nanomaterials for drug delivery
DESCRIPTION
Group Meeting, 18th November, 2013 (Monday)TRANSCRIPT
U g e l s t a d L a b o r a t o r y
Nanomaterials for Drug Delivery
SulalitPhD StipendiatUgelstad Laboratory, Dept. of Chem. Eng., NTNU
18th November, 2013
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Roadmap
Drug pathway
Golden rules
Developments on Layer by layer (LBL) approach
Developments on hydrogels
Future Work
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E. Markovsky et al. / Journal of Controlled Release 161 (2012) 446–460
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Monodisperse Nanoparticle (NP) population
Biocompatibility
Long circulation times
Target specific
Delivery of cargo
Golden Rules
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LBL approach - outline
Ag
(a) (b)
Sodium citrate synthesis with reflux
Poly Lysine (PL) coating
Rat PGP siRNA and negative control siRNABBB
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Na-citrate baseline
PL addition
siRNA addition
∆f
(Hz)
Time (s)
∆mPL = 8533 ng cm-2
∆msiRNA = 2854 ng cm-2
Sauerbrey equation:
QCM Results
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LBL approach – preliminary results
(a) (b)
~38nm Ag NPs (DLS) , -45 mV
~66nm Ag-PL NPs (DLS) +33 mV
Ab
sorb
an
ce
Wavelength (nm)
(a) 427nm
(b) 432nm
- PGP siRNA- Ctrl siRNA
PGP silencing in vitro
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- Monomer + cross linking agent in water
-T>LCST
-Homogeneous nucleation on collapsed oligomers
-Oligomer and monomer addition, aggregation
Low PDI, control of charge,size, cross-link density
Precipitation polymerization
Sythesizing PNIPAm
Re-crystallization
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Size of PNIPAm at different concentrations
Size(nm)
Temperature (°C)
LCST
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PNIPAm concentration of 14.73 mg/mL
Size(nm)
Temperature (°C)
LCST
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Size of PNIPAm/AAc at different concentrations
Size(nm)
Temperature (°C)
VPTT
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Optimization of Au-PNIPAM-PAAC hydrogels
PGP silencing studies
EM setup for release studies
Drug Release Kinetics
Shape effect in coating
The road ahead!
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