LIST


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LABORATORY WORKERS 22INTRODUCTION TO TRAINING

LABORATORY INFORMATION

HIRING POLICY

LABORATORY WORKERS SAFETY TRAINING ..................................................................... .27LABELS AND HAZARDS

MSDSPPE

SAFETY GLASSES

LAB COATSGLOVES

FACE MASKS & RESPIRATOR

EYE WASHES

SAFETY SHOWER

FIRE BLANKET

FIRE EXTINGUISHER

EMERGENCY CUT-OFF

LABORATORY VENTILATION HOODS

EMERGENCY LIGHTINGELECTRICAL BREAKER BOXES

CHEMICAL & EQUIPMENT STORAGE

PREP ROOM & STORAGE

GLASSWARE & EQUIPMENT

HOUSEKEEPING

LABORATORY WORKERS HANDS-ON TRAINING.35ORIENTATION TO THE LABORATORY AND TRAINING

LABORATORY ROOM GRAPHICS AND EQUIPMENT LIST

LABORATORY ROOM SCHEMATIC

PREP ROOM SCHEMATICS

HANDS-ON TRAINING

USING THE BALANCECHEMICAL PREPARATIONS

EQUIPMENT DEMO SET-UPS

PREPARING SOLUTIONS

DILUTING ACIDS AND BASES

EQUIPMENT DEMO SET-UPS

EXPERIMENT PREPARATION

ROOM PREPARATION

CERTIFICATION PAGE

PART II: LABORATORY POLICIES AND PROCEDURES ...................................................... 50

NEW INSTRUCTORS POLICY

LABORATORY CHECK-IN POLICYSAFETY TEST POLICY

INCIDENT REPORT

SPILL CLEAN-UP PROCEDURE

LABORATORY ROOM SHUT-DOWN PROCEDURE

CO-OP POLICY

INTER-SEMESTER BREAK


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APPENDICESA. MSDS INFORMATIONB. CHEMICAL INVENTORYC. RECORD-KEEPING LOGD. PENNSYLVANIA EYE PROTECTION ACTE. AMERICAN NATIONAL STANDARDS INSTITUTE (ANSI)

F. FIRE EXTINGUISHER INFORMATIONG. ACID & BASE CLEAN-UP INSTRUCTIONSH. GENERAL LABORATORY SAFETY RULESI. RESOURCES


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DEPARTMENT STATEMENT

This document was developed for the NERC Laboratories to meet the compliance standards

required by the Occupational Safety and Health Administration (OSHA) regulations 29 CFR

1910.1450 and 29 CFR 1910/1200 C. These regulations require laboratories that use

hazardous chemicals to protect employees by implementing a Chemical Hygiene Plan

(CHP).

The Department will provide appropriate safety information and laboratory policies and

procedures to new employees through the Chemical Hygiene Plan. This Chemical Hygiene

Plan covers all common safety and laboratory health related issues. In general terms, this

document is comprehensive in that it covers safety practices and procedures of the NERC

laboratories of Community College of Philadelphia. However, any group-specific

procedures that pose a health risk should be posted with this document by the individual

faculty or groups involved.

This Chemical Hygiene Plan will be posted in the central part of the Prep Room of Main

Campus (W4-50), Northeast Regional Center (NERC) and the Northwest Regional Center

(NWRC). It is required that all employees read this document.

On the following pages is a more detailed description of what the OSHA Laboratory

Standard and Chemical Hygiene Plan covers.

ANNUAL REVIEW

The laboratory supervisors will perform an annual review of the Chemical Hygiene Plan to ensure that it

remains comprehensive. Any additions or changes will be made after careful review of any new materials

or suggestions that arise. The annual review will take place each year in December.

DISCLAIMER

The materials contained in this manual have been compiled by members of the Department of Chemistry

of the Community College of Philadelphia from sources believed to be reliable and to represent the best

opinions on the subject. This manual is intended to serve only as a starting point for good practices and

does not intend to specify minimal legal standards or to represent any other policies. No warranty,

guarantee, or representation is made by the Department of Chemistry as to the accuracy of sufficiency of

the information contained herein, and the department assumes no responsibility in connection therewith.Therefore, it cannot be assumed that all necessary warning and precautionary measures are contained in

this document and that other or additional information or measures may not be required. Users of this

manual should consult pertinent local, state and federal laws and legal counsel prior to initiating any

accident-prevention program.


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THE OSHA LABORATORY STANDARD

INTRODUCTION

The basis for this standard (29 CFR 1910.1450) is a determination by the Occupational Safety and Health

Administration (OSHA), after careful review of the complete rule-making record, that laboratories

typically differ from industrial operations in their use and handling of hazardous chemicals and that a

different approach than that found in OSHA's substance specific health standards is warranted to protect

workers. The final standard applies to all laboratories that use hazardous chemicals in accordance with the

definitions of laboratory use and laboratory scale provided in the standard. Generally, where this standard

applies it supersedes the provisions of all other standards in 29 CFR, part 1910, subpart Z, except in

specific instances identified by this standard. For laboratories covered by this standard, the obligation to

maintain employee exposures at or below the permissible exposure limits (PELs) specified in 29 CFR,

part 1910, subpart Z is retained. However, the manner in which this obligation is achieved will be

determined by each employer through the formulation and implementation of a Chemical Hygiene Plan

(CHP). The CHP must include the necessary work practices, procedures and policies to ensure that

employees are protected from all potentially hazardous chemicals used or stored in their work area.

Hazardous chemicals as defined by the final standard include not only chemicals regulated in 29 CFR part

1910, subpart Z, but also any chemical meeting the definition of hazardous chemical with respect to

health hazards as defined in OSHA's Hazard Communication Standard, 29 CFR 1910.1200(c).

Among other requirements, the final standard provides for employee training and information, medical

consultation and examination, hazard identification, respirator use and record keeping. To the extent

possible, the standard allows a large measure of flexibility in compliance methods.

Effective Date: May 1, 1990. Compliance Date: Employers shall have completed an appropriate

Chemical Hygiene Plan and commenced carrying out its provisions by January 31, 1991.

EMPLOYEE RIGHTS AND RESPONSIBILITIES

Employees have the right to be informed about the known physical and health hazards of the chemical

substances in their work areas and to be properly trained to work safely with these substances.

Employees have the right to file a complaint with OSHA if they feel they are being exposed to unsafe or

unhealthy work conditions. Employees cannot be discharged, suspended, or otherwise discriminated

against by their employer because of filing a complaint, or exercising their rights under the law.


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Employees have the responsibility to attend training seminars on the Laboratory Standard and Chemical

Hygiene Plan and to stay informed about the chemicals used in their work areas. They have the

responsibility to use safe work practices and protective equipment required for safe performance of their

job. Finally they have the responsibility to inform their supervisors of accidents and conditions or work

practices they believe to be a hazard to their health or to the health of others.

HAZARDOUS CHEMICALS

The OSHA Laboratory Standard defines a hazardous chemical as any element, chemical compound, or

mixture of elements and/or compounds which is a physical or health hazard.

A chemical is a physical hazard if there is scientifically valid evidence that it is flammable, a

combustible liquid, a compressed gas, an explosive, an organic peroxide, an oxidizer, pyrophoric,

unstable material (reactive), or water-reactive.

A chemical is a health hazard if there is statistically significant evidence based on at least one study

conducted in accordance with established scientific principles that acute or chronic health effects may

occur in exposed employees. Included are:

carcinogens irritants

reproductive toxins corrosives

sensitizers radioactive materials

neurotoxins (nerve) biohazards

hepatotoxins (liver) nephrotoxins (kidney)

agents that act on thehematopoietic system (blood)

agents that damage the lungs, skin,eyes, or mucous membranes

In most cases, the label will indicate if the chemical is hazardous. Employees should look for key words

like caution, hazardous, toxic, dangerous, corrosive, irritant, carcinogen, etc. Old containers of

hazardous chemicals (before 1985) may not contain hazard warnings.

An employee should review the Material Safety Data Sheet (MSDS) or contact their supervisor if they

are not sure whether or not a chemical they are using is hazardous.

Designated areas must be established and posted for work with certain chemicals and mixtures which

include select carcinogens, reproductive toxins, and/or substances which have a high degree of acute

toxicity. A designated area may be the entire laboratory,an area of a laboratory oradevice such as a

laboratory hood. Designated area postings and locations are available from your supervisor.


