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Mosaic Digital Illumination

Specifications Summary

Transmission 360 nm to 800 nm

Extinction ratio > 1000:1

Minimum resolvable spot Diffraction limited with 60x or 100x objective

Optical pixel rise / fall time < 1 µs

Minimum optical pulse width75 µs external trigger100 µs internal trigger

Maximum frame repition rate 600 frames / sec

Certification CDRH IIIb (if fitted with a laser source)

Features and Benefits • Unlimited flexibility in shape, size and

complexity of illumination mask

• Simultaneous illumination of multiple

regions of interest

• Precise illumination of areas of interest

that protects target specimen and

fluorophore

• Zero delta acquisition time for true

digital excitation/illumination

• Longest lifetime and lowest

maintenance with rugged

semiconductor device

Mosaic Real Time Parallel Illumination of Multiple RegionsMosaic is a patented instrument platform built around MEMS Digital Mirror Devices (DMD). Mosaic

exploits DMD in a proprietary programmable platform, integrated with scientific light sources

including lasers, LEDs and arc lamps, and operates from 360 to 800 nm. It is offered with a range

of high performance microscope adapter optics and can be integrated with CLSM, spinning disk

and wide field imaging modalities. High speed frame switching (60 Hz) makes Mosaic suitable for

many dynamic applications including bleaching, uncaging, photo-switching, optogenetics and

constrained illumination. Variable intensity distributions can be achieved by rapid gating of mirror

patterns.

DMD TechnologyThe core of Mosaic is the Digital

Micromirror Device (DMD), a high speed

and highly efficient semiconductor-based “light

switch” array of hundreds of thousands

hinge-mounted, addressable, tiltable,

microscopic mirrors.

When a DMD chip is coordinated with a digital

video or graphic signal, a light source, and

beam delivery optics, its mirrors reflect a

digital image of the illumination mask onto the

sample.

Active Illum

ination



Illumination Source Options

DMD illumination ports 1 or 2 on left hand side* 1 or 2 on left hand side, 1 or 2 on right hand side*

Incoherent sources High pressure mercury lamp (100 W - 300W)DG4

Andor AMH-200Other light sources on request

CW laser 405 nm diode488 nm Argon514 nm Argon

Other on request

* For 7201 / 7204 / 7205 allow only 1 port, 7206 allows 2 portsAdditional illumination source options available on request

Hardware SpecificationsOptical

Model Mosaic Mosaic Duet

Transmission 360 nm to 800 nm

Intensity stability Absolute

Extinction ratio > 1000:1

Minimum resolvable spot Diffraction limited with 100x objective

Fields of view 7206 = 5.28 x 3.96 mm 7201 = 8.64 x 6.48 mm

7204 and 7205 = 13.12 x 9.84 mm

Illumination port clear aperture Ø 34 mm

For flat field illumination port options, please see table at bottom of this secion

Illumination Port Options

Dichroic beamsplitter Single pass, specify wavelengthMulti-pass, specify wavelength

Beamsplitter 360 nm to 700 nm, R = 100, 70, 50 or 30%

Excitation filter * 480 nm / 20 nm (GFP), 470 nm / 40 nm (FITC) - 38 mmOther filters on request - 25 mm with adapter

Laser safety filter, interlocks Laser specific

Shutter Optional

* Additional excitation filter options available on request

INTEROPERABILITYCompatible with microscopes platforms - point & slit scan confocal, spinning disk confocal & widefield.

Custom OEM systems available for High Content Screening and other fluorescence based imaging instrumentation.

Compatible with control and acquisition software from market leading microscope manufacturers including Carl Zeiss,

Leica and Olympus.

Wavelength modularity - add lasers and filters cost effectively.

Flat field Epi illumination ports

Adapter Leica DMI series Nikon TE2000 Nikon Ti series Nikon Universal Olympus IX series

Part Number MM-7310-DMI-FFEP-X* MM-7310-TE2K-FFEP-X* MM-7310-TI-FFEP-X* MM-7310-NFS-FFEP-X* MM-7310-IX70-FFEP-X*

Adapter Zeiss Axiovert 200 Zeiss Axio Examiner D1 Zeiss Axio Imager M1 Zeiss Axio Observer C-mount video port

Part Number MM-7310-ZSAV-FFEP-X* MM-7310-D1-FFEP-X* MM-7310-IMG-FFEP-X* MM-7310-OBS-FFEP-X* On request

*For 7201 / 7204 / 7205, X = 1*For 7206, X = 2



Software SpecificationsThe Mosaic system includes software that controls calibration and enables the user to graphically or numerically define and edit the shape and

characteristics of the illumination at the target.

