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Mutagenesis Cloning Multiplexing Sequenching Gene Expression Exploring PCR 2 Thermo Scientific ™ Phusion ™ Flash High-Fidelity PCR Master Mix features extension times of 15 s/kb or less. Fast PCR 5 Go straight from sample to DNA amplification. 3 RNA template cDNA PCR • Reverse transcriptases are the replicating enzymes of retroviruses • RT-PCR can happen in a 1-step assay or a 2-step assay Reverse transcription PCR 1 Quantitative PCR vs. Probe-based chemistries SYBR ™ technology–based chemistries • 84% of researchers doing qPCR use a hot-start DNA polymerase • 79.1% analyze qPCR data by the standard curve method • 44% use predeveloped assays 4 Thermo Scientific ™ Phusion ™ High-Fidelity DNA Polymerase is 52x more accurate than Taq polymerase. High-fidelity PCR 52x Taq Taq 1 polymerase error rate = Fidelity Direct PCR Taq Polymerase is named “Molecule of the Year” by Science. 1989 PCR published in Science. 1985 Mutagenesis Cloning Sequencing Genotyping Gene expression Pathogen detection Multiplexing Explore PCR applications Don’t forget to add Explore different types of PCR 10 9 copies of DNA are made after 30 cycles of PCR in 1 reaction D N A p o l y m e r a s e MgCl 2 D N A t e m p l a t e P R I M E R S Learn more at thermofisher.com/tsmolbio For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. COL010409 1119 d N T P s A G T C

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Page 1: Mutagenesis Cloning Exploring PCR Multiplexingt ec r Di PCR Taq Polymerase is named “Molecule of the Year” by Science. 1989 PCR published in Science. 1985 Mutagenesis Cloning Sequencing

Mutagenesis Cloning

MultiplexingSequenching

Gene Expression Exploring PCR

2Thermo Scientific™ Phusion™ Flash High-Fidelity PCR Master Mix features extension times of 15 s/kb or less.

Fast PCR

5 Go straight from sample to DNA amplification.

3RNA template

cDNA

PCR

• Reverse transcriptases are the replicating enzymes of retroviruses

• RT-PCR can happen in a 1-step assay or a 2-step assay

Reverse transcription PCR

1 Quantitative PCR

vs.

Probe-basedchemistries

SYBR™

technology–based chemistries

• 84% of researchers doing qPCR use a hot-start DNA polymerase

• 79.1% analyze qPCR data by the standard curve method

• 44% use predeveloped assays

4Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase is 52x more accurate than Taq polymerase.

High-fidelity PCR52x Taq

Taq1polymerase error rate

= Fidelity

Direct PCR

Taq Polymerase is named “Molecule of the Year” by Science.

1989

PCR published in Science.

1985

Mutagenesis

Cloning

Sequencing

Genotyping

Gene expression

Pathogen detection

Multiplexing

Explore PCR

applications

Don’t forget

to add

Explore different types of PCR

109 copies of DNA

are made after 30 cycles

of PCR in 1 reaction

DNA polymerase

MgCl2

DN

A tem

plate

PRIMERS

Learn more at thermofisher.com/tsmolbio

For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. COL010409 1119

d

N T Ps

AGTC

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