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MATERIAL SAFETY DATA SHEETS (MSDS)

A Material Safety Data Sheet (MSDS) is a document containing chemical hazard and safe handling

information prepared in accordance with the OSHA Hazard Communication Standard.

Chemical manufacturers and distributors must provide a MSDS the first time a hazardous

chemical/product is shipped to a facility.

Only MSDSs received must be retained and made available to laboratory workers. However, you can

request an MSDS for any laboratory chemical from the manufacturer or distributor.

If you want to review an MSDS, contact your supervisor or instructor. If you need an MSDS for your

work area file, contact the chemical supplier. See Appendix A for a more detailed description of the

MSDS sheets.

CHEMICAL INVENTORIES

The OSHA Laboratory Standard does not require chemical inventories; however, it is prudent to adopt

this practice. An annual inventory can reduce the number of unknowns and the tendency to stockpile

chemicals.

In Appendix A, you will find an inventory of the chemicals used in this laboratory. The inventory was

established in December 2011. It will be reviewed continually, but at least once per year, to insure that

new chemicals have been added and that those no longer in use have been removed. SeeAppendix B


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THE CHEMICAL HYGIENE PLAN (CHP)

INTRODUCTION

This document serves as the written CHP for The Community College of Philadelphia, Northeast

Regional Center Laboratories. The CHP is a regular, continuing effort, not a temporary or short term

activity.

SCOPE AND APPLICATION

The CHP applies to all personnel, both faculty and staff, in CCPs Biology/Chemistry Department and

laboratories engaged in the use of hazardous chemicals. The CHP must include the necessary work

practices, procedures and policies to ensure that employees are protected from all potentially hazardous

chemicals used or stored in their work area. Other laboratories of CCP may use this document as a basic

model to customize procedures and practices specific to their laboratories. Laboratory uses of chemicals

not covered by the CHP are subject to the full provisions of the OSHA Hazard Communication Standard.

RESPONSIBILITY

Supervisors are responsible for:

Implementing and maintaining the CHP in their respective work areas.

Providing means and motivations to allow all employees to comply with occupational safetyregulations.

Workers knowing and following the chemical hygiene rules.

Conducting any necessary Hazard Assessments and posting any necessary written Hazard Assessmentcertifications in each work area.

Keeping Personal Protective Equipment (PPE) and other protective equipment available and inworking order.

Providing appropriate information and training, including all PPE training.

Insuring that facilities and training are at all times appropriate and adequate.

The responsibilities of supervisors also include:Providing regular, formal chemical hygiene inspections of their facilities and equipment

Knowing the current legal requirements concerning regulated substances

Customizing the work area Chemical Hygiene Plan in any way necessary to provide for safetytraining, standard operating procedures, emergency procedures, etc and circumstances and/or

procedures and/or operations if the hazards of the laboratory work are not sufficiently addressed by

the CHP.


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In customizing the work area Chemical Hygiene Plan, it is only permissible for the supervisor to add and

clarify the requirements, not to omit or relax any which are given in the manual.

Laboratory workers are responsible for planning and conducting each operation in accordance with the

CHP procedures and for developing good personal chemical hygiene habits.

While students engaged in a specific courses laboratory are not covered under the provisions of the

OSHA Laboratory Standard, students should be made aware of chemical health and safety hazards in

classroom situations, and should be provided with information and equipment to protect themselves from

those hazards as needed. Teaching faculty should provide student training at the beginning of each course

in which hazardous chemicals are used. Specific safety instructions should be provided at the beginning

of each lab period.

EXPOSURE LIMITS

For laboratory uses of hazardous substances, departments must ensure that laboratory employees'

exposures to such substances do not exceed either the permissible exposure limits (PELs) specified in 29

CFR 1910, subpart Z, which are set by the Occupational Safety and Health Administration (OSHA), or

the Threshold Limit Values (TLVs) published by the American Conference of Governmental Industrial

Hygienists (ACGIH), whichever is lower.

PENNSYLVANIA WORKERS COMPENSATION NOTIFICATION REGARDING

WORK-RELATED INJURIES

This document required of all employees of the College at time of hire

(See next page)


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NOTICE TO EMPLOYEESIn accordance with Section 306 of the Pennsylvania Workers' Compensation Act, Community College of

Philadelphia designates a list of health care providers. When an employee is injured, the most important thing is to secureappropriate medical treatment. Once this has been done, the injury or claim should be reported according to the following steps.

Please read this notice carefully as it explains important rights.

IN CASE OF WORK-RELATED INJURIES

I. If you suffer a work-related injury, Community College of Philadelphia must pay for reasonable hospital treatment,

medicines, medical services and supplies, orthopedic appliances and prostheses, including training in their use, as

and when needed.

2. The injured employee has the responsibility of reporting the work-related injury immediately to the Security Office and

his/her department head or supervisor. This must be done as soon as an injury is realized or suspected.

3. It is a requirement to report all work injuries to the Human Resources Office in order to initiate claim.

4. In order to assure that your medical treatment will be paid by Community College of Philadelphia or Community

College of Philadelphia's current insurance company; you must treat with one of the designated physicians or medical

facilities listed on the attached list during the first 90 days after your first visit.

5 . You must continue to visit one of these healthcare providers I listed, if you need treatment for 90 days from the date

of your first visit. If you don't comply with this requirement, Community College of Philadelphia will be relieved

from liability for payment of services rendered during this period.

6. After the 90 day period described above, if you still need treatment, you may choose to go to another licensed

physician or medical facility for treatment or to continue with a College-designated healthcare provider. You must

notify the College's Human Resources Office within 5 days of your visit to a healthcare provider of your choice.

Failure to notify the College Human Resources Office will relieve Community College of Philadelphia from

liability for payment for services that are determined to have been unreasonable or unnecessary.

7. The physician or medical facility who treats you must file a Medical Report form issued by the (PA) Bureau of

Workers' Compensation with 10 days of the commencement of and at least once a month thereafter as long as

treatment continues. A copy of the report must be furnished to you and the Community College of Philadelphia Human

Resources Office. Community College of Philadelphia is not liable for payment for any treatment until a report has

been filed.

8. If a College-designated healthcare provider listed refers you to another licensed specialist, Community College of

Philadelphia or its insurer will pay the bill for these services.

9. If you are faced with a medical emergency, you may secure assistance from a hospital or physician or medical facility

of your choice. As soon as possible thereafter, please contact a College-designated health care provider for discussionof future treatment, if further treatment is needed.

I acknowledge receipt of this notice, which informs me of my rights and duties under thePennsylvania Workers' Compensation Act.

EMPLOYEE SIGNATURE __________________________ DATE _______________________

EMPLOYEE PRINT NAME _________________________ _____________________________

SOCIAL SECURITY NUMBER


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Original Copy: Human Resources Yellow Copy: Employee

PART 1

DEPARTMENT, FACULTY & STAFFDEPARTMENT INTRODUCTION

Presently, CCPs Northeast Regional Center Campus has five laboratories. These

laboratories are used by Biology/Chemistry/Physics students to conduct experiments

for the courses they are taking. The Regional Center offers a variety of courses such

as: Intro Chemistry 101 & 110 for Allied Health students; Bio-Chemistry 118; College

Chemistry 121, Biology 106, 107,109 & 110, Micro Biology 241 and the newly

developed Bio Tech program. The College offers these courses at the Main Campus,North East Regional Center [NERC], North West Regional Center [NWRC] and at

many smaller satellite campuses around the Philadelphia area. The college also offers

online courses, evening courses, weekend courses, 10 week sessions and accelerated 7

week sessions in the summer.

The courses are applicable for Associate Degree Programs in Arts, Science and

Applied Science, Certificate Programs, general education, etc. Matriculation

agreements with local universities and transfer of credit to other institutions is widely

accepted. During the year, the laboratory may hire and train a student laboratory

assistant (CCP student) to aid with support work. The goal of the department is to

operate safely and efficiently.

In performing these functions, we follow the safety guidelines outlined by the OSHA

Laboratory Standard. The implementation of this Chemical Hygiene Plan is the

primary concept of the OSHA Laboratory Standard. The Chemical Hygiene Plan

covers necessary work practices, procedures and policies, protection of employees

from hazardous chemicals, employee training and information, medical consultation,

hazard identification, respirator use and record keeping. See Appendix C for the

record keeping log.

