CignalTM Lenti ReportersMonitor Signaling Pathway Activity in Any CellCignalTM Lenti Reporters

Cignal Lenti Reporters

The Cignal Lenti Reporters are ready-to-transduce lentiviral particles for assessing cell signaling activities in virtually any mammalian cell type. This system utilizes a unique combination of robust transcription factor reporter technology and superb lentiviral delivery power. Applications Study Signaling Pathway Activities in Impossible to Transfect Cell Types Immune Cells Stem Cells Neuronal Cells Differentiated Cells Primary Cells Other Difficult-to-Transfect Cells

Transient Transduction Studies to Study the Biological Impact of: Drug candidates or chemical compounds Gene knockdown (siRNA, shRNA) or gene overexpression events Peptides, proteins, or ligands

Generate Pathway Sensor Cell Lines Valuable cell-based assay platform for drug or gene function studies Why Cignal Lenti Reporters? Ready-to-transduce lentiviral particles

Reporter technology exhibiting exceptional sensitivity, specificity, and signal-to-noise ratio

Available with luciferase or GFP as reporter gene

How Cignal Lenti Reporters Work

tel: 301.682.9200 fax: 301.682.7300

Cignal LentiReporter

live lentivirusparticles

TransientTransduction Studies

Transduction w/ Cignal Lenti

AssayReadout

Treatment

Plate Cells

AntibioticSelection

Establish PathwaySensor Cell Lines

Clonal CellLine Isolation

Transductionw/ Cignal Lenti

Plate Cells

High Efficiency Lentiviral Delivery Made These Studies Possible.

PRIMARY CELL STUDIES: NFAT Lenti Reporter (luciferase) Measures PKC/Ca++ Pathway Signaling Activity in Primary Arteriole Smooth Muscle Cells9

Relative Luciferase Units

3

10

Cignal Lenti NFAT Reporter(luciferase) + DMSO

654

Cignal Lenti Reporter (luciferase)+ 10 ng/mL PMA + 0.5 mM Ionomycin

2

78

DIFFICULT TO TRANSFECT CELL LINES: RBP-Jk Lenti Reporter (luciferase) Measures Notch Signaling

Pathway Activity in Neuronal Cells (C6 Rat Glioma Cells)

90

Relative Luciferase Units

30

100

Cignal Lenti RBP-Jk Reporter [10 MOI]+ Cignal Lenti Renilla Control (luciferase) [1 MOI]

+ 100 MOI Ad-GFP

605040

Cignal Lenti RBP-Jk Reporter [10 MOI]+ Cignal Lenti Renilla Control (luciferase) [1 MOI]

+ 100 MOI Ad-NIC

20

7080

100

Cignal Lenti Reporters 16 Cignal Lenti Reporters are Available!

* Each tube contains 250 �L of lentiviral particles (~ 2.5 x 106 transducing units). The Cignal Lenti Reporters are safe replication imcompetent, HIV-based, VSV-G pseudotyped lentiviral particles ©2008 SABiosciences Corporation All Rights Reserved. Cignal™ & Cignal Finder™ are trademarks of SABiosciences Corporation.

Cignal Lenti Reporter System:Luc Cat #

C/EBP C/EBPcAMP/PKA CREBCell Cycle E2F/DP1p53/DNA Damage p53Hypoxia Hypoxia-inducible Factor-1 (HIF-1)Type I Interferon STAT1/STAT2Interferon Gamma STAT1/STAT1MAPK/ERK Elk-1/SRFMAPK/JNK AP-1c-myc Myc/MaxNF�B NF�BNotch RBP-JkPKC/Ca++ NFATRetinoic Acid Receptor Retinoic Acid Receptor (RAR)TGF� SMAD2/SMAD3/SMAD4Wnt TCF/LEF

CLS-001LCLS-002L CLS-003LCLS-004LCLS-007LCLS-008LCLS-009LCLS-010LCLS-011LCLS-012LCLS-013LCLS-014LCLS-015LCLS-016LCLS-017LCLS-018L

CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844

CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874

Pathway Transcription Factor

6951 Executive Way, Frederick, MD 21703 USAwww.SABiosciences.com Support@SABiosciences.com

CLS-002G

CLS-010GCLS-011G

CLS-013GCLS-014G

CLS-018G

Cigna

l Len

ti Path

way R

eport

ers

Pack Size* PriceProduct Catalog #

Cigna

l Find

erTM

Lenti

Array

s Cignal Finder Lenti Cancer 10-Pathway Reporter ArrayCignal Finder Lenti Immune Response 10-Pathway Reporter ArrayCignal Finder Lenti Development 10-Pathway Reporter ArrayCignal Finder Lenti Toxicity 10-Pathway Reporter Array

CLA-001LCLA-002LCLA-003LCLA-004L

10 Tubes + Pos/Neg Controls10 Tubes + Pos/Neg Controls10 Tubes + Pos/Neg Controls10 Tubes + Pos/Neg Controls

CHF 2999CHF 2999CHF 2999CHF 2999

1-Tube Pack* 8-Tube PackProduct Catalog #

Cigna

l Len

ti Re

porte

r Con

trols

Cignal Lenti Positive Control (GFP)Cignal Lenti Negative Control (GFP)Cignal Lenti Positive Control (luciferase)Cignal Lenti Negative Control (luciferase)Cignal Lenti Renilla Control (luciferase)

CLS-PCGCLS-NCGCLS-PCLCLS-NCLCLS-RCL

CHF 844CHF 844CHF 844CHF 844CHF 844

CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874

GFP Cat # 1-Tube Pack*8-Tube Pack

GFP REPORTER FORMAT: SRE Lenti Reporter (GFP) Measures MAPK/ERK

Pathway Activation in CV1 Fibroblasts

Fold Induction

1.5

0.50

Cignal Lenti SRE Reporter [50 MOI](Untreated)

32.52.0

Cignal Lenti SRE Reporter [50 MOI]+10% FBS + 10 ng/mL PMA

1

3.54

STABLE PATHWAY SENSOR CELL LINES: NF B Lenti Reporter (luciferase) Establishes aStable HEK293-NF B Pathway Sensor Cell Line

Relative Luciferase Units

60

20

0

120

100

80

40

140

160

180

P1w/o

TNF-�10 ng/mL

TNF-�

P5w/o

TNF-�10 ng/mL

TNF-�

P10w/o

TNF-�10 ng/mL

TNF-�

P15w/o

TNF-�10 ng/mL

TNF-�

Untreated Treated