mixture suppression in blends of sucrose and sinigrin or...

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Mixture suppression in blends of Sucrose and Sinigrin or Goitrin Tove Kjær Beck, Sidsel Jensen, Anette Thybo and Ulla Kidmose Department of Food Science, Aarhus University, Denmark [email protected] Aim To elucidate the interaction between sucrose and two bitter tasting glycosinolates; sinigrin and goitrin, in order to manipu- late with the bitter taste of white cabbage. Conclusion In all four experimental set-ups there is an indication that the intensity of perceived bitterness is reduced by an increase in sucrose concentration. However, this suppression effect of sucrose was only statistically significant in two cases. The ef - ficacy of sucrose seems to depend on the type of bitter ta- sting compound and the test media. Introduction Sucrose is known to have an impact on perceived bitter ta- ste (McBurney & Bartoshuk, 1973). However, it´s influence on the perception of the bitter taste of sinigrin and goitrin, which is present in vegetables such as white cabbage, has not previously been explored. Materials & Methods The samples were studied by quantitative descriptive analysis (QDA), performed by 10 assessors using an unstructured 15 point scale. The sensory and chemical data was analysed by 3-way ANOVA and multivariate data analysis. Results The study indicated in all four experimental set-ups that the in- tensity of perceived bitterness was reduced by increased sucro- se content. However, this suppression effect of sucrose was only statistically significant in two cases, found in both white cabbage juice and in aqueous media for the bitter compound goitrin. The significant results, calculated by 3-way ANOVA (p<0.001), are shown in Figure 1A og 1B. The results showed a good reproducibility and high agreement for sweet and bitter taste among the assessors (Figure 1C). Acknowledgements This study was a part of the project ’MAXVEG’, which is financed by the Danish Strategic Research Council. Reference McBurney, D. H. & Bartoshuk, L. M. (1973). Interactions between stimuli with dif- ferent taste qualities. Phys. & Behav., 10, p.1101-1106. Concentration Concentration [g•I -1 ] [g•I -1 ] - Low Medium High - Medium High Sinigrin 0 0.48 0.96 1.92 Sinigrin 0 0.96 1.92 or or Goitrin 0 0.09 0.18 0.36 Goitrin 0 0.18 0.36 Sucrose 0 9 18 36 Sucrose 0 6 36 Table 1: Full factorial experimental design to test the effect of sucrose on perception of bitterness of sinigrin or goitrin tested in either aqueous solutions or in cabbage juice. Figure 1A - 1B: The result shows a bitter masking effect of sucrose on the perceived bitter taste of the bitter compound goitrin. (A) in aqueous solutions and (B) in cabbage juice. Figure 1C: Tucker1 plot for panel scores for blends of goitrin and sucrose in aquaeous solu- tions. Arrows indicate the directions for the attributes sweet and bitter taste. S0-S3: Ascen- ding sucrose concentrations. BG0-BG3: Ascending goitrin concentrations. B C A Tove - poster.indd 1 29-08-2011 14:51:14

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Mixture suppression in blends of Sucrose and Sinigrin or Goitrin Tove Kjær Beck, Sidsel Jensen, Anette Thybo and Ulla KidmoseDepartment of Food Science, Aarhus University, Denmark

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Aim

To elucidate the interaction between sucrose and two bitter tasting glycosinolates; sinigrin and goitrin, in order to manipu-late with the bitter taste of white cabbage.

Conclusion

In all four experimental set-ups there is an indication that the intensity of perceived bitterness is reduced by an increase in sucrose concentration. However, this suppression effect of sucrose was only statistically significant in two cases. The ef-ficacy of sucrose seems to depend on the type of bitter ta-sting compound and the test media.

Introduction

Sucrose is known to have an impact on perceived bitter ta-ste (McBurney & Bartoshuk, 1973). However, it´s influence on the perception of the bitter taste of sinigrin and goitrin, which is present in vegetables such as white cabbage, has not previously been explored.

Materials & Methods

The samples were studied by quantitative descriptive analysis (QDA), performed by 10 assessors using an unstructured 15 point scale. The sensory and chemical data was analysed by 3-way ANOVA and multivariate data analysis.

Results

The study indicated in all four experimental set-ups that the in-tensity of perceived bitterness was reduced by increased sucro-se content. However, this suppression effect of sucrose was only statistically significant in two cases, found in both white cabbage juice and in aqueous media for the bitter compound goitrin. The significant results, calculated by 3-way ANOVA (p<0.001), are shown in Figure 1A og 1B. The results showed a good reproducibility and high agreement for sweet and bitter taste among the assessors (Figure 1C).

Acknowledgements

This study was a part of the project ’MAXVEG’, which is financed by the Danish Strategic Research Council.

Reference

McBurney, D. H. & Bartoshuk, L. M. (1973). Interactions between stimuli with dif-ferent taste qualities. Phys. & Behav., 10, p.1101-1106.

Concentration Concentration

[g•I-1] [g•I-1]- Low Medium High - Medium High

Sinigrin 0 0.48 0.96 1.92 Sinigrin 0 0.96 1.92

or or

Goitrin 0 0.09 0.18 0.36 Goitrin 0 0.18 0.36

Sucrose 0 9 18 36 Sucrose 0 6 36

Table 1: Full factorial experimental design to test the effect of sucrose on perception of bitterness of sinigrin or goitrin tested in either aqueous solutions or in cabbage juice.

Figure 1A - 1B: The result shows a bitter masking effect of sucrose on the perceived bitter taste of the bitter compound goitrin. (A) in aqueous solutions and (B) in cabbage juice. Figure 1C: Tucker1 plot for panel scores for blends of goitrin and sucrose in aquaeous solu-tions. Arrows indicate the directions for the attributes sweet and bitter taste. S0-S3: Ascen-ding sucrose concentrations. BG0-BG3: Ascending goitrin concentrations.

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Tove - poster.indd 1 29-08-2011 14:51:14