nanotek & expo 2014 biodegradable nanobrushes for drug delivery eggehard holler, hui ding,...
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Nanotek & Expo 2014
Biodegradable nanobrushes for drug delivery
Eggehard Holler, Hui Ding, Ramachandran Murali, Julia Y. Ljubimova
Cedars-Sinai Medical Center, Los Angeles, USA
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Drug
Targeting Agent
Imaging Agent
Linker
Dendrimer Carbon Nanotube
Micelle
Gold Nanoparticle
Third generation ofNano-based Carriers
For Disease Detectionand Therapy
NanoparticleLiposome
Polymer- Conjugate
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Synthesis and degradation
FromGlucose
OHHOOCn = 500 to 3000
Physarum:
HOOC OHn = 40 to 80
Aureobasidium:
Poly(β-L-malic acid)
Depolymerisation:Spontaneous& Enzymatic
MalatePurification
Plastic bags Medical support
Drug deliveryMalignancy
Infection
Contrast agentsImaging
Nano scale
αβ
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Antisense Oligonucleotide
Monoclonal Antibody (mAb)
Disulfide, cleaved byGlutathion in the
Cytoplasm
Polymalic acid-trileucine copolymer
Prodrug
Targeting
O CH1
C H22
CO
OH
C3
O
O4
CH5
C H2
CO
NH
C OH
O n
Leu-Leu-LeuCOOH
Polymalic acid-trileucine copolymer
Polycefin – Functional Core
HO-CH-CH2-C-OH
O=C
OH=
O
L-Malic acid
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Anti-EFGR mAb(Cetuximab)
Anti-mouse TfR mAb
PEG
Antisense RNAEGFR
Cancer cell
Late Endosome
Early EndosomeGluta-thione
Nucleus
Akt
Antisense
EGFR mRNA
EGFR signalling
Endocytosis
EGFR
Active via receptor Extravasation
Mouse TfR
I.V. Injection
Blood vessel
PO4
EPR -effect passive
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Preparation of nanodrugs for brain tumor treatment
GBM: Chain-α4 Chain-β1 ( - ) anti-MsTfRmAb anti-HuTfRmAb HER2-positive breast: HER2 ( - ) ( - ) (anti-MsTfRmAb) Herceptin
Triple-negative breast: EGFR ( - ) ( - ) (anti-Ms-TfRmAb) Cetuximab
O O
O OO O OOH OH OH
O O O O O O O O
O O O O O O OO O O O O O O ONH NH NH NH NH NH NH OH
O
CH3
O
SSS S S
OO NH
SS S S
OHN OHN OHN PEG3400
O O
O O
PEG3400
O O
O O
S S
CH3CH3
O O O O
O O OO O O ONH NH NH OH
O
CH3
O
SHCH3
CH3
O
O O
Step-2
Step-1
mAb-1 mAb-2
AON-1
AON-2 AON-3
Polymalic acid
mPEG5000
mPEG5000
Alx-680optional
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Primary Triple Negative Breast Cancer
Primary HER2-Positive Breast Cancer
Primary breast cancer Brain metastases
47% survival rate improvementMetastatic HER2-Positive Breast Cancer
Surv
ival
%
0 25 50 75 1000
50
100
79% survival rate improvement
Surv
ival
%
0 10 20 30 40 50 60 70 800
50
100
Days
Metastatic Triple Negative Breast Cancer
Examples for drug delivery by Polycefin variants
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Replacement of antibody by affinity peptide: Pro and Contra
Pro Contra
Multiple peptides per conjugate
Less affinity to bind to target
Robust structure Absence of Fc and biological activity
Small size for slender shape of polymeric nanoconjugate
Less passive tissue targeting (EPR)
Increased diffusibilityDeep tissue penetration
Low Stability/reduced longevity in plasma
Reduced immunogenicityHumanization not required
Possibility of unscheduled side reactions
Possibility of multivalency Tendency for aggregation
Chemical fabrication
Easy packaging and delivery.Decreased overall MW of nanodrug and less injectable drug volumen
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AngiopepMichel Demeule, et al. (2008) J Parm Exp Therapeut
Target on BBB endothelial cells: LRP-1Guangqing X and Liang-Shang G (2013) Int J Cell Biol
Targeting Brain tumor
1. Example
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For Uptake and Imaging: P/PEG2000-Angiopep(2%)/AlexaFluor 680 (0.5%)
Glioblastomacell
Late Endosome
Early Endosome
Nucleus
Extravasation through BBB into glioblastoma
I.V. Injection
Blood vessel
Angiopep
PEG2000
P =
poly
mal
ic a
cid
AlexaFluore 860
Endocytosis
LRP-1LRP-1
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PMLSA-Angiopep-2: P/PEG2000-Angiopep(2%)/AlexaFluor 680
Fluorescence Imaging of Glioblastoma-Nude Mouse Model
Tumor
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Specific problem with affinity peptides:
Self-association and aggregation because of: Electrostatic complementation
Lipophilic amino acids
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OH OHNH
O CH3
CH3
NH
S
NH NH
S
S
AON
R
LLLLLL
OH
NH
O CH3
CH3NH
O CH3
CH3OH
NH
O CH3
CH3N
C
O
OOC
O
OO C
O
OOC
O
OOC
O
OOC
O
OOC
O
OOC
O
OO C
O
OOC
O
OO
PEG3400
NO O
S
NO O
AlexaFluoR 860
H
O CH3
CH3NH
O CH3
CH3OH
OHOH
OHOH2
Nanoconjugate250-500 kDa
(40%) (40%)
(2%) (0.5%)(2%)
Target: HER2Ramachandran Murali et al. (2001) J Med Chem
StarPEG(PEG200AHNP)2
10 kDaR =
2. Example
AHNP
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* p-value calculated by Two-Tailed T-Test = 0.034
In vivo Imaging ofSubcutaneous BT-474 Human Breast Tumor on Nude Mice
P/StarPEG(-PEG200AHNP)2
(2%)/LLL(40%)P/StarPEG(2%)
/LLL(40%)P/PEG200-AHNP(2%)
P/PEG200-AHNP(2%)/LLL (40%)
*p<0.05
Effi
cien
cy (
cm2)
-1E-06
2E-22
1E-06
2E-06
3E-06
4E-06
5E-06
P/StarPEG-(PEG200(AHNP)2
(2%)/LLL(40%)P/StarPEG(2%)
/LLL(40%)P/PEG200
AHNP(2%)P/PEG200
AHNP(2%)/LLL (40%)
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Code kon (s-1M-1) koff (10-3 sec-1) Kd(10-6 M)
AHNP 800 0.42 0.52
StarPEG -AHNP2
118 0.54 4.6
StarPEG -AHNP4
1.5 0.62 41
StarPEG-AHNP6
n.d. n.d. n.d.
StarPEG-AHNP8
n.d. n.d. n.d.
Surface Plasmon Resonance kinetics
Surface Plasmon Resonance kinetic parameters Dynamic light scatter (DLS)
Ln Ln-1 + L
L
Rate limiting dissociationof self-aggregate
(1)
L + HER2
HER2-L
(2)
(2)
(1)
StarPEG(AHNP)n
Hydrodynamic diameter (nm)
n = 2
n = 4
n = 8
n = 0
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Conclusion:
(1) Polymalic acid is qualified for peptide targeting
(2) Need of appropriate linkers
Syntheses: Hui DingImaging: Pallavi Gangalum
Martz Discovery Fund