molecular differences resulting from larval growth conditions in aedes aegypti david price disease...
TRANSCRIPT
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MOLECULAR DIFFERENCES RESULTING FROM LARVAL GROWTH CONDITIONS IN AEDES AEGYPTI
DAVID PRICEDISEASE VECTOR MOLECULAR BIOLOGY LABNMSUPI IMMO HANSEN
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Background
Aedes aegypti is an important disease vector
Relatively safe model for malaria Plasmodium gallinaceum
Existing Reference Genome
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Aedes/Dengue Situation
http://www.healthmap.org/dengue/index.php
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Small vs Large Mosquitoes
Larval conditions affect adult insect Crowding, food availability ->Changes in adult size, time to reach
adulthood, immunity, reproductive success, energy stores, life span, fecundity on first blood meal, vectorial capacity
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Fat Body
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Bloodfeeding
Purdue Medical Entomology
PBM- Post Blood MealNBF- Not Blood Fed
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Hypothesis
Significant metabolomic, transcriptional and metabolic differences exist in the fat body between small and large mosquitoes, before and after blood meal.
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Methods
Metabolic CO2 production
Metabolomic GC/MS, LC/MS, LC/MS/MS analysis
Transcriptomic RNA-Seq approach using Illumina sequencing
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Metabolomics
Questions: Map metabolic pathway up/down regulation
after blood meal
Price et al, unpublished data
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Metabolic
Questions: Do small and large mosquitoes burn energy
at the same rate? Are the substrates being used for energy
the same?
In conjunction with other work: Is there a difference in how far energy stores
go?
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Metabolic
Price et al, unpublished data, Sable Systems
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Transcriptomic
Questions: Determine changes in gene expression
between mosquitoes raised under different nutrient conditions
What patterns of expression are present?
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cathepsin b
Vitellogenic carboxypeptidase Precursor
vitellogenin-B
vitellogenin-C
vitellogenin-A1 precursor
0 5,000,000 10,000,000 15,000,000
Reproduction Transcripts
SPBMLPBM
Read Count
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hypothetical
trypsin
Niemann-Pick Type C-2, putative
serine-type enodpeptidase
Trypsin 3A1 Precursor
Cactus
Relish
Defensin
Cecropin
1 10 100 1,000 10,000 100,000
Immune and Most Changed Transcripts
SNBFLNBF
Read Count (Log10 Scale)
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Conclusions
Used metabolomic, metabolic, and transcriptional descriptive work to identify differences between small and large mosquitoes; pre and post bloodmeal.
Immune regulation is curious Results of small vs large vectorial capacity
experiments have been conflicting Signs of autophagy No apparent metabolic difference
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Hansen Lab/Collaborators
Alexander Ulanov – University of Chicago, Urbana
Wayne Van Voorhies - NMSU molecular Biology
NIAID/NIH