microscopes objectives: to name the parts of the microscope and describe their functions
TRANSCRIPT
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Microscopes
Objectives:
•To name the parts of the microscope and describe their functions
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Microscopes
Objectives:
•To name the parts of the microscope and describe their functions
•To describe how to properly use a compound microscope.
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Microscopes
Objectives:
•To name the parts of the microscope and describe their functions
•To describe how to properly use a compound microscope.
•To compare a compound to a light microscope
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focus
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Objective Lenses Scanning = 4X
Low Power = 10X
High power = 40X
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Objective Lenses Scanning = 4X
Low Power = 10X
High power = 40X
Revolving nosepiece
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Label the parts on your microscope picture. Eyepiece/ocular lens
magnification = 10X
Arm-Support
Stage - support
Coarse adjust – general focusFine Focus – high power focus
Base - support
Light source
Diaphragm – adjusts amount of light
Stage clips – holds slide
Objective Lenses Scanning = 4X
Low Power = 10X
High power = 40X
Revolving nosepiece
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Magnification of a Compound Microscope•Because you are looking through multiple lenses the lenses have a “compounding” effect.
•The eyepiece always magnifies 10X
Eyepiece Objective Lens Magnification
Scanning lens (red)
10X
Low Power (yellow)
10X
High Power (blue)
10X
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Magnification of a Compound Microscope•Each of the objective lenses have their own magnification
Eyepiece Objective Lens Magnification
Scanning lens (red)
10X
4X
Low Power (yellow)
10X
10X
High Power (blue)
10X 40X
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Magnification of a Compound Microscope•You then multiply the eyepiece with the objective lens to determine the total magnification
Eyepiece Objective Lens Magnification
Scanning lens (red)
10X
4X
Low Power (yellow)
10X
10X
100XHigh Power (blue)
10X
40X
400X
40X
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Other Laboratory TechniquesOther Laboratory Techniques
Staining– Dyes are added to
slides to bring out detail and stain certain features.
Centrifugation– A device that can spin tubes
up to 20,000X/min. This is used to separate samples (I.e. parts of cells
Cell Culture– Cells of a particular kind
are grown on plates with all their required nutrients.
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Limits of ResolutionLimits of Resolution•As we focus the microscope to higher powers we see more and more detail, until a certain point. Then objects become blurry and detail is lost. Think of blowing up a picture too big.
•
•Resolution is out ability to distinguish two points as separate.
•Electron Microscopes have a much higher limit of resolution.
Click here to zoom
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The Electron MicroscopeThe Electron MicroscopeAllows us to see very high resolution imagesAllows us to see very high resolution images
PREDATORY ANT WITH PEAR PSYLLA
IN ITS MOUTH
SCALES AND SETAE ON LEPIDOPTERA Dust mite
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The Electron MicroscopeThe Electron MicroscopeHow does it work?How does it work?
They use giant electromagnets to sent a stream of electrons over the specimen. This image is then read by a computer.
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General Procedures
Make sure all backpacks are out of the aisles before you get a microscope! Always carry the microscope with one hand on the Arm and one hand on the Base.
Wear your glasses, the microscope will focus to your eyesight!
Keep both eyes open, your brain will learn to ignore the other eye.
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Focusing a Specimen
Always start on low power or scanning.
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Focusing a Specimen
Always start on low power or scanning.
Focus the specimen using the course adjust.
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Focusing a Specimen
Always start on low power or scanning.
Focus the specimen using the course adjust.
Re-center. If high power is needed, turn only after re-centering!
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Focusing a Specimen
Always start on low power or scanning.Focus the specimen using the course adjust.Re-center. If high power is needed, turn
only after re-centering!Now use the FINE FOCUS ONLY for
your final adjustments.
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Making a Wet MountMaking a Wet Mount
1. Gather a very thin slice/piece of whatever your specimen is. All samples should be paper thickness or thinner!
2. Place ONE drop of water directly over the specimen. 3. Place the cover slip at a 45 degree angle with one edge touching the water drop, and let go.
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Making good drawingsMaking good drawings1. Don’t even think of starting your drawing unless you have a
pencil (colored are even better) drawings in pen are unacceptable. This is for two reasons: (a) You can erase pencil! (b) You can shade in areas more easily in pencil
2. In the upper left hand corner of each circle include the specimen name as written on the slide label. In the upper right hand corner, include the magnification (100x or 430x).
3. Label Everything you identify!
100X Cheek cell
nucleus
Cell membrane
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Student handoutsStudent handouts
Microscope notes page
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Microscope Parts
Label with structure and function
Eyepiec
e
Objective
Lens
Magnification
Scanning lens (red)
10X
Low Power (yellow)
10X
High Power (blue)
10X
Magnification of Lenses
Microscopes