mosquito monitoring at crooked horn farm€¦ · 12/10/2018  · mosquito monitoring at crooked...

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Project Goals: Assess the ratio of mosquito to natural enemies within the aquatic invertebrate population in local wetlands using Canadian Aquatic Biomonitoring Methods (CABIN) Verify CABIN results and increase search effort by quick dip sampling Encouraging wetland stewardship and restoration Mosquito monitoring at Crooked Horn Farm Darcie Quamme 1,5 , Rhia MacKenzie 2,5 , Gregoire Lamoureux 2,5 , Richard Johnson 3,5 , & Ryan Durand 4 ,5 If you have a backyard wetland and want to be part of an innovative study please contact: Darcie Quamme, Integrated Ecological Research, [email protected] , or Rhia MacKenzie, Slocan River Streamkeepers, [email protected] , full report at slocanswamp.org What are macroinvertebrates? Organisms without a backbone Visible to the naked eye Variable tolerances to stressors The suite of invertebrates indicates health Mosquitoes are one type of macroinvertebrate Conclusions: Mosquitoes (n=51 kick net samples) comprised 0-6% of all invertebrates in emergent vegetation (2014-18) at all sites Mosquitoes comprised <2% of all invertebrates in 2016-18 at Crooked Horn Farm in pre and post-restoration monitoring (Kick-net, n=1 per year) Post-restoration monitoring in 2017 using 350mL dip sampler found zero larvae in 18 samples within the wetland & none in ditches (6 samples). Post-restoration monitoring in 2018 using 350mL dip sampler found two larvae in 24 samples within the wetland & none in ditches (6 samples). Methods: Parameters monitored included: Mosquitoes from emergent vegetation Water & sediment chemistry % Composition of emergent vegetation Habitat variables & stressors Indicators Site locations Mosquitoes at all sites were in the genus Anopheles Results: % Mosquitoes in constructed vs reference sites at Floodplain sites (n=26) % of invertebrates that were of the family Culidae in lower Slocan Valley sedge/cattail wetland habitat for constructed versus reference site Methods will be used to track restoration goals 1 Integrated Ecological Research, 2 Slocan River Streamkeeper Society, 3 Opus Petroleum Eng., 4 EcoLogic Consulting, 5 Slocan Valley Wetland Assessment & Monitoring Program Collection: Kick-net: 3 minute kick sample Standard area (25m 2 ) Larval dip sampler (350 mL) Emergent Plants Water, Sediment, Physical, GIS Wetland Stress Vegetation, Invertebrates Biological health Further analyses to be carried out: Calculation of predator : mosquito ratios Correlations with environmental variables such as degree days, and water level indices Larval dip sampler Kick-net sampling Meta-data Wetland restoration Examining Kick-net sample Dragonflies, adult & larvae: natural enemies Dip sampling Mosquito larvae Inspecting the site 0 1 2 3 4 5 6 7 8 9 10 SPA001_16_Pre SPA001_17_Post SPA001_18_Post MC003_16_Post MC003_17_Post MC003_18_Post MC002_16_Post MC002_17_Post MC002_18_Post MC001_16_Post MC001_17_Post MC001_18_Post HAY001_15 FRA001_15 SCH001-15 TY001_15 FO001_15 ELD001_15 MC004_17 MC004_18 MC005_17 MC005_18 MC006_17 MC006_18 OUT001-17 OUT001-18 Percent Composition %Culicidae in Floodplain wetlands Constructed wetlands Reference wetlands

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Page 1: Mosquito monitoring at Crooked Horn Farm€¦ · 12/10/2018  · Mosquito monitoring at Crooked Horn Farm Darcie Quamme 1,5 , Rhia MacKenzie 2,5 , Gregoire Lamoureux 2,5 , Richard

Project Goals:• Assess the ratio of mosquito to natural enemies

within the aquatic invertebrate population in local wetlands using Canadian Aquatic Biomonitoring Methods (CABIN)

• Verify CABIN results and increase search effort by quick dip sampling

Encouraging wetland stewardship and restoration

Mosquito monitoring at Crooked Horn Farm Darcie Quamme1,5, Rhia MacKenzie2,5, Gregoire Lamoureux2,5, Richard Johnson3,5, & Ryan Durand4 ,5

If you have a backyard wetland and want to be part of an innovative study please contact: Darcie Quamme, Integrated Ecological Research, [email protected], or Rhia MacKenzie, Slocan River Streamkeepers, [email protected] , full report at slocanswamp.org

What are macroinvertebrates?• Organisms without a backbone• Visible to the naked eye• Variable tolerances to stressors• The suite of invertebrates indicates health• Mosquitoes are one type of macroinvertebrate

Conclusions:• Mosquitoes (n=51 kick net samples) comprised 0-6% of all invertebrates in

emergent vegetation (2014-18) at all sites• Mosquitoes comprised <2% of all invertebrates in 2016-18 at Crooked Horn

Farm in pre and post-restoration monitoring (Kick-net, n=1 per year)• Post-restoration monitoring in 2017 using 350mL dip sampler found zero

larvae in 18 samples within the wetland & none in ditches (6 samples).• Post-restoration monitoring in 2018 using 350mL dip sampler found two

larvae in 24 samples within the wetland & none in ditches (6 samples).

Methods:Parameters monitored included:• Mosquitoes from emergent vegetation• Water & sediment chemistry • % Composition of emergent vegetation• Habitat variables & stressors

Indicators

Site locations

Mosquitoes at all sites were in the genus Anopheles

Results:

% Mosquitoes in constructed vs reference sites at Floodplain sites (n=26)

% of invertebrates that were of the family Culidae in lower Slocan Valleysedge/cattail wetland habitat for constructed versus reference site

Methods will be used to track restoration goals

1Integrated Ecological Research, 2Slocan River Streamkeeper Society, 3Opus Petroleum Eng., 4EcoLogic Consulting, 5Slocan Valley Wetland Assessment & Monitoring Program

Collection:• Kick-net: 3 minute

kick sample• Standard area (25m2)

• Larval dip sampler(350 mL)

Emergent Plants

Water, Sediment,

Physical, GIS

Wetland Stress

Vegetation, Invertebrates

Biological health

Further analyses to be carried out:• Calculation of predator : mosquito ratios• Correlations with environmental variables such as

degree days, and water level indices

Larval dip samplerKick-net sampling

Meta-data

Wetland restoration

Examining Kick-net sampleDragonflies, adult & larvae:natural enemies

Dip sampling

Mosquito larvae

Inspecting the site

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%Culicidae in Floodplain wetlands

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Reference wetlands