mold and bread science experiment

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    Introduction:

    The sole purpose of this experiment is to see if fungus

    can grow on white crust less bread that has been treated

    with the following substances: apple juice (acid), water(neutral) and liquid hand soap (base).

    The reason I chose these liquids is because these are

    items we us everyday for various purposes. For example we

    drink apple juice because of its vitamins, water is a

    necessity for life and we use soap to sterilize our hands.

    Other than the reasons mentioned earlier, my other

    goal is to see what kind of environment fungi need, or togrow best in.

    In this experiment I used white bread because white

    bread will not interfere with the countless colors of fungi.

    Apple juice has a pH of 3, the water has a pH 0f 7 and

    the liquid hand soap has a pH of 10.

    Hypothesis:

    Using my prior knowledge, I can say that molds an

    only grow on bread that has a neutral substance in it

    because bases are used to bacteria and molds, while acids

    may intervene with the mold spores and may react with it.

    Materials:

    1)

    12 white bread slices [crust less (ingredients: white

    enriched flour, yeast and water)]

    2)

    Concentrate dapple juice [(ACID) ingredients: apple

    juice concentrate, water and sugar]

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    3)

    Liquid hand soap (BASE)

    4)

    Water (NEUTRAL)

    5)

    12 3X4 reclosable bags

    6)3 teaspoons

    7)12 garage sale labels.

    Procedure:

    1)

    Designate each of the teaspoons to the usage of

    basic, neutral and acidic substances.

    2)

    Put a teaspoon of the basic substance on 4 slices of

    bread.

    3)

    Do the same as procedure 2 for the neutral and theacidic substances.

    4)

    Number the reclosable bags 1-12.

    5)Put the 4 slices of basic bread in the bags

    numbered 1-4.

    6)Do the same thing for the neutral bread and

    acidic bread. (Put neutral bread in bags

    numbered 5-8 and put acidic bread in the bags

    numbered 9-12.

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    7)

    Put the 12 garage sale labels on the bags.

    8)

    Write in the labels:

    9)

    Bag number 1: base, warm /light.

    10) Bag number 2: base, warm/dark.

    11) Bag number 3: base, cool/light.

    12)

    Bag number 4: base, cool/dark.

    13) Bag number 5: neutral, warm/light.

    14) Bag number 6: neutral, warm/dark

    15) Bag number 7: neutral, cool/light.

    16)

    Bag number 8: neutral, cool/dark.

    17)

    Bag number 9: acid, warm/light.

    18)

    Bag number 10: acid, warm/dark

    19)

    Bag number 11: acid, cool/light.

    20)

    Bag number 12: acid, cool/dark.

    21)

    Put the bags to its designated environment and

    observe.

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    Data:

    WARM/LIGHT WARM/DARK COOL/LIGHT COOL/DARK

    1) Fungus grewin smallquantities

    around the

    piece of bread,

    except directlyon the basic

    substance

    2) Fungus

    grew, but in

    very little

    quantitiesaround the

    piece of bread.There was an

    absence of

    mold on theplace where a

    basic substance

    was present.

    The color ofthe gray, but in

    some parts itwas black.

    pH

    BASE

    3) Fungus

    completelycovered the

    piece of bread.

    The color of itis gray, but in

    some portions

    its black

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    4) Fungusgrew around

    the edge of

    the piece of

    bread. There

    was anabsence of

    mold directlyon the basic

    substance.

    The mostvisible colors

    of mold are:

    gray, black

    and inextremely

    smallquantities areyellow molds

    5) Nearlycovered by

    fungus or molds

    where theneutral

    substance

    (water) waspresent. The

    color of themold was blackand gray

    NEUTRAL

    6) Fungus grewin small

    amounts

    around the

    piece of bread.In the center of

    the bread is a

    golden kind offungus. The

    most visible

    colors offungus are:

    gray, black,

    yellow, andgolden

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    7) The pieceof bread is

    almost

    covered a

    golden type of

    molds withpatches of

    black and graymolds.

    8) The pieceof bread is

    completely

    covered by amixture of

    golden, black

    and gray

    molds.9) A turquoisetype of fungus

    has completely

    covered thepiece of bread.

    There is also a

    strip of yellow

    fungus

    10) A turquoisetype of fungus

    has completelycovered the

    piece of bread.A white kind of

    fungus is also

    visible.

    ACID

    11) A

    turquoise typeof fungus has

    completely

    covered the

    piece of bread.At the bottom

    of the piece ofbread is some

    golden fungus.

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    12) Aturquoise type

    of fungus has

    completely

    covered the

    piece ofbread. Also,

    there are somepatches a

    golden

    fungus.

    Conclusion:

    After the experiment I now can conclude that fungi

    (molds) can unanimously grow on neutral bread. It cangrow on bread that has been treated with a weak acidic

    substance. Never-the-less molds cannot grow on basic

    bread, so it grows around the edge of the bread where the

    basic substance wasnt present.

    From this experiment I learned that fungus could

    basically grow in any type of environment. I could have

    significantly made this experiment better by carefullycontrolling the temperature and humidity fluctuations.

    Other than using only one type of the basic, neutral and

    acidic substances, I could have used a wide range of them.

    Bibliography:

    I) Websites:

    i)

    Molds.

    http://www.ecy.wa.gov/programs/wq/

    nonprint/(12 Nov 2004)

    ii)

    Mold Guide.

    http://epa.gov/iaq/molds/moldguide.ht

    ml(12 Nov 2004)

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    iii)

    Fungus

    http://www.franklin.com/present.html

    (5 Nov 2004)

    iv)

    Bkgrndhttp://www.geocities.com/~ymjcath/i

    mg/bkgrnd/(5 Nov 2004)

    v)

    Soyhealth

    http://www.planthealth.wise.edu/soyh

    ealth/cause.html(5 Nov 2004)

    vi) Mold01

    http://www.ppdl.purdue.edu/ppdl/wee

    klypics/-504.html(5 Nov 2004)vii) Slime moulds

    http://www.ucmp.berkeley.edu/protist

    a/slimemolds.html(24 Nov 2004)

    viii)20Mold http://www.pnw-

    ag.wsu.edu/smallgrains/Snow%20Mol

    d.html(24 Nov 2004)

    ix)

    Indoor airhttp://www.health.state.mn.us/divs/eh/

    indoorair/mold/(15 Nov 2004)

    x)

    Mold in dry wall

    http://www.emarresources/photos/mol

    dondrywall/(15 Nov 2004)

    II) Books:

    i)

    Money, Nicholas P. Mr.

    Bloomfields Orchard. New York:

    Oxford University Press, 2002.

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    ii)

    Christensen, Clyde M. The Mold and

    Man. Minneapolis: University of

    Minnesota Press, 1975.

    III) Encyclopedia Articles:

    i) Molds Encarta Encyclopedia.

    2004ed.

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