August2009

New Products for August 2009New IL-22 antibodyThe Role of Basophils in the Th2 ResponseMore Colors, Better Flow ExperimentsLegendary Stem Cell Discovery

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BioLegend11080 Roselle St.

San Diego, CA 92121Tel: 858-455-9588, Toll-free: 877-246-5343, Fax: 858-455-9587

www.biolegend.com

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New Products For August 2009

Meet Team BioLegend!

Amy LeeAccount Manager,

Hiker and Rock climber

Jen BoyleAccount Manager,

New Jersey lover and Baker

Human Clone ConjugateAnti-human CD7 Antibody CD7-6B7 PEAnti-human CD34 Antibody 561 FITC, PE, PurifiedAnti-human CD34 Antibody 581 FITC, PE, PurifiedAnti-human CD38 Antibody HIT2 Alexa Fluor® 700Anti-human CD49c (integrin α3) Antibody ASC-1 Alexa Fluor® 488, PE, PurifiedAnti-human CD49e Antibody NKI-SAM-1 APCAnti-human CD166 Antibody 3A6 PE, PurifiedAnti-human CLEC5A Antibody 3E12C1 PE, PurifiedAnti-human Granzyme B Antibody GB11 Alexa Fluor® 647, FITCAnti-human IL-22 Antibody BG/IL22 PEAnti-human TCR Vα24-Jα18 (iNKT Cell) Antibody 6B11 APC, FITC

Mouse Clone ConjugateAnti-mouse CD3 Antibody 17A2 PE/Cy7Anti-mouse CD66a (CEACAM1a) Antibody MAb-CC1 Biotin

Anti-mouse CD146 Antibody ME-9F1 FITC, PE, Purified

Anti-mouse CD272 (BTLA) Antibody 8F4 PE, PurifiedAnti-mouse Ly-6C Antibody HK1.4 APC, Pacific Blue™, PE/Cy7Anti-mouse TCR γ/δ Antibody GL3 APC

Rat Products Clone ConjugateAnti-rat CD200 Antibody OX-2 PE, Purified

Cell Biology Clone ConjugateAnti-eIF3D (p66) Antibody 8A9 PurifiedAnti-eIF3H (p40) Antibody 11H5 PurifiedAnti-eIF3I (p36) Antibody 17C11 PurifiedAnti-Flightless-1 Antibody 6F7-FL PurifiedAnti-RANTES (CCL5) Antibody VL1 Purified

Recombinant Proteins Clone ConjugateRecombinant Mouse IL-10 (ELISA Std.)Recombinant Mouse VEGF120

Isotype Ctrl Clone ConjugateSyrian Hamster IgG Isotype Ctrl Antibody SHG-1 APC/Cy7

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New IL-22 antibody for intracellular flow cytometry!

ELISA

Recombinant Proteins

LEGEND MAX™ Human IL-22 ELISA Kit,1 pre-coated plate, 5 pre-coated plates

434507/8

Human ELISA MAX™ Deluxe kits, 5 plates, 10 plates, 20 plates

434504/5/6

Recombinant Human IL-22 (carrier free)

571302/4/6/8

Recombinant Mouse IL-22 (carrier free)

576202/4/6/8

Read more:Trifari, et al. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from TH-17, TH1 and TH2 cells. Nature Immunology. 2009. 10:864. Pubmed

Figure: PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were intracellular stained with PE IL-22 and APC CD4, clone RPA-T4 (left) and blocked with blocked with BioLegend recombinant human IL-22 (right)

View interactive pathways online at www.biolegend.comTh1 PathwayTh2 PathwayTh17 Pathway

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New Role for Basophils in Activation of Th2 responses

Perrigoue, J. et al. MHC class II–dependent basophil–CD4+ T cell interactions promote TH2 cytokine–dependent immunity. 2009. Nature Immunology. 10(7):697-705.

Yoshimoto, T. et al. Basophils contribute to TH2-IgE responses in vivo via IL-4 production and presentation of peptide–MHC class II complexes to CD4+ T cells. 2009. Nature Immunology. 10(7):706-12.

Th1-Th2 Flow kitsFlow kit Catalog #

Human Th1-Th2 Cytokine Flow Panel (FITC IL-2, FITC IFNg, PE IL-4, PE IL-10)

336501

Mouse Th1-Th2 Cytokine Flow Panel (FITC IL-2, FITC IFNg, PE IL-4, PE IL-10)

131101

Sokol, C.L. et al. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. 2009. Nature Immunology. 10(7):713-20.

