Microscope type?SEM TEM CLM

Cheek cell Uses electrons

Uses light

Surface structures

Pollen Internal structures

Mag. Up to 1000X

Mag. 20,000X

Virus

Centrifugation

• Homogenate, supernatant, pellet

Homogenization

Homogenate

Differential centrifugation

Tissuecells

Prokaryotic or Eukaryotic or both

• Nucleoid region• Cell wall• Flagella• DNA• High surface/volume ratio• Nuclear envelope• Single celled• Bacteria• Fermentation• Cytoplasm• Plasma membrane and phospholipid bilayer• Mitochondria• Glycolysis• Oxidative phosphorylation

Active or passive process?

• Diffusion

• Osmosis

• Endocytosis

• Exocytosis

• Pinocytosis

• Phagocytosis

• Facilitated diffusion

• Sodium/ Potassium pump

What is the significance of these proteins?• Integral protein• Peripheral protein• Transmembrane protein• Channel protein• Carrier protein• Glucose transporter• Enzyme• Lysosomal enzyme• Catalase• Microtubules• Microfilaments• Aquaporin• Na/K pump• ATP synthase• Electron transport proteins• Glycolysis Enzymes• Citric Acid Cycle enzymes

What are these types of molecules or substances?

• H2O2

• Cholesterol• Phospholipid• Chromosome• Inorganic cofactor• ATP• ADP• Pyruvate• Glucose• Acetyl CoA• H+• NADH and FADH2

Build a phospholipid bilayer

• Channel protein

• Phospholipids– Hydrophilic heads

– Hydrophobic tails

• Integral protein

• Peripheral protein

• Carrier protein

• Amphipathic protein

• Cholesterol

What process? How do you know?