21st December 2008 UK NEQAS User DayMYCOLOGY

for Microbiology

Mycology Identification Scheme

Dermatophyte Specimens

21st December 2008 UK NEQAS User DayMYCOLOGY

Overview

• Review dermatophyte specimens distributed

• New specimen format development

• Results from pilot distribution

21st December 2008 UK NEQAS User DayMYCOLOGY

What are the design criteria for EQA schemes?

• Clinically relevant• Homogeneous specimens• No matrix effect• Stable specimens• Adequately characterised• Measurement and assessment of

performance is possible

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Developing new specimens

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Specimen options

• Lyophilisation

• Under mineral oil

• Drying on silica gel

• Soil storage

• Spore suspensions in water

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Preparation of spore suspensions

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21st December 2008 UK NEQAS User DayMYCOLOGY

Dermatophytes selected Trichophyton rubrum

Trichophyton tonsurans

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Dermatophytes selected Trichopyton interdigitale

Epidermophyton floccosum

21st December 2008 UK NEQAS User DayMYCOLOGY

21st December 2008 UK NEQAS User DayMYCOLOGY

21st December 2008 UK NEQAS User DayMYCOLOGY

21st December 2008 UK NEQAS User DayMYCOLOGY

21st December 2008 UK NEQAS User DayMYCOLOGY

21st December 2008 UK NEQAS User DayMYCOLOGY

21st December 2008 UK NEQAS User DayMYCOLOGY

Acknowledgements

Staff and participants ofUK NEQAS forMicrobiology

Nita PatelMalti nakraniAna Deheer GrahamMousoumi DaschowdhuryMartha Valencia

Special thanks to:StudentsAnita MarwahaKalana Patabendige

MycologyReference LaboratoryBristol