milad final poweproint presentation for in-house research
TRANSCRIPT
Statement of the ProblemThis study aimed to determine anti-platelet
aggregation activity of Zingiber officinale Rosc. (Ginger) rhizome test solutions using Giemsa microplate assay.
Specifically, this study sought to; 1. determine the number of wells with colorless gels in:
1.1 TS 1 (Hexane-free Extract) 1.2 TS 2 (Ethanol-free Extract) 1.3 TS 3 (Ethyl acetate-free Extract) 1.4 TS 4 (Ginger Pure Extract) 1.5 Positive Control Group (Aspirin) 1.6 Negative Control Group (NSS)
Statement of the Problem Statement of the Problem Cont’d Specific Objectives
Specifically, this study sought to; 2. determine the percentage of anti-platelet aggregation activity of:
2.1 TS 1 (Hexane-free Extract) 2.2 TS 2 (Ethanol-free Extract) 2.3 TS 3 (Ethyl acetate-free Extract) 2.4 TS 4 (Ginger Pure Extract) 2.5 Positive Control Group (Aspirin) 2.6 Negative Control Group (NSS)
Statement of the Problem Statement of the Problem Cont’d Specific Objectives
Specifically, this study sought to; 3. determine the significant difference on the inhibition of platelet aggregation between:
3.1 Positive Control and Test Solution 1 3.2 Positive Control and Test Solution 2 3.3 Positive Control and Test Solution 3 3.4 Positive Control and Test Solution 4
Research Methodology
Figure 1. SWU Pharmacy Laboratory Figure 2. Platelet Concentrate
Figure 3. Giemsa Dye
Research Methodology
Giemsa Microplate Assay Research Methodology
Table 1. Number of Wells with Colorless Gels
Trial TS 1
Hexane-free
Extract
TS 2
Ethanol-
free Extract
TS 3
Ethyl acetate-
free Extract
TS 4
Ginger Pure
Extract
PositiveControl
(Aspirin)
NegativeControl
(NSS)
1 1 0 1 2 2 0
2 2 0 1 2 2 0
3 3 0 2 3 2 0
Results and Discussion
Trial TS 1
Hexane-
free Extract
TS 2
Ethanol-
free Extract
TS 3
Ethyl acetate-
free Extract
TS 4
Ginger Pure
Extract
PositiveControl
(Aspirin)
NegativeControl
(NSS)
1 33.33% 0 33.33% 66.67% 66.67% 0
2 66.67% 0 33.33% 66.67% 66.67% 0
3 100% 0 66.67% 100% 66.67% 0
Percent Average
66.67% 0 44.44% 77.78% 66.67% 0
Table 2. Percentage of Anti-Platelet Aggregation
Activity of the Test Solutions
Positive TS 1
Mean 2 2
Variance 0 1
Observations 3 3
Hypothesized Mean Difference
0
Df 2
t Stat 0
P(T<=t) two-tail 1 Not Significant
t Critical two-tail 4.302655725
Table 3. Significant Difference between Positive
Control and Test Solution 1
Positive TS 2
Mean 2 2
Variance 0 1
Observations 3 3
Hypothesized Mean Difference
0
Df 0
t Stat 65535
P(T<=t) two-tail Undefined Significant
t Critical two-tail Undefined
Table 4. Significant Difference between Positive
Control and Test Solution 2
Positive TS 2
Mean 2 1.33333333
Variance 0 0.33333333
Observations 3 3
Hypothesized Mean Difference
0
Df 2
t Stat 2
P(T<=t) two-tail 0.183503419 Not Significant
t Critical two-tail 4.302655725
Table 5. Significant Difference between Positive
Control and Test Solution 3
Positive TS 2
Mean 2 2.33333333
Variance 0 0.33333333
Observations 3 3
Hypothesized Mean Difference
0
Df 2
t Stat -1
P(T<=t) two-tail 0.422649731 Not Significant
t Critical two-tail 4.302655725
Table 6. Significant Difference between Positive
Control and Test Solution 4
Inferences
After comprehensive analyses and interpretation of
the results, the researchers were able to draw the
following conclusions;
1. Hexane - free and Ethyl acetate - free extracts of
ginger rhizome as well as Ginger pure extract
had been determined to possess anti-platelet
aggregation activity using the Giemsa microplate
assay as established by the absence of violet gels.
Inferences
2. Ginger pure extract has the greatest anti-platelet
aggregation activity among the experimental
group.
3. Out of the three solvents used to extract the
constituents of ginger that have anti-platelet
aggregation activity, hexane yielded the
greatest percent average of anti-platelet
aggregation activity.
Continued
Proposals
With the findings of the study, the researchers
further recommend future researchers of the
following;
1.determine the specific constituent that is responsible for the anti-platelet aggregation activity of Zingiber officinale Rosc.
2.determine the lethal dose of Ginger extract as far as anti-platelet aggregation activity is concerned.
Proposals Continued
3. determine greater statistical difference
between the test solutions used by increasing the number of wells in each trials.
4. determine anti-platelet aggregation activity of ginger using other means aside from the one already used by the researchers.
Proposals Continued
5. conduct more extensive study using more
advanced technology on the mechanism of
anti-platelet aggregation activity of ginger.
