modiquest research by jos raats
DESCRIPTION
LTL presents at BioVille: CRO solutions, Six contract research organisations present their servicesTRANSCRIPT
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Expert in immunotechnology, ModiQuest Research is a Contract Research Organisation. Since its establishment in 2004, the company has been the privileged partner of prestigious industrial and academic laboratories for their research, development, diagnostics and therapeutics projects.
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ModiQuest Research at a glance
• LifeScience Park Oss• Company headquarters• Highly Educated & Experienced people• Contract Research Organisation• Communication• Proprietary technologies• Focus on Antibody development
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Antibody generation with a strong focus on low immunogenic and difficult antigens
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Antigen selection
• Peptide design & modeling • Peptide synthesis
• Whole protein
• cDNA immunisation
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Antibody Generation
• Polyclonal Antibodies
• Hybridoma Technology, monoclonal antibodies
• B-cell Selection, monoclonal antibodies
• Phage Display, recombinant monoclonal antibodies
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Monoclonal Antibodies via Hybridoma Technology
Immunization• Various immunization protocols in multiple species
Fusion• Proprietary electrofusion of spleen cells with myeloma cell line• Cells are seeded in semi-solid medium
ClonePix-FL based cell sorting• Viability/ antibody production/ antigen binding• Individual clones are transferred to 96 well plate
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Monoclonal Antibodies via B-cell selection
• Proprietary antigen specific B-cell selection
• Vaccinated, infectious disease, autoimmune • Rabbit, Lama, Mouse, Human
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Monoclonal Antibodies via Phage Display
• Human naïve scFv library
• Human disease scFv libraries - e.g. Rheumatoid Arthritis, Multiple Sclerosis, Scleroderma, SLE, Malaria, Chagas
• Chicken, Mouse, Rabbit scFv libraries - naïve and immune
• Lama libraries - naïve and immune
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Antibody Optimization
• Fusion Protein• Protein Tagging • Chimerisation• Humanisation• Affinity Maturation• Sequencing
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Antibody Production
MQR proprietary expression vectors
Fully human and chimeric antibodies:• Human IgA1, IgM, IgG1, IgG3, and IgG4 (both Kappa & Lambda)• Mouse IgG1, IgG2a, IgG2b, and IgG3• Chicken IgY
Transient production: CHO, HEK-293
Stable cell lines: CHO, HEK-293
Mammalian antibody-expressing cell lines and vectors
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Assay Development
• Animal model: CAIA mouse model• ELISA• Flowcytometry• Bio-assay development
DBA/1 mice treated with a mixture of 8 collagen-specific monoclonal antibodies (2.3 mg total). A control antibody was used at the same concentration. LPS was injected on day 7. Pictures are histological preparations of hind paws 25 days after anti-collagen injection.
SO = Safranin O staining; Blue is bone, red is cartilage. HE = Haematoxylin staining; * = inflammatory infiltrate.
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Antigen Selection
Antibody Generation
Antibody Optimization
Antibody Production
AssayDevelopment
• Peptide Design & Modeling• Peptide synthesis• # different immunization techniques
• Proprietary fusion method• # different species & strains available• # different techniques on different species available
• Polyclonal• Hybridoma monoclonal• B-cell selection, monoclonal• Phage Display, recombinant monoclonal
• Affinity maturation• # different proprietary vectors available for reformatting and/or chimerization• Tagging or fusion• Humanization• Antibody sequencing via RACE
• Many cell lines available• Transient production• Stable cell line
• Gen amplification• Up to grams scale production
• Functionality study• Binding capacity study• Bio-Assay• Animal model
ModiQuest Research Workflow
Antibody Processing
• Labeling• Purification