minotour: real-time data analysis for nanopore sequence data...minotour includes a simple to use gui...
TRANSCRIPT
![Page 1: minoTour: real-time data analysis for nanopore sequence data...minoTour includes a simple to use GUI to easy configuration and uploading of sequencing run data in real time into the](https://reader035.vdocuments.us/reader035/viewer/2022070109/60434362bf978604fd757304/html5/thumbnails/1.jpg)
minoTour includesasimpletouseGUItoeasyconfigurationanduploadingof sequencingrundatainrealtimeintotheminoTourmySQLdatabase
1.ABSTRACT 2.GraphicalUserInterface
9.Availability
4.PoreActivityandReadQuality
3.RunSummaries– ReadNumbersandRates 5.AlignmentstoReferenceGenome
7.Anytime,anywhere…
minoTour:real-timedataanalysisfornanoporesequencedata
MartinBlythe,SunirMalla,MichaelStoutandMatthewLooseDeepSEQ,SchoolofLifeSciences,
UniversityofNottingham,Nottingham,NG72UH
8.AskforaDemo@LC2016!
minoTour nowincludes asimpletouseGUItoeasyconfiguration andupload ofsequencingrundataintotheminoTourmySQLdatabase
Othernewfeaturesinclude batchingofBWAalignmentsresultinginsignificantspeedimprovements
minoTour isaplatformforreal-timemonitoring,analysisandinterpretationofsequencedatastreamedfromtheOxford NanoporeminION sequencing device,includingmetricsdescribingsequence qualityandalsoalignmenttoareferencegenomewhereappropriate.minoTourcomprises aWebBrowserinterfaceintoarelationaldatabaseofminIONsequencing datacapturedinreal-timeandincludesaGraphicalUserInterfacetoassistconfigurationofdataupload.minoTour cananalysedatabothpreandpostbasecalling,canremotelynotifyusersofkeyeventsduringsequencing andexploitnovelsequencing featuresmadepossibleforthefirsttimebyminIONdevicesincludingdirectcontrolofthedeviceinreal-time;enabling‘rununtil’experiments.minoTourisopen sourcesoftware,freelyavailableonallmajoroperatingsystemandcommunitydevelopmentisencouraged. AtLondonCalling2016wewilldemonstrateminoTourmonitoringandanalysingminIONsequencing andcontrollingtheminIONsequencer inreal-time.
Awiderangeofsummaryinformation abouteachsequencing runisavailableastherunisprogressing,includingstatisticsonnumbersof andlengthsofreadsalongwithsequencing rates.
minoTour enablesnanoporeactivitiesandreadqualitytobemonitoredinreal-time
UserscansetupTwitteralertsforimportantruneventssuchaswhenaspecific coveragelevelisreached
minoTour cangeneratesummaryplotsformapping readstoareferencegenome usingreadsthathavebeenbasecalledusingmetricore.
Rawpre-basecalled readscanalsobealignedusingdynamictimewarpingofreadeventsdataagainstaputativereferenceeventtrace
6.RemotecontrolofminIONruns
Previoussequencing runscanbestored,managedandarchived,andreadsviewedandexportedasfasta,fastqorSAMfilesfromminoTour’s easytousewebinterface
minoTour canremotelycontroltheminION,startingandstoppingruns,renamingexperiments, andevenmonitoringchannel stateswhilstrunsareprogressing
ResponsivewebtechnologyenablestominoTourwebservicetobeaccessed fromPCs,laptops,tabletsandsmartphones
minoTour canbedownloaded fromhttps://github.com/minoTour/minoTourAhostedminoTourserviceisalsoavailableathttp://minotour.nottingham.ac.uk/[email protected]:Realtimeselectivesequencingusingnanoporetechnology.MatthewLoose,SunirMalla,MichaelStout.BioRXivFeb2016