Our goal is to have all parts of our operation in compliance with all of the areas

covered by the OSHA Laboratory Standard.


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SAFETY INFORMATION

Labels and Hazardous Identification

Labels on purchased chemical bottles have been developed to supply on-site safety

information (i.e. product identification, warning information, manufacturer, CAS#).

Manufacturers use a rating scale of 0-4 (0 = non-reactive; 4 = most reactive) and

pictograms to convey important health information. All purchased chemicals have

such a label.

Material Safety Data Sheets (MSDS)

MSDS is a reference source for chemicals which supplies current chemical and

physical data and health information on a particular chemical. MSDS sheets are

provided by the chemical supplier upon purchase of a chemical. The NERC campus

maintains a file of MSDS located on the shelf above the file cabinets in the laboratory

Prep Room (318). SeeAppendix A for further information.

Personal Protective Equipment (PPE)

The basic PPE used in this laboratory are ANSI (American National Standards

Institute) approved (ANSI Z87.1) safety goggles, lab coats, disposable gloves, dust

and vapor masks, partial face and full face shields and respirators. Safety goggles must

be worn in the laboratory. This is mandated by the Department and also a

Pennsylvania State law. See Appendix D Contact lenses are not approved safety eye-

wear and should not be worn in the laboratory. Other PPE is worn when the work

dictates.You should become familiar with the Safety Equipment and the spill clean-up

shelf, located in the Prep Room (318) and the lab in room 347. The shelves across

from the MSDS sheets contain reference books, as well as clean-up material. For

more information on ANSI,

SeeAppendix E

Eyewashes

Permanent Type

In each laboratory on the lecture bench in front of the sink is installed a

permanent eyewash. Note the operation instruction posted on the lecture bench in front

of this unit:

You have to pull up,squeeze and hold the handle, then release the handle to

shut off the water.


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Portable Type

There are portable units attached to the wall in Room 317 (Chemistry Lab)

and one unit in Room 316, 319 (Biology Labs). These units are located on the front

bench.

Floor Standing Pressurized Unit

There is a floor stand unit combined with a Safety Shower in Lab Rooms 339

and 347.

Safety Shower

A safety shower is located at the front door of each laboratory and Prep Room(318).

Fire Blanket

A fire blanket hangs at the front door of each laboratory. Although fire blankets may

have been used more frequently at a time when safety showers were not available,

they are still in use today.

Fire Extinguishers

A. The National Fire Prevention Agency (NFPA) has a scale from 0 (lowest) to 4

(highest) of the flammability of materials.4: Extremely flammable

3: Ignites at normal temperature

2: Ignites when moderately heated

1: Must preheat to burn

0: Will not burn

B. There are 4 types of fire extinguishers used in laboratories, each with a different

class rating. However, only Carbon Dioxide and Dry Chemical Extinauisher are

used at NERC.Dry Chemical: Class A, B, C

Carbon Dioxide: Class B, C

Halon 1211: Class B, C

Metal X: Class D

C. The 4 ClassesClass A: Wood, paper, trash

Class B: Liquids, grease

Class C: Electrical equipment

Class D: Metals

The fire extinguishers will state which class it can be used for. Generally, read the fire

extinguisher to know and understand how it can be used.


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D. There are, however, some benefits of using one type of extinguisher as opposed to another.

1. A Dry Chemical extinguisher can be used to build a dam around a solvent

spill or fire which will reduce the spread while attempting to clean up the spill or

extinguish the blaze. (Caution: make sure you stand far enough away from the

fire when using a Dry Chemical extinguisher, because the powder is very fine &

it can spread very easily. It is extremely messy to clean up).

2. A Carbon Dioxide extinguisher will leave no residue behind once the fire

has been extinguished. However, it will leave moisture which can cause further

damage to equipment through rusting or electrical shorts.

3. A Halon extinguisher will not leave any residue and will not cause further

damage to equipment through rusting or electrical shorts of electrical

equipment

4. Metal-X is the fourth type of fire extinguishing medium used in the

laboratory. Metal-X is used specifically for Class D metal fires such as sodium

metal, magnesium metal, etc. since this type of fire burns so hot it cannot be

extinguished by a traditional extinguisher. Metal-X is available both as a fire

extinguisher and as a powdered solid. The tank of the Metal-X fire

extinguisher is yellow, not your traditional red, and is located in the Storage

Room (W1-8B). The Metal-X powdered solid has been put into glass jars and

labeled accordingly. The jars are located on the spill clean-up shelf in the Prep

Room (W4-50).

Note: Fires once extinguished can reignite when using a Carbon Dioxide or Halon extinguisher.Another benefit of the Dry Chemical extinguisher is that it can prevent this from happening once

you cover the top of the burning substance with the powder. When using a Carbon Dioxide or

Halon extinguisher, you must stay ready for a few minutes in case of reignition. Never turn your

back on a fire.

Emergency Cut-Off Levers

It is extremely important that the staff learn the location of all the laboratory emergency cut-off

levers and safety equipment.

Emergency cut-offs for GAS:

Rooms 315, 316, 317, 318 and 319 gas cut off is on the side of the Instructors bench in the

laboratories. On the wall by the safety shower in the Prep Room (318).

Push in button to shut off Gas, pull out to turn on Gas.


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Rooms 339 & 347 All cut offs are at the front door push red button in to shut off gas and

electric to room. After the switch has been activated, a key must be obtained from Kathy Mulray

in the administration office, to restore power.

Emergency Lighting

Emergency lights are in the hallways outside of each laboratory. The emergency lights provide

light in the hallway in case of a power failure and provides ample illumination to allow students

and staff to shut down and exit the laboratory in an emergency situation.

Electrical Breaker Boxes

There is a master electrical breaker box located in Room 314, and 323 which are the Mechanical

Rooms at NERC. There is also a reset button on each electrical outlet. If a breaker should trip or

if there is some kind of electrical emergency then you can either reset the breaker or turn off the

breaker to the electrical line which is having the problem

Spill Clean-up

In the Policy and Procedures section, there is a Spill Clean-Up Procedure. Not all spills demand

an extensive procedure, so use your discretion. Also, you should become familiar with the area

(Prep Room 318, and Room 347) where we keep all the safety materials used in a spill clean-up.

If there is a spill, you should notify the lab supervisors immediately.

Fire Drill & Emergency Evacuation Procedure (Written by CCP)

A. At the sound of the evacuation alarm (a constant alert sound and flashing strobe lights), a

faculty or staff member should take the following steps:

Tell students to stop all work and shut the gas, air & water lines.

Instructor should shut only the emergency cut-off to the gas valve.

Instruct students to take their belongings and exit through the fire tower.

Now follow CCPsEmergency Evacuation Procedure (posted on the entrance doors):

Assign two individuals (faculty, staff or students) to accompany anyone who needs help due

to a disability to the nearest fire tower landing. Upon entering the tower:

One assistant will remain with the disabled individual.


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Other assistant note the landing location (this information and these instructions

have been placed at the landing) and proceed down the tower, exiting the

building.

If officials are on-site, report the location needing assistance to them.

If officials are not on-site, report to a Security Guard or Reception Desk the

location needing assistance.

Individuals with an impairment, but who are otherwise ambulatory, will wait at

the landing and descend after the foot traffic clears.

B. (Laboratories) Extinguish any open flames and pull fume hoods down.

C. All other individuals shall collect their belongings and exit the building through the nearest

fire tower.

D. Close and lock doors as you clear the area.

LABORATORY INFORMATION (North & East Buildings)

Laboratory Introduction

There are four basic laboratories, which are almost identical in their set-up, except for a few

differences. Room 316 is used for General Biology, Room 317 for Chemistry and Physics,Room 319 is used for Anatomy and Physiology, Room 339 is used for Anatomy and Physiology

and General Biology II, Room 347 is used for Microbiology and Biotechnology. The Prep

Rooms, (318, 347A, and 339A) are where we do most of our preparatory work. These rooms also

contains storage of materials that we use on a daily basis.

