ncRNAncRNA

Jeremy LogueJeremy Logue

The Expanding Repertoire of RNA

Adapted from Soares and Valcarcel (2006) EMBO

Adapted from Huttenhofer, A et al. (2005) Trends in Genetics

The Expanding World of Non-coding RNAs

HAR1-associated transcripts in genomic context

Of the 49 HARS identified (Human Accelerated Regions),96% are in non-coding regions.

Predicted RNA secondary structure for HAR1F

Expression of HAR1F and HAR1R in the developing neocortex

Cajal-Retzius cells establish early neuronal circuitry in the developing brain

Expression of HAR1F in other parts of the developing brain

What does this all mean?

Fill in the blank…

X - InactivationX - Inactivation

Dosage Compensation

Adapted from Straub and Becker (2007) Nature Reviews Genetics

Imprinted Versus Random X-inactivation

Adapted from Avnir and Heard (2001) Nature Reviews Genetics

The X-inactivation Center

Adapted from Avnir and Heard (2001) Nature Reviews Genetics

Differentiation

Xist Transcription in Embryonic Stem Cells

Adapted from Avnir and Heard (2001) Nature Reviews Genetics

48 hrs.

Adapted from Avnir and Heard (2001) Nature Reviews Genetics

Potential Roles for Xist Antisense Transcription

What does a silenced X look like?

Adapted from Chow, JC et al. (2005) Annu. Rev. Genomic Hum. Genet.

Co-localization and 3D - Microscopy

How confident are we that these co-localize?

Figure 1G from Chaumeil, J. et al. (2007) Gen Dev

Problems Associated with Co-localization

Resolution = λ/(2NA)

z-axis problem

Calculating a cross-correlation can also aid in determining co-localization

3D - Microscopy