na-mic national alliance for medical image computing measuring alcohol and stress interactions with...
TRANSCRIPT
NA-MICNational Alliance for Medical Image Computing http://na-mic.org
Measuring Alcohol and Stress Interactions with Structural and Perfusion MRI
Chris Wyatt
External Collaboration
National Alliance for Medical Image Computing http://na-mic.org
Introduction
• Our goal is to use MRI to examine the effects of chronic alcohol self-administration on brain structure and function in monkeys.
• Team– Jim Daunais, Bob Kraft (WFUHS)– Xiaoxing Li, Vidya Rajagopalan, Chris Wyatt (VT)
– Andriy Fedorov, Killian Pohl, Sandy Wells (NA-MIC)
National Alliance for Medical Image Computing http://na-mic.org
Experimental Design
• Male vervet monkeys were chosen based on their initial cortisol response to novelty.
• Classified as: – high cortisol response (HC)– low cortisol response (LC)
• Groups then divided into EtOH or controls
• Classic 2 X 2 design:HC/control LC/control
HC/EtOH LC/EtOH
National Alliance for Medical Image Computing http://na-mic.org
Experimental Protocol
(2 drinks) (4 drinks) (6 drinks)Baseline structuraland MRS
Post-induction structural, MRS and perfusion
Cynomolgus Monkey At The Panel (Courtesy Dr. Kathleen Grant, OHSU)
Custom Head Coil Example T1w Image
National Alliance for Medical Image Computing http://na-mic.org
NA-MIC Related Goal• Implement and validate algorithms for:
Size comparison of human, rhesus, and cynomolgus brain.
brain extraction
white-gray matter segmentation
subcortical structure segmentation
National Alliance for Medical Image Computing http://na-mic.org
Progress Report• Version 1 of Vervet atlas complete• Initial workflow implemented, documented
in a tutorial• Subcortical structures well segmented,
issues with image artifacts and cortical segmentation
• All data is collectedN = 10 subjects
National Alliance for Medical Image Computing http://na-mic.org
Current Efforts• Working on a Slicer only workflow to help
with dissemination– BRAINSFit Module replacement for FSL-flirt
• Working on an improved atlas • Segmenting second time point data• Analysis of results• Resubmission
– continue structural – add functional imaging
National Alliance for Medical Image Computing http://na-mic.org
Acknowledgements
Measuring Alcohol and Stress Interactions with Structural and Perfusion MRINIH NIAAAR01 AA016748-01 (PI James Daunais)
Early Stress & Alcoholism: Neurobiological AnalysisNIH NIAAAU01AA014106-07 (PI David Friedman)
Team–Jim Daunais, Bob Kraft (WFUHS)–Xiaoxing Li, Vidya Rajagopalan, Chris Wyatt (VT)
–Andriy Fedorov, Killian Pohl, Sandy Wells (NA-MIC)