Prep Room

There is a wide variety of materials kept in the Prep Rooms (318, 347A, and 339A) because they

are used on a daily basis. Because glassware is used extensively by students and staff, it is storedin the cabinets over the sinks.. Other cabinets in the Prep Room contain various types of paper

materials for filtering, weighing, chromatography, etc. Reagents and indicators are also stored in

the cabinets and one of the Hoods. Various types of equipment are stored throughout the drawers

in the Prep Room. There are two hoods in Prep Room 318. One is to work in and one is for

storage only. No work is allowed in this hood. There is one hood in Prep Room 339A. In the

future we hope to have a ledger and schematic of all this equipment to make it easier to find.


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Ventilation Hoods

Ventilation hoods are located in each laboratory. There are two hoods in Rooms 316 and 317

and one hood in Rm 319. There is one electrical control box for each hood. These are singlesingle-speed hoods. What this means is that there is only one speed per hood. When the lab

hoods are on there is a flow of fresh replacement air that comes into the room through the hoods

duct work. This is called make-up air. When air is being exhausted from the room there needs

to be air coming from the outside to continue to allow the hood to exhaust. You will work under

these hoods when you are preparing solutions and reagents. There are two additional hoods in

the Prep Room (318), one to work in and the other for storage. No work is allowed in the Prep

Room storage hood. There is also one hood in Prep Room 339A. In addition, there are 3

biological safety cabinets located in Rooms 347, and 339. These hoods are designated for

biological materials and reagent preparation.If the hoods in any area are not working properly or

if there is some type of emergency that warrants a hood shut down then you can do so by

pushing the stop button on the control box in the Mechanical Room (314) (or by pressing the

emergency shut-off switch at the entrance of Rooms 339 and 347), and notify us immediately.

Glassware and Equipment

There are many different kinds of glassware in the laboratory, some of which is repairable and

some which is not. General glassware such as beakers, test tubes, flasks, graduated cylinders,

watch glasses, funnels, etc should be discarded at the first sign of any cracking, chipping or

visible breaks. Cracked or chipped glassware will break more easily during usage. Other piecesof glassware or equipment may be repaired or be salvageable such as suction flasks, Buchner

funnels, or burets. However, they must be removed immediately from general use to prevent

injury to students or lab staff. Some glassware items, for example the student burets, have one or

two additional moving parts attached. So, the buret glassware itself may be broken but the

attached parts may be in good condition and salvageable.

Storage Room

Our chemical and equipment storage areas are located on the third floor in Rooms 313 and 345.

The Storage Rooms house equipment as well as our stock of organic, inorganic chemicals. To be

permissible, the inorganics are stored on one shelf unit and the organics are on a separate shelf

unit. As long as these classes of chemicals are separated this type of arrangement is legal. The


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chemistry lab managers keep an inventory of all chemicals and equipment in the Storage Room.

See the complete chemical inventory inAppendix B . No one is allowed in the Storage Rooms

(313, 345) without first checking with the supervisors.

Waste Disposal

There are chemicals that pose no threat to the environment, and can therefore be disposed of

easily. However, there are items that simply cannot be discarded this easily and must be

disposed of properly. This requires the hiring of a licensed company to come on site and pick up

the waste. We do have a company that we hire to come on-site to do this type of disposal a

number of times in a year. They provide the proper packaging and documentation necessary to

pick-up and transport hazardous waste to an appropriate disposal facility. The chemistry

department lab has a yearly budget specifically for this purpose. Any waste collected in the

laboratories gets stored in a designated location under the Prep Room (318) storage hood. Thiswaste is stored to await pick-up. In the East building, microbiological waste is stored in the

biological safety cabinet in Room 347 for disinfection prior to disposal. In general, any

chemicals containing heavy metals must be collected and disposed of properly.

(See Appendix C).

Housekeeping

It is up to everyone to help keep the laboratories cleaned after your students have completed their

experiment. Please ensure that they cap and return all bottles to the hood, clean the glassware andreturn all equipment. We ask that you clean and shut-off the balances, check that all bottles are

capped, gas lines are off and water lines are shut on the benches, at the sinks and in the hoods. If

you are the last lab of the night, shut off the lights, lock the door and check it to ensure that it is

closed.

Laboratory Room Set-Up

The laboratories are set up with the experiment that you are performing in that week. All alike

groups (ex. Chem 110) will do the same experiment for that week. There are no back-ups, make-

ups, skips, or different experiments performed. However, there are some special considerations

due to closures or special college functions.When your class has finished, we clean up the

experiment and the room and then set-up for the next group. This is the ongoing process for the

week. Please finish on time, because there is no extra time between classes. Also, it may be


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necessary to either wait an extra few minutes before you enter or finish a few minutes early in

order for us to have enough time to reset the room for the next class SeeNew Instructors Policy

Semester Break

Over the semester break we clean and restock all student equipment lockers. We re-sort

peripheral equipment, check the back-up stock and check the function of additional equipment

that students use during their experiments. Also, we thoroughly clean the laboratories and check

the operation of the safety equipment. See the Semester Break Policy in the Laboratory Workers

Training (Part II) for a more detailed list of the maintenance that is performed. This policy gives

us the opportunity to test, check and correct any problems we may have before the new semester

begins. Also, see the Maintenance Check List inAppendix C.

Maintenance Check-List

The following is an overview of what maintenance we perform during the semester break. See

Semester Break Policy .

1. Restock student equipment lockers

2. Re-sort back-up & hood equipment

3. Replenish meter stick & glass tubing

4. Check & clean computers

5. Check for and replace burned out bulbs in ceiling

6. Clean & check balances

7. Clean benches

8. Clean out sinks & sink trough and replace netting

9. Clean or replace hood sink drain covers

10.Restock cleaning supplies under demo bench sink

11.Clean floors & empty trash

12.Check hood ventilation & electrical control boxes

13.Check for leaks under troughs

14.Check steam, air & gas valves

15.Check fire extinguishers

16.Check emergency cut-offs

17.Check aspirators

18.Check all eye washes

19.Check and operate safety showers

20.Check bench spigots

21.Check ice machine

22.Check water lines at sinks & in hoods


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23.Flush all water lines in the laboratory

24.Clean-up Prep Room

25.Check and calibrate all bench top equipment

LABORATORY WORKERS

INTRODUCTION TO TRAINING

The following section will cover individuals who want to work in the laboratory. They will be

trained in the procedures of the laboratory, such as safety, making solutions, handling chemicals

and the overall operation of the laboratory. Training will be performed in accordance with the

OSHA Laboratory Standard and the Chemical Hygiene Plan. It is necessary for you to become

familiar with the following information in order to work safely and effectively in the laboratory. Please

ensure that you follow all instructions herein and if you have any questions, please do not hesitate to ask.

LABORATORY INFORMATION

Under OSHA regulations , departments must provide employees with information and training to

ensure that they are apprised of the hazards of chemicals present in their work area and the steps

they should take to protect themselves from these hazards. Training may take the form of

individual instruction, group seminars, audio-visual presentations, handout material, or any

combination of the above. However, the training must include the specific hazards associated

with the chemicals in the work area when generic training is insufficient (e.g., extremely toxicmaterials, carcinogens, reproductive hazards, etc) to address specific hazards.

HIRING POLICY (written by Richard Sobel)

The hiring procedure shall be as follows:

1) CCP students interested in working should obtain from the laboratory an application and a schedule.

These should be completed by the student and returned to the laboratory along with a list of

Chemistry/Biology courses taken, your grades for these courses, your instructors for these courses,

and the name of the instructor, whom you are using as a reference.

2) The laboratory will hire new applicants based on the following criteria:

a) Your chemistry/biology background as indicated by your grades of completed chemistry


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/biology courses.

b) Any recommendations you may have from a CCP chemistry instructor.

c) Any laboratory proficiency as noted by the laboratory managers;

d) The present number of job openings available;

e) Your current class schedule;

f) The laboratory budget and the laboratory schedule;

3) All students working in the laboratory should have the following understanding:

a) They are employed on a semester to semester basis;

b) Hourly wages are established by the College Administration, not by the

laboratory.

c) Five unexcused absences from your work schedule can mandate your

termination. [ NOTE: Prior notice to the lab is acceptable; however, five or more

unannounced absences are not acceptable.]

d) Punctuality is a MUST. If your day begins at 10:00 AM, do not wander into the lab

at noon. You will be considered absent.

e) All assistants will obey department policies for safety;f) All laboratory personnel will wear "eye protection" while working in the laboratory.

