Microscopy

MicroscopyWhat is a microscope?

MicroscopeMicroscope is a tool which can help you see tiny objects and living organisms. It makes them look bigger.

This ability of the microscope is called its magnifying power or magnification.

Light microscopeLight microscope uses diffused light from the sun or artificial light to illuminate the object to be observed.

MicroscopyWhat are the parts of the microscope?

ArmSupports the body tube.

2. BaseSupports and stabilizes themicroscope.

3. Eyepiece / ocular lensMagnifies image producedby objective lens.

4. Body TubeMaintains the proper distance between theobjective and ocularlenses.

Moves stage up and downa large amount for FOCUSING.

5. Coarse adjustment knobMoves stage up and downa tiny amount to SHARPEN the image.

6. Fine adjustment knob7. Light source(lamp or mirror) Provideslight for viewing the slide.Projects light UPWARDS through the diaphragm,the SPECIMEN, and the LENSES.

8. DiaphragmControls the amount of light passing through the slide.

Stage clips - hold the slide in place.Stage- Supports the slidebeing viewed.

9. Stage and stage clips10. Objective lensesFocus and magnifylight coming through theslide.

Rotates to allow use ofdifferent power objectives.

11. Revolving nosepieceSupports the arm and controls the body of the microscope.

12. Inclination jointFollow these instructions when using themicroscope.

To carry the microscope, grasp the ARM with one hand. Hold the BASE with your other hand.

Using the Microscope in 10 Steps10 Steps toUsing the Microscope2. Adjust the DIAPHRAGM while looking through the EYEPIECE.

3. Adjust the LIGHT SOURCE (mirror) until you see the circle of light.

10 Steps to Using the Microscope4. Place the slide on the STAGE.

5. Center the SPECIMEN over the small opening on the STAGE.

6. Secure the slide with the STAGE CLIPS.

10 Steps to Using the Microscope7. While looking at the STAGE from the side, turn the COURSE FOCUS KNOB to lower the BODY TUBE until the LOW POWER OBJECTIVE almost touches the slide.

10 Steps to Using the Microscope8. While looking through the EYEPIECE, carefully move the COURSE FOCUS KNOB until the specimen comes into view.

9. To switch to the HIGH POWER OBJECTIVE LENS, look at the microscope from the side.

10 Steps to Using the Microscope10. Move the NOSEPIECE until the HIGH POWER OBJECTIVE LENS clicks into place. If the lens hits the slide, the slide and/or lens might break. Turn the FINE FOCUS KNOB until the specimen comes into focus.

Specimen

Answer Each Question1. The lens closest to your eye when you use a microscope is the __________.2. When using the microscope, always begin work with the __________ objective first.3. Which knob should you never use while on medium or high power? Explain why.4. The lens closest to the slide is the ___________.5. When using the coarse focus knob, how should you be view the slide? Explain why.6. Carry the microscope with one hand on the ________. and the other on the __________.End of discussion