Release MicroPoint Mosaic Version 1.1

Drivers PLX 9054 API version 4.1 for Mosaic.exe UIPLX 9054 API version 6 for SDK

Calibration Scale & Offset corrections for X & Y8 user-defined descriptions

Operating systems Windows 2000, XP

File Functions Import (*.dxf, *.bmp, *.msk); Save *.msk

Drawing Editor Shapes Line, Paintbrush, Rectangle, Oval, Freehand polyshape

Drawn Shape Characteristics Linewidth, paintbrush size, Alpha blended color and opacity of defined regions, Color palletteClick and drag shapes and edges, ut, copy, paste, delete shape, Group, ungroup shapes

Controls Engage mask pattern, Go dark (global), Stop, Snapshot video

Options Continuous update from drawing editor, Configure video subsystem, Exposure time & trigger selectionVideo mode & time selection, On/Off, Adjust laser outline highlight

The angular alignment of either the CW laser or lamp illumination is factory set. The spatial alignment is controlled via the software calibration routine. The

focus in the z direction is fixed at installation or OEM integration. Beam shaping is then controlled with the spatial light modulating DMD.

Define region of interest and excitation

energy at the target in image acquisition

software

Simultaneously excite and acquire image

with zero delta time

Software Compatibility - Life Science ApplicationsMosaic systems are compatible with a wide range of life science imaging software as indicated in the table below.

Andor iQ Version 2.0 Live cell multi-dimensional imaging with fexible control of MicropPoint

MicroPoint Laser Galvo Version 1.0 Image-based targeting, control & calibration

MDC MetaMorph Ver 7.1 and above (32-bit) Fully integrated MicroPoint support with powerful imaging capabilities

MDC MetaMorph Ver 7.74 and above (64-bit) Fully integrated MicroPoint support with powerful imaging capabilities

Nikon NIS Elements 3.0 SP4 Build 505 Multi-dimensional imaging with simple/fast control of MicroPoint

Software Development Kits (SDK) SDKs are available for OEM to develop software to control integrated MicroPoint systems



ComponentsThe main components of MicroPoint are shown below:

Flange for EPI source

DMD array

Flange for optional second light source

Beamsplitter/ dichroic plugs

Laser zoom collimatorLaser fiber input

Electronic interface



Ordering your Mosaic system

Prior to commencing the order process please advise your customer representative of your application requirements. Please also refer to the flow charts on the next 2 pages.

In order to allow us to select the correct model for your requirements please advise your customer representative of the make, model (and any accessories fitted) of the microscope being used.

Step 1.

Please select the field of illumination required.

Step 2.

Choose one or more EPI / Mosaic beamspliiters.

Step 6.

Select the type of EPI light source require.

Step 4.

Select the primary left sided DMD light source. For the 7206 option only, a secondary left side light source can also be selected at this point.

Step 3a.

For Mosaic Duet systems only a primary right sided light source can also be selected. For the 7206 option only, a secondary right side light source can also be selected at this point

Step 3b.

Step 1.

Confirm your Microscope make and modelLeica, Nikon, Olympus, Zeiss

Step 2.

The following field of ilumination options (diagonal) are available:

7201 11.5 mm; 7204 17.4 mm; 7205 17 mm Axio Tirf; 7206 7 mm

Choose one or more optional EPI excitation filters.

Step 5.

Step 5.

Optional EPI Excitation filters are also available:

GFP filter in holder (480/20), FITC (470/40), Others on request

Step 4.

The following EPI light sources are available:

Andor AMH, DG4, Microscope specific Hg lamp, MicroPoint, other

Step 3a.

The following DMD light sources / filter sets for the left side port of Mosaic are available:

Diode laser, Andor ALC, Other laser, Andor AMH, DG4, Hg

The following left sided excitation filters are available:

GFP filter in holder (480/20), FITC (470/40) ,Blank filter holder, Others on request

Secondary left sided DMD source (7206 only), select one or more of the following

beamsplitters:

30 Epi, 50 Epi,70 Epi, 100% Epi Plug; 0% Epi (Blank) Plug, A range of dichroic mirrors is

available on request.

Step 6.

The following Epi-Mosaic beamsplitters are available:

30 EPI, 50 EPi, 70 EPI, 100% Epi plug, 0% Epi (Blank) Plug

Step 3b.

For Mosaic Duet systems only, additional DMD ight sources / filter sets for the right side port:

Diode laser, Andor ALC, Other laser, Andor AMH, DG4, Hg

The following right sided excitation filters are available:

GFP filter in holder (480/20), FITC (470/40) ,Blank filter holder, Others on request

Secondary right sided DMD source (7206 only), select one or more of the following

beamsplitters:

30 Epi, 50 Epi,70 Epi, 100% Epi Plug; 0% Epi (Blank) Plug, A range of dichroic mirrors is

available on request.