4) Because of the nature of the work in the Laboratory, it should be noted that not all

laboratory assistants hired will be expected to be proficient in all the laboratory procedures and

techniques. The criteria for judging an applicant will vary depending on the position open and

the nature of the job opening.

The laboratory work shall be generally divided into two categories:

a) Assistants who function directly with laboratory chemical preparation;

b) Assistants who function as support personnel.

5) All regular payroll employees (gray time sheets) are permitted to work a maximum of 20 hours per

week during any regular semester, schedule and budget permitting. The maximum hours of work-study

(green time sheets) will be decided by the College, but cannot exceed 20 hours per week. When work-

study students have exhausted their funding, they ARE NOT automatically rehired as regular employees;

however, they may reapply for regular student status if applicable.

6) The laboratory employs several students during a regular semester. However, not all

Students are needed during the break between semesters and the summer sessions.

a) The laboratory assistants employed during this time will be selected according to their

ability, proficiency in chemistry/biology, and laboratory work habits.

b) The number of student assistants employed during the semester break and

summer sessions shall be determined by the work load of that particular time

period and shall be announced by the laboratory managers approximately two

weeks prior to that particular time period.

c) All student laboratory assistants working during this time will be employed for an unspecified

number of hours, provided, of course, that they are enrolled for summer classes and that the

laboratory is open for at least 4 days per week. (20 hrs max)


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d) According to college policy, all student assistants must currently be enrolled in at least one

Community College course. Student assistants who work during the summer sessions but are

not taking summer courses, must be pre-registered for the upcoming fall semester.

e) Any discrepancy between a student assistant and a faculty member will be taken up by

Department Head and the laboratory managers in a meeting at which time a decision will be made

determining the status of the student.

7) The laboratory managers reserve the right to determine the number of students necessary to

maintain a functional laboratory process. Any discrepancies with this policy will be taken into

consideration by the Department Chair and the laboratory managers.

8) All laboratory personnel must complete the safety training and laboratory training as outlined in the

CHP.

Medical ConditionIndividuals with a medical condition shall obtain a permission form from their doctor. They

shall fill out the form with their doctors approval listing any limiting factors of concern.

Working Papers

When you are hired to work in the laboratory (or any area of the College) you need to obtain working

papers. Please complete all the information pertaining to yourself and then give these papers to your

supervisor. When he/she has signed your working papers, they are to turn them into the Financial Aid

office for processing, which can take several weeks.

Time Sheets

All assistants need to complete a new time sheet by Thursday of each week (Wednesday during summer

sessions). The time sheet is to include your full name, J - number, pay ending date, and your signature.

The pay period ending date is always on Sunday. For example, if your first day of work during the week

is Tuesday, April 4, you will use the date April 9 (which is Sunday's date) as the pay period ending date.

Enter in the time you come to work and leave each day in the appropriate space and bubble in the

corresponding circle. Then mark the total hours worked for that week in the column labeled as such and

sign and print your name.

All time sheets must be filled out with a No.2 pencil and be error free; if not, the payroll department

will return the sheet for correction. This will result in your missing the current pay period. If that were the

case, you would have to wait until the next one. Pay periods are in 2-week cycles, therefore you would

have to wait 4 weeks to receive your paycheck.

Lab Attire White lab coats are required for working in the laboratory. Because you will be workingwith chemicals, you DO NOT want to wear nice and/or expensive clothing on days you are scheduled to


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work in the laboratory. However, we do ask that you be appropriately dressed with closed-toe shoes, full

socks, jeans (preferably without holes or tears). Note that tank tops, sleeveless shirts and offensive T-shirts are not appropriate.

Neither is long hair that is not tied back. Also, no loose-fitting or long-flowing items of clothing, baggy

pants, chains or scarves, as any of these aesthetic pieces of clothing are a potential safety hazard.

Initial Safety Practices

Safety is the number one priority in the laboratory. You will be expected to conduct yourself in a

professional manner and exhibit the utmost safety concern for yourself and everyone you work with.

Although you will be trained extensively in the laboratory and in safety, the following rules should be

adhered to immediately:

a)All lab assistants will wear protective goggles, lab coats, gloves and the necessary protective

Clothing when working in the laboratory.

b) When working with chemicals:

DO NOT Rub your face or eyes

DO NOT put pencils, pens or eyeglasses in your mouth

PROTECT open cuts and sores by keeping them covered with band-aids and gloves

DO NOT run authorized or unauthorized experiments without permission

KNOW THE LOCATION of emergency phone numbers next to each lab phone.

WASH HANDS regularly while working especially before leaving the lab

c) There is ABSOLUTELY

NO EATING

NO DRINKINGNO SMOKING

NO HORSEPLAY

NO RADIOs OR TVs

NO HANGING OUT

NO FRIENDS

NO CELL PHONE USE WHILE WORKING

Allowed in the laboratory at any time.

Staff and FacultyThe Department Head of the Chemistry Department has an office in room W4-46. The Biology

Department Head has an office in W2-5A. There are many full and part-time faculty members, co-ops

and up to a dozen lab assistants. Since there are so many people in both of the departments, it is

imperative to be as cooperative, courteous, and professional as you can.

Lockers


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You should obtain a locker at the College (if available) to keep your books and personal belongings

locked.The laboratory cannot be used to store books and personal items,nor do we take esponsibility for

anything lost or stolen. Be aware that these things can and do happen on a regular basis.

College Tour

Before you continue with the "Hands-On" portion of the training package, there are locations in the

college that you must learn.

DEPARTMENT LOCATION

Nerc Administative Offices Room 103

Bulletin Boards Outside Rms 318 & 319

Main Security (Lost and Found) Main Entrance (guards desk)

Copy Machine Room 301 K

Faculty Mailboxes Room 301 K

MAIN CAMPUS

Plant Department MG-2A (Subject to change)

Accounts Department M1-

Payroll M1-2

Purchasing Office M1-3

Copy Center MG-28

Mail Room Bonnell Ground TBA (Subject to change)

Admissions & Enrollment Services Bonnell Ground BG-5 (Subject to change)

Financial Aid Office Bonnell Ground BG-3 (Subject to change)

Cashier Bonnell Ground BG-1 (Subject to change)

Cafeteria Bonnell Ground BG-20 (Subject to change)

First Floor Storage Room W1-8B

Main Shipping, South Building Bonnell Bldg.

Freight Elevators West Bldg.

Security, West Building Entrance of the West Bldg.


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LABORATORY WORKERS SAFETY TRAINING (written by Ron Miraglia, 1988)

Labels and Hazardous Identification

There are labels on chemical bottles that have been developed to supply safety information on sight, such as;

- Product Identity

- Warning Information

- Manufacturer

NOTE: Manufacturers use a rating scale of 0-4 (0 =non-reactive; 4 =most reactive) and pictograms to

convey important information such as:

- Health

- Flammability

- Reactivity- Contact Hazard

- Statement of hazard / Flammability / Skin

- Explosion/ Radiation /Color code

- First Aid/ Bio-Hazard/Carcinogen

- Spill Instructions/ Protective equipment

READ LABELS - WHAT YOU LEARN YOU CAN TEACH TO OTHERS!!!

MSDS (Material Safety Data Sheets) and Reference Materials

The MSDS is a reference source for chemicals provided by the manufacturing companies which supplies the

currently available information on a particular chemical. In short, it is a character sheet that covers chemical,

physical, and health related data. There is a set of MSDS sheets above the file cabinets in the Prep Room (318)

See Appendix A for more information. Additional sets of MSDS books are available at the Security Desk, and

in Rooms 347A and 339.

Personal Protection Equipment (PPE)

Personal protection equipment encompasses a large variety of equipment. There is protective equipment

for everything in the hazard industry. What we will focus on is protective gear that we use in the

laboratory as follows: safety glasses and goggles, lab coats, gloves, dust and vapor masks, and

respirators.

Safety glassesSafety glasses or goggles must be worn in the laboratory. This is not onlymandatory in this department but is actually a Pennsylvania State law ( SeeAppendix D ).

Whenever you are working in the laboratory you must wear your safety glasses or goggles

including when washing glasswear. Contact lenses are not approved safety eyewear and are not

allowed to be worn in the laboratory.

