MINISCAN INSTRUCTIONSDR VIRGINIA GORDONVersion 3, July 2011

General Hints on Running Mini-Scan Tests ..... 1

Acid Number Testing (AN) ............................... 3

f To use the pipettes choose the correct volume for the sample, diluted sample or detector, and place the tip on pipette securely.

f Push the top plunger down and insert pipette below the top of the fluid and let plunger up slowly. Make sure the tip is filled to top. If not, release it and redo. When releasing fluid press down a couple of times.

f Always take a new tip for a new sample and discard the used tips.

f Plug the adaptor into the outlet (110-240) and into the mini-scan unit to charge until the small battery indicator is fully red (charge will be good for 4 hours).

f You will be given a dated sample ID number when you start running samples.

f Record the sample on paper and enter it into the Excel spreadsheet when finished with data retrieval.

f Place the flash drive into the USB port on the mini-scan. To collect data press Done when completing a test and the data will be delivered to the flash drive. To retrieve just insert the flash drive into your computer and download.

f It will appear in Excel format.

f There is space for the tech ID, date and sample identification.

f There is also room with each results for the dilution, sample type, etc., to be added into the Excel format.

GENERAL HINTS ON RUNNING MINI-SCAN TESTS

GENERAL HINTS ON RUNNING MINI-SCAN TESTSONE

PIPETTES

CHARGING THE UNIT

RESULTS RETRIEVAL

f TG kits contain 10 tubes for dilution if necessary (grey caps). f FFA kits contain 4 tubes for dilution (grey caps). f AN kits contains 2 tubes for dilution (grey caps). f All kits contain calibrator tubes (For FFA and AN: 2 yellow-capped C1, 2 green-capped C2, and for TG, 6 clear-capped tubes).

f All kits contain 10 reagent tubes (red TG, Blue FFA, lavender AN). f Use the lower foam insert with tubes as your test tube rack. f Leave bottles and tubes in foam rack for ease of addition.

KITS AND KIT CONTENTS

CALIBRATION

1. Press ‘ACID NUMBER,’ then ‘CALIBRATE,’ then ‘CONTINUE.’

NOTES:• Flash drive should be attached to Mini-Scan if results

need to be saved onto the flash drive.

• If already calibrated, the results will be displayed.

ACID NUMBER TESTING (AN)TWO

ACID NUMBER TESTING (AN)Purpose: To test biodiesel for residual acidity or to test stored biodiesel for further degradation, which raises the AN.

Turn on the mini-scan unit using the top button. Then select the button for the Acid Number Test.

RANGE: 0.2 - 8REPEATABILITY: +/- 0.2CORRELATION TO STANDARD: 0.97

NOTES:

NOTES:

• Always listen for beep before removing tube.

• Draw up solution slowly, making sure there are no air bubbles in pipette tip.

ACID NUMBER TESTING (AN)THREE

2. Insert gray capped tube at prompt for ‘blank tube.’

3. Add 150 uL of Reagent B from brown bottle into yel-low (C1) and green (C2) tube using yellow pipette.

ACID NUMBER TESTING (AN)FOUR

4 . Cap and Shake each tube.

5. Insert green (C1) and yellow (C2) tube into Mini-scan when prompted.

ACID NUMBER TESTING (AN)FIVE

NOTES:• The screen will display the slope, intercept and

correlation and if the calibration is good.

• This calibration is good for about 30 days.

• If calibration fails repeat the calibration.

6. ‘CALIBRATION SUCCEEDED’ will be displayed. Press SAVE.

ACID NUMBER TESTING (AN)SIX

TESTING SAMPLES

1. Press ‘ACID NUMBER TEST,’ then ‘RUN TEST’

2. Insert grey capped tube when prompted for ‘BLANK.’

NOTES:

NOTES:

• Flash drive should be attached to Mini-Scan if results need to be saved onto the flash drive.

• Wait for beep before removing tube.

ACID NUMBER TESTING (AN)SEVEN

NOTES:• Take up sample slowly. If there are bubbles in pipette

tip, redo.

3. Add 100 uL sample into purple capped tube using blue pipette.

4. Put 150 uL Reagent B (brown bottle) into same tube with yellow pipette. Cap and Shake.

ACID NUMBER TESTING (AN)EIGHT

5. Follow prompts to insert sample twice.

5. Press ‘DONE’ if finished or ‘NEXT’ to read another sample.

NOTES:

NOTES:

• Wait for beep before removing tube.

• Unit will quit blinking once results have been saved to flash drive.

• Enter sample ID into the Excel spread-sheet when downloaded.

• Low AN readings are below 0.1 units.

• High AN readings are above 8 units.

For assistance please contact our Support Team:

support@bonanzalabs.com

For further General Information please visit us at:

www.bonanzalabs.com