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0

Shutter Moves 38.1[1.50]

Removeable Beamsplitter57 [2.2] Ø Cylinder

Duet

76

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3.0

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80 [3.1]

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(Laser Input Attachment)

160 [6.3]240 [9.375]

(Lamphouse Attachment Plane)

Mosaic Lamphouse Adapter

Duet

76

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160 [6.3]

333 [13.1]

229 [9.0]

146 [5.8]+ 100 [4] Fiber Bend Radius

Laser Fiber Collimator

Shutter

Duet

Shutter

Weights:

Mosaic Head = 3.5 kg [7.5 lb]

Mosiac Duet Head = 4.5 kg [9.8 lb]

Product DrawingsDimensions in mm [inches]

7206

7201 - 7205

Head Dimensions

Model Part Number Length

7201 MEC-04686 369.06 mm [14.53]

7204 MEC-04685 385.57 mm [15.18]

7205 MEC-04684 356.06 mm [14.03]

7206 MEC-04729 333.00 mm [13.1]

Fields of Illumination (FoI)

Model 7201 7204 7205 7206

FOV (Lamp house)Diagonal, mm 11.54 17.44 17.44 7.12

FOV (Lamp house)XY, mm 9.23 x 6.92 13.95 x 10.46 13.95 x 10.46 4.88 x 3.66

FOV 0.4 mm fiber ZC* x2 (mm)** 8.40 X 6.92 11.90 X 10.46 11.90 X 10.46 4.2 X 3.66

FOV 0.4 mm fiber ZC x1 (mm)*** 4.4 6.7 6.7 2.7

FOV 0.2 mm fiber ZC x2 (mm)** 4.0 6.1 6.1 2.5

FOV 0.2 mm fiber ZC x2 (mm)** 2.2 3.4 3.4 1.4

DMD pixel size (µm) 12 17 17 6

*ZC = Zoom Collimator X2 = max; X1 = min ** Intersection of circle and DMD rectangle*** Circular field of illumination

Shutter

Duet

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Removeable Beamsplitter57 [2.2] Ø Cylinder

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See Table

14

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97 [3.8]

175 [6.88](Lamphouse Attachment Plane)

Mosaic Lamphouse Adapter

Laser Fiber Collimator

Shutter



Order TodayNeed more information? At Andor we are committed to finding the correct solution for you. With a dedicated team of technical advisors, we are able to offer you one-to-one guidance and technical support on all Andor products. For a full listing of our regional sales offices, please see:

Our regional headquarters are:Europe Japan

Belfast, Northern Ireland Tokyo

Phone +44 (28) 9023 7126 Phone +81 (3) 3518 6488

Fax +44 (28) 9031 0792 Fax +81 (3) 3518 6489

North America China

Connecticut, USA Beijing

Phone +1 (860) 290 9211 Phone +86 (10) 5129 4977

Fax +1 (860) 290 9566 Fax +86 (10) 6445 5401

www.andor.com/contact

MMosaicSS 0411 R2

Minimum Computer Requirements:

The imaging computer must have two adjacent PCI

slots (with exceptions) and a bay for BNC Trigger

connectors. Application software for the Mosaic

Digital Diaphragm System is typically integrated into

the imaging software provided for the microscope.

Operating & Storage Conditions

Operating Temperature 0°C to 30°C ambient

Relative Humidity < 70% (non-condensing)

Storage Temperature -25°C to 50°C

NOTE: Laser radiation is only present in Mosaic systems fitted with a laser. Some Mosaic systems use non laser-based light sources.

1x DMD Head assembly, Mosaic II

1x PLX and daughter board pair, programmed

1x 10’ SCSI-3 Ultra LVD/SE MD698 cable

1x TPES15-T050PP-7 Mosaic custom power supply

1x 3m mains cable for TPES15-T050PP-7

1x First surface mirror

10x Green Post-It notes

1x Allen key (0.05”)

1x T-handle (3/32)

1x LED Maglite, with 2 xAA batteries

1x Maglite clamp

1x Mosaic post holder

1x Mosaic foot

1x NT-59-000 4” stainless steel post (1/4 - 20 UNC)

1x NT-58-999 2” stainless steel post (1/4 - 20 UNC)

1x Zeiss male flange

1x BNC finger assembly

1x User guide

Items shipped with your Mosaic:

Windows is a registered trademark of Microsoft Corporation.Power Requirements

100 - 240 VAC, 50-60 Hz, 1.0 A

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