Lab coats - Lab coats will be worn in the lab also. Lab coats add another layer of protection. If a

solution spills while you are working, it will generally hit the lab coat first which can be quickly

removed.


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GlovesGloves are also a necessary part of your protection whether you are working with

hazardous materials or chemicals in general. There are many different types of gloves in the

laboratory. The basic type of glove is disposable nitrile. This is the type that serves seventy

percent of our lab needs. Special jobs may require other more appropriate types of gloves. You

will learn the others if you encounter special jobs.

Facemasks and respiratorsThere are facemasks for dust and for odorous chemicals. Whenworking with these types of materials it is necessary to wear an appropriate facemask.

Respirators are available when working with materials that require more protection. Facemasks

are available on the shelf behind the door in the Prep Room (318). We will provide all the

necessary protective equipment while you are working in the chemistry laboratory. We expect

you to wear it.

These items are the staples of personal protection equipment in this laboratory. However, there are other

types of protective equipment that we use in the lab. You will learn them as you go through your training

and encounter their use when we are working with issues that warrant their use.

Spill Clean-Up

In the Policies and Procedures section, there is a Spill Clean-Up procedure. After you have completed

your training, we will extensively cover the Spill Clean-Up procedure. See the Policies and Procedures

section,

EyewashesTypes (Permanent, Portable, Floor Standing Pressurized, and Spigot Types)

1. Permanent Type:In each laboratory on the lecture bench in front of the sink is installed a permanent eyewash.

Note that there are instructions for use posted on the lecture bench in front of this unit. Permanent

eyewashes are checked for operation and flushed every four months.

To use the unit:

-Remove from holder

-Hold over sink

-Squeeze lever on the handle. Hold the lever down while in use.

To shut of the unit:

-Release lever

-Place back into holder

2. Portable Type:There are portable units in the laboratory. They are 1 L bottles of deionized water with a bactericide

additive to prevent bacteria growth. Portable eyewashes are emptied, cleaned, and refilled with DI water

and a bactericide additive every four months.

To use the unit:

-Remove one of the 1 L bottles from the wall. (Rm 317, on counter Rm 316, 319)

-Hold injured eye over the eye port and squeeze the bottle to flush deionized water into the eye.


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-Note that after each use the used bottle will be washed out, refilled and a bactericide additive is added

to help prevent bacteria growth.

Safety Shower

The Safety Showers are located inside the front door of each laboratory and by the hallway door in the

Prep Room (318). Each shower has an on/off pull handle. When the shower handle is pulled down thisengages the shower and copious amounts of water will begin to flow. In an emergency, all lab personnel

should rush to assist. To shut off the shower you must return the handle on the wall to its original upward

position.

Note that the showers in the laboratory do not have floor drains (except in 339 and 347). This requires

that the water on the floors must be mopped up after using any of the laboratory showers. Safety showers

are checked for operation and flushed every four months.

Fire Blanket

A fire blanket in a bag is hung inside the front door to each laboratory, and is available at the front of labs

339 and 347. It is possible to use the fire blanket to wrap a person who is on fire and roll them on the

floor to help extinguish the flames.

However, fire blankets were typically the only choice at a time when safety showers were not available.

So, a more logical first choice may be to use the safety shower.

Fire Extinguishers and Proper Usage

A. A. The National Fire Prevention Agency (NFPA) has a scale ranging from 0

(lowest) to 4 (highest) of flammability.

4: Extremely flammable

3: Ignites at normal temperature

2: Ignites when moderately heated

1: Must preheat to burn

0: Will not burn

B. There are two types of fire extinguishers used in the laboratory.

Dry Chemical - red tankClass A, B, C

Carbon Dioxide (C02)red tankClass B, C

C. There are four classes of fire for which these extinguishers are used.

1. Class A: wood, paper, trash

2. Class B: liquids, grease

3. Class C: electrical equipment

4. Class D: metals (e.g. sodium, magnesium, etc)

The fire extinguishers will state which class it can be used on. Generally, Dry Chemical is used to

extinguish Classes A. B and C fires. Carbon Dioxide and Halon for Classes B and C. Read the fire


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extinguisher to know and understand how they can be used.

D. There are however some benefits of using one type of extinguisher as opposed to

another.

1. A Dry Chemical extinguisher can be used to build a dam around a solvent spill or firewhich will reduce the spread while attempting to clean up the spill or extinguish the blaze.

2. A Carbon Dioxide extinguisher will leave no residue behind once the

fire has been extinguished. However, it will leave moisture which can cause

further damage to equipment through rusting or electrical shorts.

Note: Fires once extinguished can reignite when using a Carbon Dioxide

extinguisher. Another benefit of the Dry Chemical extinguisher is that it can

prevent this from happening once you cover the top of the burning substance withthe powder. When using a Carbon Dioxide extinguisher, you must stay

ready for a few minutes in case of reignition. Never turn your back on a fire.

HOW TO USE A FIRE EXTINGUISHER

Listed below is a logical and simple pattern of steps that can be employed when attempting to use a fire

extinguisher.

1. Take the fire extinguisher off the wall.

2. Break the plastic seal holding the pin in place.

3. Pull the pin out.

4. Stand back away from the fire approximately 6 to 8 feet.

5. Aim the extinguisher nozzle at the base of the flames.

6. Squeeze the handle to discharge the fire extinguisher.

7. While the extinguisher is discharging, move it from side to side, moderately, to cover the

entire base area of the flame.

NOTE:

1. CAUTION: Discharging an extinguisher can be forceful and loud.

2. It is sometimes not advisable to hold the cone of a Carbon Dioxide extinguisher

because the extreme cold temperature could cause severe burns on the hands.

3. Believe it or not, a five pound fire extinguisher has only 8 to 15 seconds of extinguishing power.

4. Each fire extinguisher has a range or area that it covers for the particular type of fire that you are

fighting. To make this information clearer, a chart that listing these ranges appears in the

appendix. ( See Appendix F )


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Large Fires

Procedures:

During the time of emergency, one cannot follow a long step-by-step procedure; therefore,

the simplest and most logical procedure to follow would be as follows:

- Warn Others

- Activate emergency cut off valve- Pull College Fire Alarm

-Contain fire, if possible, by closing doors that lead to other areas.

- Try to Extinguish Fire

- If you cannot control the fire evacuate immediately.

Note: It is important to know when to stay and fight the fire and when not to. This is called the Fight or Flight

principle. If you think that the fire is too big, call 911 and evacuate immediately.

Small Fires

Procedures:

There are many types of small fires in the laboratory. The examples below are some of the most common types

and the procedures to follow in the event they occur.

Example 1: Bunsen Burner Fire

- Shut off gas jet to Bunsen burner at the lab bench.

- Shut emergency cut off valve at the entrance door if you cannot reach gas jet.

Example 2: Beaker Fires on Bench tops

- Use ceramic board to smother fire. (do not try to pick up beaker and move it)

-Turn off gas jet and/or unplug hot plate.

Example 3: Paper or Notebook Fire

- Simply try to smother the fire

- Or pour water on the fireExample 4: Trashcan fire:

-Stand back away from the fire.

-Take a fire extinguisher and aim the cone directly at the fire at an angle.

-Do not aim the cone of the fire extinguisher over the fire. This will cause the flames

to shoot up into the air approximately 4-5 ft.

-Alternately, since the trash can is next to the sink you could easily take the water

hose from the lab sink, turn on the water, and extinguish the fire.

Note:

Small fires can quickly become larger and out of control quickly. As a fire burns it get hotter and hotter as the vapors

given off by the combustible materials begin to reach their flash point.

Fire Extinguisher Demo

During the new student safety lecture we will demonstrate how to use a fire extinguisher. A five- pound

Carbon Dioxide extinguisher is the only type we use. No other type of extinguisher is allowed to be used

in a demo These extinguishers are set up and labeled DEMO specifically for the safety lectures. We

never remove a fire extinguisher from the wall for demo purposes. It is actually illegal to remove a fire


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extinguisher from the wall for any purpose other than an emergency situation.

