mitosis & meiosis. cell division like begets like (more or less) reproduction occurs at the...
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Mitosis & Meiosis
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Cell Division
• like begets like (more or less)• reproduction occurs at the cellular level
– have to give genetic material to new cell
• asexual• sexual• haploid = one set of DNA (1n)• diploid = 2 sets of DNA (2n)
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Asexual
• production of genetically identical offspring by a single parent
• 1 parent cell (2n) → 2 daughter cells (2n)• DNA is identical• aka: cloning• whole organisms• cells within an organism (growth & repair)
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Sexual
• production of genetically unique offspring– genetic contribution from 2 different parents
• need to reduce the amount of DNA by half– creates gametes (egg & sperm)
• gamete + gamete → new organism• (1n) + (1n) → (2n)
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Prokaryotes: Binary Fission
• single chromosome• circular• small and uncomplicated• stimulus to divide: lots of food
– get too large: own waste = death– need ATP to:
• make new set of DNA• make new cell ‘stuff’
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Figure 8.2B
Prokaryotic chromosomes
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Eukaryotes: Mitosis• larger, more complex cells• large numbers of chromosomes
– chromosomes in pairs: 1 from mom & 1 from dad
• DNA is unwound most of the time: chromatin• duplicate in this form = 4 sets of DNA
• time to divide, need to organize: wind up into chromatids
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Eukaryotes: Mitosis
• cells spend most of their time in Interphase– G1 phase: doing it’s thing but able to start dividing
• G0 phase: doing it’s thing but can’t divide
– S phase: synthesis (duplicate DNA & centrosome)– G2 phase: really getting ready to divide
http://aprenderasbiologia.blogspot.com/2011/01/ciclo-celular.html
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http://www.youtube.com/watch?v=PvDlilBgoSs
http://www.biologyjunction.com/cell_division_notes.htmhttp://usmle-review.org/cell-cycle.php
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Eukaryotes: Mitosis
• Prophase– chromatin condenses into sister chromatids– mitotic spindle forms
• microtubules attach to the centrosomes & centromere
– nuclear membrane breaks down– mitotic spindle starts pushing chromatids toward
middle of ce
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Eukaryotes: Mitosis
• Metaphase– all the centromeres are lined up at equatorial plane
• Anaphase– checkpoint– ATP used to push and pull centromeres toward poles– poles pushed away from each other too– fastest phase
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Eukaryotes: Mitosis
• Telophase– ‘reverse’ of prophase
• chromatids unwind• spindle breaks down• nucleus reforms
• Cytokinesis– contractile ring of actin & myosin forms (hoodie)– plants form a cell plate → cell wall– make more ‘stuff’
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Figure 8.6A
CytokinesisCleavage
furrow Contracting ring ofmicrofilaments
Daughtercells
Cleavagefurrow
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Mitosis: Summary
• start with 1 2n parent• end up with 2 2n daughter cells• DNA in daughter cells is IDENTICAL
– to each other– to parent cell
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Meiosis
• start with 1 2n parent cell• produce 4 1n gametes
– DNA is UNIQUE• each gamete is different than the others• each gamete is different than the parent
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Meiosis
• somatic cells (body cells)– have 2 sets of chromosomes– called homologous pairs
• code for same things, (like eye color) but may have different information (blue or brown)
• 22 pairs of austosomes• 1 pair of sex chromosome (XX in female; XY male)
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Meiosis
• gametes (1n)– have 1 set of chromosomes– meant to pair up with another gamete (1n) to
create a 2n organism
• gamete + gamete → zygote (fertilization) (1n) + (1n) (2n)
gametes created through process of MEIOSIS
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Meiosis
• DNA is copied ONCE• cells divide TWICE
• cross-over: homologous chromosomes swap material = increased genetic diversity
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Mom Dad
http://medakagb.lab.nig.ac.jp/Homo_sapiens/index.html
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Mom Dad
http://medakagb.lab.nig.ac.jp/Homo_sapiens/index.html
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Figure 8.12B
A pair ofhomologouschromosomesin a diploidparent cell
A pair ofduplicatedhomologouschromosomes
Sisterchromatids
1 2 3
INTERPHASE MEIOSIS I MEIOSIS II
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Figure 8.11
Pair of homologouschromosomes
Locus
Centromere
Sisterchromatids
One duplicatedchromosome
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http://click4biology.info/c4b/4/gene4.2.htm
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Meiosis
• Interphase:– chromosomes duplicated– centrosomes duplicated
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Meiosis
• Prophase I:– chromatin coils up– synapsis: 2 sister chromatids come together (tetrad)
• cross over occurs• can take months
– centrosomes go to poles– spindle foms– nuclear envelope breaks down
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Meiosis
• Metaphase I:– tetrads are aligned across from each other at equator
– sister chromatids across from each other– not all centromeres along equator
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Meiosis
• Anaphase I:– sister chromatids pulled away from each other– uses ATP to push/pull chromatids toward poles– uses ATP to push poles away from each other
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Meiosis
• Telophase I and Cytokinesis:– nuclear membrane reforms– chromatids uncoil into chromatin– some species skip this– short interphase (rest)
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Meiosis
• Prophase II:– centosomes duplicate– spindle forms– chromosomes moved toward equator
• Metaphase II:– centromeres aligned on equator
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Meiosis
• Anaphase II:– centromeres of sister chromatids pulled apart– ATP used to push/pull chromatids toward poles– ATP used to push poles away from each other
• Telophase II & Cytokinesis:– nuclei reform– DNA uncoils
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Figure 8.14
Prophase
Metaphase
Duplicatedchromosome(two sisterchromatids)
MITOSIS
Parent cell(before chromosome duplication)
Chromosomeduplication
Chromosomeduplication
Site ofcrossingover
2n 4
Chromosomesalign at themetaphase plate
Tetrads (homologouspairs) align at themetaphase plate
Tetrad formedby synapsis ofhomologouschromosomes
Metaphase I
Prophase I
MEIOSIS I
AnaphaseTelophase
Sister chromatidsseparate during
anaphase
2n 2n
Daughter cells of mitosisNo furtherchromosomalduplication;sisterchromatidsseparate duringanaphase II
n n n n
Daughter cells of meiosis II
Daughtercells of
meiosis I
Haploidn 2
Anaphase ITelophase I
Homologouschromosomesseparate duringanaphase I;sisterchromatidsremain together
MEIOSIS II
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Figure 8.19A_1
Trisomy 21
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Figure 8.20A_s1
Nondisjunction
MEIOSIS I
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Figure 8.20A_s2
Nondisjunction
MEIOSIS I
MEIOSIS II
Normalmeiosis II
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Figure 8.20A_s3
Nondisjunction
MEIOSIS I
MEIOSIS II
Normalmeiosis II
Gametes
Number ofchromosomes
Abnormal gametes
n 1 n 1 n 1 n 1
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Figure 8.20B_s1
Normalmeiosis I
MEIOSIS I
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Figure 8.20B_s2
Normalmeiosis I
MEIOSIS I
MEIOSIS II
Nondisjunction
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Figure 8.20B_s3
Normalmeiosis I
MEIOSIS I
MEIOSIS II
Nondisjunction
Abnormal gametesNormal gametes
n 1 n 1 n n
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