microscopy supervised by : dr. sherifa prepared by : reem aldossari lama al abdi
DESCRIPTION
Microscopy Supervised by : Dr. Sherifa Prepared by : Reem Aldossari Lama Al abdi. Microscope. Genera!y , It is an instrument used to examine and monitor objects we can’t see with the naked eye. It has many applications. Types of microscopes. - PowerPoint PPT PresentationTRANSCRIPT
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Microscopy
Supervised by : Dr. Sherifa
Prepared by : Reem Aldossari
Lama Al abdi
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Genera!y, It is an instrument used to examine andmonitor objects we can’t see with the naked eye.It has many applications.
Microscope
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There are different classes and types of microscopes dependingon the magnification power, their applications, and how theyare operated:Simple MicroscopesInverted microscope.Light (compound) microscope.Fluorescent Microscope
Types of microscopes
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-Simple Microscopes A magnifying glass, an ordinary double convex lens having a short focal length, is a simple microscope.-A simple microscope has only one lens .
Simple Microscopes
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The compoundmicroscope is made of 3systems:The mounting andmovement System.The magnificationSystem.The iumination System.
Compound Microscope
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The mounting and movement system
The body tube:carries the ocular lenses
The arm:Supports and connects theupper part of the microscope
The coarse focusing knob:for stage movementThe fine focusing knob:for image sharpness
The nose piece:Carries the objective lenses andmove them accordingly above thestageThe stage:Horizontal platform upon whichthe slide of interest rest
The base:Supports the microscope
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The ocular lenses:5X, 10X, and 15X
The objective lenses:Scanning lens: 3.5X or 4X or 5XLow power lens: 10XHigh power lens: 40XOil immersion lens: 100X
THE MAGNIFICATION SYSTEM
How to calculate the magnification power?Magnification power = ocular lens xobjective lens.(e.g.) 10X x 40X = 400X hint: Don’t forget the unit
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THE ILLUMINATION SYSTEM
The illuminator:light source
The iris diaphragm:controls the amount of lightreaching the slide
The condenser:collects and concentrate the light
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The light source and thecondenser are placed ontop of the stage while asthe objective lenses areplaced underneathpointing up.Useful for observing livingcells or organisms at thebottom of a large container.
Inverted Microscope
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Fluorescent MicroscopeThe "fluorescence microscope" refers to any microscope that uses fluorescence to generate an image.
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Histo Chemical stainsGlycogen ( carmine stain )
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Protein ( bromophenol blue )
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Lipids ( Sudan black B)
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Thank you for your attention