nanopore membranes for single-molecule detection and characterization anna schibel, ryuji kawano and...
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Nanopore Membranes for Single-Molecule Detection and Characterization
Anna Schibel, Ryuji Kawano and Henry S. White
University of Utah
October 26, 2007
Fabrication of the GNP Membrane
Zhang, B.; Galusha, J.; Wang, G.; Bergren, A. J.; Jones, R. M.; White, R. J.; Ervin, E. N.; Cauley, C. C.; Shiozawa, P.; White, H. S. Anal. Chem. 2007, 79, 4778-4787.
Bo Zhang
Nanodisk electrodeNanopore electrodeNanopore membrane
Stochastic Single-Molecule Detection
- hepta-sulfano--cyclodextrin (s7CD)
R = -OSO3-
Bound State
Unbound State
- -Hemolysin (HL)
HL insertionSingle Binding Events
Applied Voltage -40 mV
Gu e
t al., S
cience
20
01, 2
91, 6
36-6
40
1 M KCl10 mM Potassium phosphate bufferpH 7.43 M aHL backside50 M s7CD frontside
250 nm pore
Stochastic Single-Molecule Detection
R = NH3+
Clement Osei AkotoRainer Group
trans
cis - Reference
+ - a7CD
+
+ +
50 M a7CD
Voltage in wrt cis side50 M a7CD detection
Electrostatic trapping
Electrostatically trap molecule inside HL channel
1 si
a7CD
GNP radius - 95 nm
Nanoparticle Detection
Wang, G., Single Nanoparticle Sensing with Protein Ion Channel Embedded in Lipid Bilayer Supported By Glass Nanopore Membrane (unpublished)
Gold Nanoparticle with Tiopronin Monolayer
2nm
1 sec
9 bp-GC
1 sec
9 bp-TG
1 sec
9 bp-AT
~1 minute
Curr
ent
Discrimination Of Individual DNA Hairpin Molecules
Applications In Development
•Biomedical Analysis•DNA sequencing•Heparin analysis
•Homeland Defense•Chemical warfare agent detection
•Environmental Analysis•Ultra-trace level detection of pollutants•Nano-particle analysis