1

Natural Anti-Dandruff

Product Name: BCX-D

B103, WOOLIMLIONS VALLEY 2nd B-LINE, SANGDAEWON-DONG, JUNGWON-GU, SEONGNAM, GYENGGI-DO, SOUTH KOREA, 462-120 Tel:+82 2 573 8575 Email: szhen@ibst.kr

Natural anti dandruff shampoo is possible.

Biotransformation tech. is applied

The effect of BCX-D is equal to 50% Zinc pyrithione(P. ovale growth inhibition)

BCX-D is water soluable but Zinc pyrithione is not.

BCX-D Patented

BCX-D

INCI NameChamaecyparis Obtusa Leaf Extract,Cordyceps sinensis Extract,Lactobacillus Ferment

- Appearance : Light Brown Liquid- pH(2% Solution) : 6.7 ± 0.5- Soluble : in Water- Application : BCX-D is Natural Anti-Dandruff ingredient made

using Chamaecyparis obtusa and Cordyceps sinesis- Indicated substance: Terpene Bio

Conversion

BCX-D

Anti P. ovale

Anti-inflammation

Anti-Dandruff

4

Reduce high molecule of active ingredient of extract

of Chamaecyparis obtusa leaf was converted to low

molecule by fermentation, which increase the

inhibition of P.ovale and the transdermal absorption.

simultaneously reduce the toxicity and improve the

physical property.

1. Bio conversion (BCX-D) can

Before After

BCX-D Zinc pyrithione(50% sol.)

BCX-D 10% Zn Pyrithione10%

2. Comparison with Zinc Pyrithione

: The natural extract of Chamaecyparis obtusa leaf and Cordyceps sinensis was converted with Lactobaillucs plantarum and its efficacy was better than hinoki oil and similar with zinc pyrithione.

1) BCX-D and zinc pyrithione shows similar efficacy for inhibiting P. ovale.

***Notice typical zinc pyrithione is sold as 50% diluted form

2) BCX-D is water soluble

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Inhibition rate of 5% BCX-D and 2.5% zinc pyrithione was almost same.

3. Growth Inhibition of P.ovale: 3 days incubation

4. Anti-dandruff and anti-athelete’s foot activity – KTR test

Anti-fungus activity(Halo test : M. furfur, 48hr later)

BCX-DZinc pyrithione

10 %

0

0.4

0.8

1.2

1.6

BCX-D 5% zinc pyrithione

2.5%

before

bioconverted

control

O.D

*** Notice typical zinc pyrithione is sold as 50% diluted form

Anti-fungus activity (Halo test, T. rubrum, 7 days later)

Anti-dandruff activity Anti-athelete’s foot activity

BCX-DTerbinafine 5 %

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Stability

Bio-assay of BCX-D stored at 45 o C for a month was nearly same as that of stored at room temperature for a month, which confirmed the heat stability of BCX-D

1. 45 o C for a month 2. Room temperature

for a month

4o Cfor month

25o Cfor a month

45o Cfor a month

Precipitation No precipitation No precipitation No precipitation

Bio-activity No variance No variance No variance

When BCX-D was stored at 4o C, 25o C and 45o C, the precipitation and bio-activity variance were not observed for a month.

Formulas

Results of evaluation(No. of subjects)

Assessment Grade30mins

1hrs 24hrs

- - ± + +++++

- ± + +++++

Vehicle 10 10 - - - - 10 - - - - Non irri. 1

BCX-D 50% 10 10 - - - - 10 - - - - Non irri. 1

• Test Period: October 17,2013 - October 20,2013

• Purpose : To evaluate the safety of “BCX-D” in cosmetic

# Evaluatuon : ± Doubtful reaction+ Weak reaction (erythma, papoule)++ Strong reaction (erythma, papoule,

vesicle)- Negative reaction

• Any irritation was not obseved in BCX-D

• According to the above test result, BCX-D was considered to be a

extremely safe BCX-D

* Vehicle : Basic Lotion

Human Patch Test

Patent

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Patent No. 10-2015-0024476

Patentee : BST Inc.

Title of invention : The composition of Chamaecyparis obtusa leaf extract basis for dandruff and athlet’s foot.