Wynn, T.A. Basophils trump dendritic cells as APCs for TH2 responses. 2009. Nature Immunology. 10(7):679-81.

Read more:

T helper type 2 (Th2) mediated immune responses are induced by parasitic infection and allergic reaction. New research shows basophils trigger allergen and helminth driven Th2 responses by functioning as antigen-presenting cells.

View all Cell Markers here!

Antibodies for flow cytometry

CD123

CD203c (E-NPP3)

CCR3

TLR4

anti-human MHC II

anti-mouse MHC II

anti-human IgE

anti-mouse IgE

Antibodies view all formats at www.biolegend.com

Human basophilic leukemia cell line KU-812 stained with PE anti-human CD203c, clone NP4D6 (324605)

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More Color Options, Better Flow Experiments

100+ antibodies available in Pacific Blue™! including CD3, CD19, CD150, ckit, foxp3 and more.

60+ antibodies available in Alexa Fluor® 700! including CD4, CD14, IFNg, IL-17A and more.

More Fluorochrome Options: including Pacific Blue™, PerCP, PerCP/Cy5.5 and more

Tools for Multicolor Flow Cytometry

Fluorochrome/Flow Cytometer chartFluorochrome brightness indexExpression of common cell surface proteinsMulticolor Staining Guide WheelView our Resource Guides and ProtocolsFluorochrome Excitation/Emission ChartDownload our Interactive Human and Mouse CD Molecule ChartsFlow Cytometry Resources

Visit biolegend.com for interactive website tools.

Fluorochrome

APC

APC/Cy7

Alexa Fluor® 488 - Over 200 products!

Alexa Fluor® 647 – Over 300 products!

Alexa Fluor® 700 – Over 60 products!

FITC

PE

PE/Cy5

PE/Cy7

PerCP

PerCP/Cy5.5 – Over 150 Products

Pacific Blue™ - Over 100 products!

C57BL/6 splenocytes surface stained with CD4 (GK1.5) PE and then intracellularly stained with Pacific Blue™ anti-mouse Foxp3 (126409, clone MF-14)

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Legendary Stem Cell Discovery with

World Class Antibodies & Immunoassays

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Customer Testimonials

I would like to thank you for the pleasurable experience I encountered with you and your company. Not many businesses would work diligently to solve a customer’s problems, but Biolegend did exactly that. I am confident that Biolegend will always produce top notch antibodies, and if a rare problem does arise, I now know I can trust Biolegend to solve it. This experience will definitely make me come back to Biolegend for their antibodies (actually, you’ll be receiving an order soon from my lab).

Once again, thank you!

Sincerely,Michael

Michael W. CalikNeuroscience Doctoral CandidateNeuroscience InstituteLoyola University Chicago Stritch School of Medicine

“I am extremely impressed with the quality, amounts, price, and speed antibodies are shipped to us. I have been a regular customer for a number of years and plan to continue. Thanks.”

“We have found your abs to be superior to competitors in many cases. You have become our preferred supplier.”

Want to Travel to a Conference?The BioLegend Travel Award is a $500 prize towards a scientific meeting of your choice.

Enter today by emailing promos@biolegend.com

For graduate students, post-docs, and junior faculty. US customers only. For details and rules, check out: http://www.biolegend.com/travel_awards/

Hear what BioLegend Customers are Saying!

Would you like to give us feedback?contact BioLegend attechserv@biolegend.com

“Those antibodies are fantastic- they stained much higher than we got used to see with other companies’ ones. I already ordered and got more.”-Medical College of Georgia

“thank you very much for all the samples BioLegend has sent me. Without these, I wouldn’t be able to establish my multicolor combinations. Really appreciated. In fact Biolegend was the only company that offered me free samples. Moreover, the quality of the antibodies is just great. I used to work with Pharmingen and these are comparable. I will order all antibodies that I need from Biolegend.“-UCLA

“I would like to thank BioLegend and its president for giving us the opportunity to test a free sample of PD-1-PE. The antibody worked fine and is the best we could find so far: this is why we have submitted an order that was shipped today.”-National Immune Monitoring Laboratory, Canada

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August2009

BioLegend Inc.11080 Roselle St.San Diego, CA 92121Tel: 1-858-455-9588Tel: US Toll-Free: 1-877-Bio-Legend (1-877-246-5343)Fax: 858.455.9587Email: info@biolegend.comwww.biolegend.com

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