muscle tissue histology

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Smooth Smooth muscle muscle (HE) (HE) Skeletal Skeletal muscle muscle ( ( cross cross section section HE) HE) Skeletal Skeletal muscle muscle ( ( longitudinal longitudinal section section HE) HE) Cardiac Cardiac muscle muscle (HE) (HE) Cardiac Cardiac muscle muscle ( ( iron iron - - hematoxylin hematoxylin ) )

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Page 1: Muscle Tissue histology

SmoothSmooth musclemuscle (HE)(HE)

SkeletalSkeletal musclemuscle ((crosscross sectionsection HE)HE)

SkeletalSkeletal musclemuscle ((longitudinallongitudinal sectionsection HE)HE)

CardiacCardiac musclemuscle (HE)(HE)

CardiacCardiac musclemuscle ((ironiron--hematoxylinhematoxylin))

Page 2: Muscle Tissue histology

SmoothSmooth musclemuscle HM, HM, 40x 40x (HE)(HE)SmoothSmooth musclemuscle cellcell ((oneone nucleusnucleus, , thinthin, , bobbinbobbin shapeshape))The The cellscells arrangedarranged inin fasciculifasciculi..The The nucleusnucleus is is localisedlocalised inin thethe centre centre ofof thethe cellcell. . ThereThere areare no no striatedstriated

Smooth muscle nucleus

Page 3: Muscle Tissue histology

The The functionalfunctional unit unit ofof itit is is thethe muscularmuscular fibrefibreThereThere is a is a fusionfusion ofof thethe myoblastmyoblast cellscells duringduring thethe ontogenesisontogenesisThe The numbernumber ofof nucleinuclei maymay be more be more thanthan 100100

Page 4: Muscle Tissue histology

Epimysium

EndomysiumPerimysium

(Cohnheim field)

SkeletalSkeletal musclemuscle crosscross sectionsection, 20x (HE), 20x (HE)

Page 5: Muscle Tissue histology

SkeletalSkeletal musclemuscle crosscross sectionsection, 40x (HE), 40x (HE)

Page 6: Muscle Tissue histology

The The bandsbands, , whichwhich areare dyeddyed darkdark, show a , show a doubledouble refractionrefraction ofof lightlight. . ThatThat sswhywhy thesethese areare AnisotropesAnisotropes (A)(A)The The bandsbands, , whichwhich areare dyeddyed lightlight, , havehave singlesingle refractionrefraction ofof lightlight IsotropesIsotropes (I)(I)ThereThere is a is a thinthin lineline inin thethe middlemiddle ofof thethe II--bandband, , whichwhich is is calledcalled ZZ--bandbandThereThere is a is a lighterlighter lineline inin thethe middlemiddle ofof thethe AA--bandband, , whichwhich is is calledcalled HH--bandbandandand int he int he middlemiddle ofof thisthis lighterlighter lineline therethere is a is a darkdark lineline, , thethe MM--lineline..The The II--bandband becomesbecomes shortershorter whenwhen thethe musclemuscle contractscontracts

ThoseThose contractilecontractile elementselements whichwhich show show striatedstriated patternpattern is is calledcalledmyofibrilsmyofibrils..The The placeplace whichwhich is is betweenbetween twotwo ZZ--bandsbands isis calledcalled sarcomersarcomerThe The myofibrillummyofibrillum consistsconsists ofof myofilamentsmyofilaments..

thinthin (9 (9 nmnm actinactin))

thickthick (12(12--15 15 nmnm myosinmyosin))

Page 7: Muscle Tissue histology
Page 8: Muscle Tissue histology

HarHaráántcsntcsííkolt izom HM, 40x (HE)kolt izom HM, 40x (HE)

Muscle cell nuclei

Striated pattern

Fibrocyte nucleus

SkeletalSkeletal musclemuscle ((longitudinallongitudinalsectionsection, 40x (HE), 40x (HE)

Page 9: Muscle Tissue histology

CardiacCardiac musclemuscle HE 60xHE 60xSingleSingle muslemusle cellscells, , therethere areare cellcell connectingconnecting structuresstructures atat thethe endend ofof themthemThreeThree otherother cardiaccardiac muslemusle cellcell cancan attachattach toto oneone cardiaccardiac musclemuscle cellcell ((YY--shapeshape))EberthEberth ss-- lineslines, , striatedstriated patternpattern

Page 10: Muscle Tissue histology

WeWe differenciatedifferenciatebetweenbetween twotwo part part lineslines becausebecause ofof thetheterracedterraced structurestructure

TransversalTransversal partpart: : fasciafascia adherensadherensdesmosomesdesmosomes

LongitudinalLongitudinal partpart::nexus (nexus (gapgap--junctionjunction))

theythey areare responsibleresponsibleforfor ionicionic traffictraffic

Cardiac muscle EM 29500x

Page 11: Muscle Tissue histology

Eberth s-line

nucleus

CardiacCardiac musclemuscle 60x60x