The Force of a Fire Extinguisher

Do not use a fire extinguisher on a small fire unless it is absolutely necessary. The force of the extinguisher can blow the

fire to the other side of the bench onto the students or spread the fire throughout the room. If the small fire cannot be

extinguished then carefully use a fire extinguisher by aiming the spray above the fire and slowly lowering the streamover the flame until oxygen supply is cut off. Another way to use the fire extinguisher in a beaker fire is positioning it on

the laboratory bench in front of the fire and aiming the hose just above the beakers. Strike the handle for a few short

burst of spray. Repeat this procedure ifnecessary. Small fires may be difficult to extinguish because of their nature.

However, in any situation, try to remain calm and use logic. SeeAppendix F for more detailed information.

Emergency Cut-Offs

It is extremely important that the laboratory staff learn the location of all the laboratory emergency cut-off handles and

safety equipment.

Emergency cut-offs for gas is located on the front bench in each lab and near the safety shower in the prep room.

To operate:

-Pull out red button to turn gas on.

-Push in red button to turn off.

The flow will stop in approximately thirty seconds. This is because the pressure in the line takes time to bleed out.

In rooms 339 and 347, the emergency cut-off is located on a panel at the entrance of each lab.

To operate:

Push in red button to turn off.

The electricity and gas flow will stop immediately.

To reset:

Obtain the key from the Administration office for the emergency cutoff switches.

Lab Ventilation Hoods & Make-Up Air

Ventilation hoods are located in each laboratory and the Prep Room of the North building. These are the single-speed

hoods. What this means is that there is only one speed per hood. When you turn on the hood it begins to exhaust.

When the lab hoods are on there is a flow of fresh replacement air that comes into the room through the hoods duct

work. This is called make-up air. When air is being exhausted from the room there needs to be air coming from the

outside to continue to allow the hood to exhaust. You will work under these hoods when you are preparing solutions

and reagents. There are two hoods in the Prep Room (318.) one is used for chemical storage. No work is allowed in the

storage hood. There are three hoods in the 339 and 347, and one single speed hood in prep room 339A. If the hoods in

any area are not working properly or if there is some type of emergency that warrants a hood shut down then you can do

so by pushing the stop button on the control box in the Mechanical Rooms (314, 323) or by pushing the emergency

cutoff valve in rooms 339 and 347.

Emergency Lighting

Emergency lights are in the hallways outside if each laboratory. The emergency lights provide

light in the hallway in case of a power failure and provides ample illumination to allow students

and staff to shut down and exit the laboratory in an emergency situation.


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Electrical Breaker Boxes

There is a master electrical breaker box located in Rooms 314 and 323 which are the Mechanical

Rooms. There is also a reset button on each electrical outlet. If a breaker should trip or if there is

some kind of electrical emergency then you can either reset the breaker or turn off the breaker to

the electrical line which is having the problem

Chemical and Equipment Storage

Our chemical and equipment storage areas are located in Rooms 313 and 345. The Storage Room s house

equipment as well as our stock of organic& inorganic chemicals. The organic and inorganic chemicals

are arranged in a permissable way in order to be stored in the same room. To be permissible, the

inorganics and organics are stored on two separated shelf units. As long as these classes of chemicals are

separated this type of arrangement is legal. The lab managers keep an inventory of all chemicals and

equipment in the Storage Rooms.

There are some basic rules that must be followed when lab employees go to the Storage Room:-No one is allowed to go without authorization from the lab managers.

-No one is allowed to go to the chemical Storage Room alone.

-Protective clothing must be worn.

-Take appropriate safety equipment (Retrieval cart with fire extinguisher, eyewash, and paper

towels)

-Open door and wait some minutes for air flow to flush excess vapor from the room.

-Note where fire extinquishers in the room are located.

-Note where spill clean-up materials are located.

-Note where eye wash units, face masks, and gloves are located.

-Report anything unusual, breakages or spills immediately.

-Retrieve or return specific items as needed then count the total of these same items that

remain on the shelf.

-Before leaving be sure to check that everthing looks OK.

-Shut the light and make sure that the door is locked.

Prep Room and Storage

The Prep Rooms (318, 339A, and 347A) are not storage areas, however, There is a wide variety of

materials kept in the Prep Rooms because they are used on a daily basis. Because glassware is

used extensively by students and staff, it is stored in the cabinets over the sinks.. Other cabinets

in the Prep Room contain various types of paper materials for filtering, weighing,

chromatography, etc. Reagents and indicators are also stored in the cabinets and one of theHoods Various types of equipment are stored throughout the drawers in the Prep Room. There

are two hoods in the Prep Room 318, three in 339/339A, and one in 347. Acids,Bases and

Solvents are stored under the hoods in 318 and 339A. In the future we hope to have a ledger and

schematic of all this equipment to make it easier to find.

.


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Glassware and Equipment

Glassware: There are many different kinds of glassware in the laboratory, some of which is repairble and

some which is not. General glassware such as beakers, test tubes, flasks, graduated cylinders, watch

glasses, funnels, etc should be discarded at the first sign of any cracking, chipping or visible breaks.

There is a specific broken glassware receptacle for this purpose in each laboratory and the Prep Rooms

(318, 339A, and 347A). Cracked or chipped glassware will break more easily, especially during use andcleaning. Other pieces of glassware or equipment may be repaired or be salvageable such as suction

flasks, Buchner funnels, or burets. However, they must be removed immediately from general use to

prevent injury to students or lab staff. Some glassware items, for example the student burets, have one or

two additional moving parts attached. So, the buret glassware itself may be broken but the attached parts

may be in good condition and salvageable.

Equipment: There are many pieces of equipment in the laboratory, too many to mention here. As you

encounter the various pieces of equipment you will learn their operation and functions. Some of the basic

day to day types of equipment will be explained in the trianing portion of this package. We ask that you

respect the laboratory and its equipment and that you not touch or attempt to operate any equipment until

you have been trained and have become familiar with it. Do not hesitate to ask questions about new

equipment. We will gladly take the time to explain things to you. And remember, there is no such thing

as a stupid question nor does the word assume exist in the laboratory

Housekeeping

Remember, it is up to everyone to work in the laboratory in a clean and safe manner. Your work area

should be kept clean and free from obstructions at all times. When you are finished your prep work or

whatever task that you are responsible for please return your chemicals and equipment back to where it

belongs. Finally, clean your glassware and your work area.

LABORATORY WORKERS HANDS-ON TRAINING(written by Ron Miraglia, 1988)

Orientation to the Laboratory and Training

In this section you will acquire some hands-on experience that will get you acclimated to the laboratory.

In the first part you will locate the equipment and reagents in the lab. The second part is to perform actual

work that you will be repeatedly involved with. Before you begin, I would like to give you a brief

description of the laboratory.

1) There are five basic laboratories, which are almost identical in their set-up, except for a fewdifferences. Room 316 is used for General Biology, Room 317 for Chemistry and Physics,

Room 319 is used for Anatomy and Physiology, Room 339 is used for Anatomy and

Physiology and General Biology II, and Room 347 is used for Microbiology and

Biotechnology. Finally, the Prep Rooms (318, 339, 347) are where we do most of our

preparatory work. These rooms also contains storage of materials that we use on a daily

basis


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2) There is a wide variety of materials kept in the Prep Rooms (318, 339A, and 347A) because

they are used on a daily basis. Because glassware is used extensively by students and staff, it is

stored in the cabinets over the sinks.. Other cabinets in the Prep Room contain various types of

paper materials for filtering, weighing, chromatography, etc. Reagents and indicators are also

stored in the cabinets and one of the Hoods Various types of equipment are stored throughout the

drawers in the Prep Room. There are two hoods in the Prep Room 318, three in 339/339A, and

one in 347. In the future we hope to have a ledger and schematic of all this equipment to make it

easier to find.

3) Our chemical and equipment storage area is located on the third floor in Rooms 313 and 345.

The Storage Rooms house equipment as well as our stock of organic, inorganic chemicals. To be

permissible, the inorganics are stored on one shelf unit and the organics are on a separate shelf

unit. As long as these classes of chemicals are separated this type of arrangement is legal. The

chemistry lab managers keep an inventory of all chemicals and equipment in the Storage Room.

See the complete chemical inventory inAppendix B . No one is allowed in the Storage Rooms

(313, 345) without first checking with the supervisors.

4) There are two hoods in the Prep Room 318, one used for storage and one to work in There

are three hoods in 339/339A, and one in 347.

