nanorobots used in cancer treatment
TRANSCRIPT
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Bacteria based micro robot used in cancer treatment
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Korean research team has successfully developed First Cancer-treating Nanorobot
http://www.youtube.com/watch?v=VYwLy2Jb8Ik
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Introduction
• Cancer is the disease caused by an uncontrolled division of abnormal cells in a part of the body
• Chemotherapy , is the conventional treatment but..
Damage growing-healthy normal cells blood and hair cells
Not specific and No continuous drug delivery for tissues
Negative side effects ( anemia , nausea )
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Nano robots in medicine
• This research offer an alternative way instead of chemotherapy
• Bacteria based micro robot ( Bacteriobot )
• Bacteriobot = genetically engineered Bacteria + micro structure bead ( drug )
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Why Nano robots ?
• Specific .. target cancer cells but not normal cells
• Fast .. one day make sense with cancer treatment
• Depends on biodegradable and biocompatible materials
• optimal drug quantities delivered to the target cells
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How bacteriobot works?
• To be effective , bacteriobot moves toward tumors acting as combination of
• Micro sensor
• Micro actuator
• Therapeutic agent
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Designing the experiment
• Tumor model : colorectal cancer , breast cancer 4T1
• Animal model : CT_26 mouse
• Bacteria model : ppGpp S. typhimurium strain SHJ2037
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Making the bacteriobot
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How to combine Salmonella to capsule ?
• To deliver drug to tumor cells
• Salmonella work as mechanical engine
• Micro capsule work as drug container
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Engineering the bacteriobot
• It is a fusion between RT* and BT* presents a novel anti tumor strategy
• Stage 1 : Interaction between biotin – streptavidin
• Biotin found in all living organisms
• Attenuated salmonella genetically modified to express biotin on the outer membrane of the cell
* RT : Robot technology
* BT : Biotechnology
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• High affinity interaction between biotin – streptavidin
• Biotin can conjugate to many proteins easily let that protein to interact to with streptavidin conjugated molecules
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• Stage 2 : Development of flow-free chemotactic microfluidic chamber
• to evaluate targeting , motility , velocity of bacteriobot
• This device composed of 3 chambers :
Normal cells - Bacteriobots - tumor cells
• Uses fluorescent dyes to see Nano robots target cells
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flow-free chemotactic microfluidic chamber
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• Stage 3 : Tumor targeting and localization of bacteriobot
• It is the chemotactic response of bacteriobot to the [con] gradients of various of cancer cells :
• NIH/3T3 , CT_26 ( colorectal cancer )
• 4T1 ( breast cancer )
• Targeting measured by migration of bacteria out of central region toward the side chambers
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Results
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References
"New Paradigm for Tumor Theranostic Methodology Using Bacteria-based Microrobot."Nature.com. Nature Publishing Group, n.d. Web. 18
Feb. 2014.
Done by : Belal Abu Haneya
Hashemite University – Jordan
Supervisor : Dr. Abd Allatif Al Ghzawi