1st Summary- Microscopy and Photo microscopy Dr. Robert Ross January 30, 2015

During our last workshop we explored microscopy techniques that would enhance or improve our view into the microscopic world. We were able to differentiate between common examples of light microscopy such as: bright-field, dark-field, phase contrast and Nomarski. Specifically in my group we learned how to set up the bright-field and dark-field microscopes. When referring to the bright-field, this specific illumination technique consists of lighting from beneath the specimen while it’s observed from above. (Meanwhile the dark-field is when both light and electron microscopy that excludes unscattered light beams from the microscope image instead it direct light in conic form towards the specimen and scatters the light). Other than learning about those types of light microscopy, we also explored electron microscopes. Common examples of electron microscopes are the scanning electron microscope (SEM) and transmission electron microscopy (TEM). On the other hand, I was assigned to use the phase contrast technique which consists in shifting the light through a transparent specimen to brightness changes in image. This technique is really useful because it enables us to watch the moving microscopic cells. In order to examine moth wings for florescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation you need to direct it towards a specimen that will absorb a certain range of the electromagnetic spectrum for it to be visible. The florescence microscopy could lead to an advance because it is really useful for biosensor, solar cells, and LED's.

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