5) Above the desk in the 318 prep room is a shelf with Reference books(Merck etc.) and

catalogs

Everything in the laboratory is under lock and key. It is important to keep it that way.

Learn the operation of the laboratory and keep it organized and secure.

Schematic of the Laboratory

At this point you will fill locate and identify most of the equipment in the laboratory and storage areas.

But before you do, we have to point out certain safety and necessary equipment that must become very

familiar to you.

a) Fire Extinguisher

b) Emergency Eye Wash (stationary)

c) Emergency Eye Wash (portable, wall and floor)

d) Emergency Showere) Emergency Cut-Off valves for the following:

1.Gas/Electricity

f) Electrical Breaker Boxes

g) Ventilation hoods and On/Off Electrical Boxes

h) Material Safety Data Sheets (MSDS)

i) Emergency Reference Books


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j) Spill Control and Clean Up Materials (Rm 318, 347)

k) Safety Material s (Rms 318, 347)

Equipment List

1- Flask, Erlenmeyer, 500 ml

2- Flasks, Erlenmeyer, 250 ml



1- Wire Gauze, ceramic center

1- Flask, Florence, 500 ml

1- Beaker, 1000 ml, 600 ml, 400 ml, 250 ml, 150 ml, 100 ml, 50 ml

1- Wash Bottle, plastic

6- Test Tubes, large

6- Test Tubes, small

1- File

3- Droppers

1- Funnel1- Evaporating Dish

1- Crucible with Cover

1- Thermometer, red alcohol

1- Clamp, test tube holder

1- Cylinder, Graduated, 100

1- Cylinder, Graduated, 50 ml



1- Tong, crucible

1-Watch Glass

1- Triangle, clay

1- Spatula

1- Striker


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2- Stirring Rod


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Hands-On Training

Next you will begin using the balances to weigh objects and chemicals.

Steps:

-Obtain weighing paper and the object to weigh.

-Ensure that the electronic balance is clean

-Turn on balance and wait to zero

-Place a weighing paper on the balance pan

-When the weight stabilizes tare the weight and weigh your object

-When finished turn off the balance and replace the cover

-If weight is too heavy then get a triple-beam balance from the Merck cabinet

-Adjust it to zero and then weigh your object in the same way as on the electronic balance.

Using the Balance:

a) Weighing Objects

Weigh four metal cylinders and report the mass. (Indicate which ones are too heavy for the electronic balance).

Use a triple beam balance if necessary.

Copper ________________________________ _

Tin _____________________________________ _

Zinc __________________________________ _

Iron _____________________________________

Weigh the following objects and report their mass.

1000 ml beaker __________________________________

600 ml beaker ________________________________

400 ml beaker ________________________________

250 ml beaker ________________________________

150 ml beaker _____________________________________________

100 ml beaker _____________________________ _

50 ml beaker _________________________________

Note: If you exceed the capacity of the electronic balance you will then be required to use the

Triple-Beam balance. This type of balance is simpler in the sense that you manually slide all the

moveable weights completely to the left to the zero position which causes the marker on the


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right to also read zero. Once you place the item to be weighed on the balance you can re-adjust

the weights by sliding them to the right until you reach the point where the marker on the right is

re-aligned back to the zero position, then read your weight.

b) Weighing Solid Chemicals:

In the following step, you will weigh out a sample of Sodium Chloride (NaCl), place it in a solid bottle and label

it. You will do this four times using different quantities and different bottles.

1. Obtain Sodium Chloride (NaCl) from the stock cabinet.

2. Weigh the required amount using a weighing boat or weighing paper and label the boat or paper.

3. Label the bottle before placing the chemical in it. Never fill a bottle or a jar before it is labeled. This

is how a label should appear:

SODIUM CHLORIDE

NaCl

F. W. 56.43

4. Place the sample in the bottle and cap the bottle. Prepare the following samples:

Quantities Bottle Size

1. 0.02 grams 60 grams




Chemical Preparations:In this section, you will get the hands-on experience that is necessary for you to get acclimated to the laboratory.

Equipment Demo Set-ups: (Part A)At this point you will be introduced to the common lab equipment and instructed on the use of such. Also

you will learn to set up general lab apparatus that students continually work with, such as:

a) Heat Lamps

b) Melting Point Apparatusc) Centrifuge

Preparing Solutions:Now that you have some familiarity with the laboratory, the equipment and the chemicals, you can

begin preparing chemicals and solutions. The following is a step-by-step procedure

Steps:

1. Obtain a volumetric flask, funnel, stirrer, stirring bar, and magnetic stirrer.2. Wash flask, stirring bar and funnel and rinse with tap water three times, and then rinse three times with

de-ionized water.

3. Place on magnetic stirrer with stirring bar in the flask and fill flask just under half full with de-ionizedwater. Start stirrer and place a temporary label on the flask.


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4. Obtain solute, weighing boat and spatula. Make sure that the spatula is clean.5. Using the balance that you have made sure is clean, turn it on and wait for it to zero. Place weighing

boat on the pan. Tare the weight of the boat and begin weighing the amount of material that you need.

Take the molecular weight off of the bottle, not the periodic chart.

6. Once you have weighed out the amount of material that you need, take the flask and begin putting thesolute into the flask using a funnel. Start the magnetic stirrer. You may not be able to add all of yoursolute because too much will stop the stirring bar from turning. You will need to be the judge. You

may need to add more water to the flask to help the solute dissolve.

7. While your solution is mixing you should clean and turn off the balance. Put the cap back on the jar ofthe chemical that you used and return the jar to the stock cabinet.

8. Select the proper size bottle for the solution that you are making, wash and rinse it and then label itproperly. Print in capital letters (except formulas) using black or blue ink only.

9. When all the solute has mixed into solution, remove the stirring bar by using a horseshoe magnetliftflash off of the stirrerplace magnet against the flask to attract the stirring bar. Slide the magnet and

bar up past the meniscus line and top off the water level. (Note that the bottom of the water level should

line up with the meniscus on the neck of the flask.) Then replace the stirring bar and continue to mix

until the solution is homogeneous then stop mixing and transfer to the properly labeled bottle and cap it.

Clean up the mess and put away your equipment.

Preparing Different Types of Solutions:

a) Common TypesPrepare a 2-liter solution of1.0 M Sodium Chloride (NaCl). Split the solution into a 1000 ml

bottle and two 500 ml bottles. Label the bottles.

Prepare a 500 ml solution of 0.1 M Sodium Chloride (NaCl). Split the solution into a 250 ml

bottle, a 125 ml bottle and two 60 ml bottles.


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b) Unknown TypePrepare a 500 ml solution of Copper Sulfate

(CuSO45H2O) and place it in the appropriate bottle.

c) Endothermic TypePrepare a 250 ml solution of 1.0 M Sodium Sulfate (Na 2SO4).

d) Large Particle Solid TypePrepare a 500 ml solution of 0.1 M Iron (II) Chloride (FeCl 2) and leave it in the volumetric flask.

e) Low Solubility Type

Prepare a 250 ml solution of 0.01 M I2 (Iodine) and observe the time until the solute dissolves.

f) Multiple Solute Type

Prepare a 1 liter solution of 0.1 Sodium Chloride (NaCl), 0.1 M Potassium Chloride (KCl) and

0.1 M Calcium Chloride (CaCl2). Leave the solution in the volumetric flask.

g) Slow Dissolve Type (with help)

Prepare a 500 ml solution of 0.01 M I 2 (Iodine) Solution. But, before you add the I 2, add 5 gramsof KI (Potassium Iodine) (Same as e above, but with the addition of KI to help the I2 dissolve)


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Diluting Acids and Bases:

NOTE BEFORE YOU BEGIN - Three things to remember when working with acids and bases:1. ALWAYS add acids and bases to water.2. ALWAYS work in the hood with sufficient protective clothing and a proper set-up.3. Use extra caution when making these dilutions. Some acids and bases have very strong odors.

They work very quickly to destroy what they come in contact with. Wash quickly with plenty of water

if you come in contact with any acid or base.

Use a cart to obtain the following equipment:

-small plastic tray

-glassware needed for measuring

-plastic eye droppers

-volumetric Flask, 1L

-acid or base that you will be diluting

nerc chem hyg plan micro

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