mol.911 molecular biotechnology i - ftp.tugraz.at molecular...5 recent years recombinant antibodies...
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1W I S S E N T E C H N I K L E I D E N S C H A F T
www.tugraz.at
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Engineering of Biosystems
Cell‐EngineeringPathway EngineeringSynthetic Biology
Protein‐Engineering
Engineering of Production Processes
Process DevelopmentProcess MonitoringReaction Engineering
Up‐ and Downstream Processing
BIOTECHNOLOGY
Integrated Technology Development
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http://slideplayer.com/slide/5726826/
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Molecular BiotechnologyIndustrial Biotechnology ‐ Biocatalysis
MolecularBio‐
technology
Bio‐process‐
technology
Fermentation Technology
Enzyme Technology
Processing Technology
Chemical Engineering
Biodiversity ‐ GenomicsBiology
MolecularBiology
BiochemistryEnzymology
StructuralBiology
Catalysis
Kinetics
Org. Synthesis
Chemistryand
Chemical Technology
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Recent Years
Recombinant Antibodies
High Throughput Sequencing
„Omics“
Systems Bio/techno/logy
Synthetic Bio(techno)logy
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Targeted Engineering of Biosystems
Planning in line with engineering practiceExact execution according to construction plans
MolecularBiotechnology
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Engineering of Biosystems
Selection of natural variants
Mutation ‐ random genetic changesrandomly induced mutagenesis
Crosses – genetic RecombinationSexual crossesInduced cell fusionparasexual systems (conjugation, transduction, transformation)
(Targeted) Gene manipulations – Recombinant DNAin vitro Recombination of DNAin vitro site‐specific mutagenesis„designed evolution“
natural diversity
Induced evolution
directed evolution
Synthetic Biology De novo design and construction of complex biosystems/cells
Systems Biotechnology Integrated understanding / modeling / ratio‐based construction of biosystems/cells
Designed biosystems
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8 Engineering of Proteins
EnzymesFunctional Proteins – Biopharmaceuticals
Improvement of the Traits of Enzymes and Proteins byRational Design or Directed Evolution
Stability (T., pH, Solvents …)Activity (vmax , Km …)Substrate specificitySelectivity (Enantioselectivity, position specificity ..)Affinity…. etc.
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Important Cell Functions
Metabolism
Transport
Specialised Compartments‐ Protein clusters‐ Membranes‐ Organelles‐ Cytosceleton
Communication
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GENOME
METABOLOME
PROTEOME
ORGANISMS
TRANSCRIPTOME
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AGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAGAGCTAGCTAG CTAGCTAGCT AGCTAGCTAG CTAGCTAGCT AGCTAGCTAG
1 Gene: 1 page with 2000 letters
Bacterium. 1 book with 2300 pages (4,6 x 106 nucleotides)
Human: 1 library with 717 volumes à 2300 pages (3,3 x 109 nucleotides)
Gene: Amount of Information
MOL.911 Molecular Biotechnology I