microscope couplers
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6 5 4 0 B U R R O U G H S .
S TE R L I N G H E I G H TS
M I C H I G A N . U S A . 4 8 3 1 4 2 1 3 3
P H O N E 5 8 6 . 7 3 1 . 6 0 0 0 F A X 5 8 6 . 7 3 1 . 6 4 6 9
E M A I L i n f o @d i a g i n c . c o m
WE B S I TE w w w .d i a g i n c . c o m
For your general digital cameraneeds, request our
SPOT Insight Brochure.
For your substage illuminatorneeds, request our
Transilluminating Bases Catalog.
For your boom stand needs,request our Stereo Microscope
Boom Stands Catalog.
MICROSCOPE COUPLERSF O R V I D E O , D I G I TA L & 35mm C A M E R A S
For your low light digital cameraneeds, request our
SPOT RT Brochure.
inst rument s, inc.
inst rument s, inc.
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COUPLER SERIES SELECTION GUIDE
2
How To Choose:
Select your cameras image capture technology
Select video or high resolution digital
Information You Will Need:
Camera information:
1CCD, 3CCD or 35mm filmCCD sizeAnalog or DigitalCamera lens mount
Microscope information:
Brand, ModelAttachments
Select key features for your application
Go to page numbers indicated for more detail
Appendices1, 2& 3
Pages 34-39
Reference Appendices:
Field of View Diagrams M agnification Equations
Object Size Equations Parfocalization Instructions
1CCD
35mm FILM
ANALOG
(Video)
HR & HRP
DIPS
HRD & HRU
PA1
PAGES14-17 & 20-2
PAGES18-21
PAGES30-33
PAGES8-13
DIGITAL
3CCDIMAGE
CAPTURED BY:ANALOG (Video)
& DIGITALT45 & T60 PAGES
22-29
1.0
xO
NLY
Key Features:
High performance parfocal lens design at an economical price
Positions camera closer to microscope
Available in C-Mount and no-lens 1/2 ENG & Nikon F mounts
Designed for removable phototube microscopes
Key Features:
High performance lens system for high resolution digital cameras
Multi-coated lenses & twist style focus
Available in C and Nikon F mounts
Interchangeable bottom clam ps for nearly all microscope models allows use of one coupler tosupport multiple microscopes
Key Features: Designed specifically to eliminate color shading in three chip camera designs
Multi-coated lenses & twist style focus
Available in C, Sony 1/2 ENG and non-Sony 1/2 ENG lens mounts
Interchangeable bottom clam ps for nearly all microscope models allows use of one coupler tosupport multiple microscopes
Key Features:
Inexpensive and easy to use parfocal designs
Most popular microscope models accommodated
Interchangeable T-mount adapters allow most any 35mm camera to be mounted
Used for applications requiring greater than 1.0x magnification
CDC
Key Features:
Coated lenses & twist style focus Low to moderate price
Available in C and Nikon F mounts
Interchangeable bottom clam ps for nearly all microscope models allows use of one coupler tosupport multiple microscopes
Key Features:
High performance parfocal lens design at an economical price
Designed for consumer digital cameras
Largest selection of microscopes accomodating old and newPAGES
4- 7
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Selected Consumer Digital Camera Couplers for Various Mi croscopes
Benefits of the Consumer Digital Camera System
High Quality
Produces crisp, flat field images with all
microscope objectives including apochromats,
plan fluorites, and achromats.
Economical
Priced up to 50% lower tha n majormanufacturers couplers.
Compact Design
Positions camera close to microscope for great er
stability, convenience a nd a esthetics.
Parfocal
Precise machining provides parfocality
between the microscope and the camera.
Wide Field of View
Use cameras zoom feature to set your
field of view.
Microscope Mounts
The largest selection a vailable.Camera Mounts
28mm x .75 pitch filter thread mount.
37mm x .75 pitch filter thread mount.*
43mm x .75 pitch filter thread mount.**Certain cameras may exhibit vignetting of image.
Note: Older microscopes that use compensating optics
may produce images with lateral chromatic abberat ion
and spherical abberations uncorrected (see Appendix 4).
Nikon Cool Pix Series Camera on a Nikon E200 Microscope
The System . . .
Camera Lens Mount
Choose f rom 28mm , 37mm * and 43mm *filter thread mounts.
Mi croscope Mount
CDC Couplers accommodate nearly any microscope model.
Couplers are ordered a s one catalog number specific for your
microscope (see selection guide on pages 6-7).
Field of View
The cameras zoom system allows you to set y our field of
view close in for detailed image capture or zoom wa y out
for overa ll morphology.
CDCXXNLFor ISO 38mm Phototubes
(Nikon & Leica)
CDCXX23
For 23.2mm Phototubes
CDCXX30For 30mm Phototubes
Selected Consumer Digital Camera Couplers for Various Mi croscopes
CDCXXHC
For Leica HC Tube
CDCXXBX
For Olympus BX/AXCDCXXBB
For Bausch & Lomb Stereozoom 6and Balplan Microzoom
CDCXXAD
For American Optical Diastar,
Microstar IV
CDCXXZNFor Zeiss Axio-2 Se
Computer Optimized Lens System
CD C C oupler systems are computer optimized and expertly
manufactured. All lenses are multicoated optical glass to
ensure crisp, flat field images on your camera.
CD C Series for Consumer D ital Cameras with Filter Thread Mounts
CONSUMER DIGITAL CAMERA COUPLERS /CDC SERIES
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D irect Image P rojection for C -Mount & B ayonet Mount C ameras
1CCD CAMERA COUPLERS /DIP SERIES
Selected Direct Im age Projection Couplers for C-Mount Camera s
Benefits of the Direct Image Projection System
High Quality
Produces crisp, flat field images with all
microscope objectives including apochromats,
plan fluorites, and achromats.
Economical
Price range 13%-250% lower than majormanufacturers couplers.
Low ProfilePositions camera CCD chip at the intermediate
image plane, closer to microscope for greater
stability, convenience, and aesthetics.
Parfocal
Precise machining provides parfocality
between t he microscope and the TV monitor
(see Appendix 4 on Parfocalizing) .
Wide Field of View
0.50x (for 1/2" CC D ) and 0.63x (for 2/3" CC D )
provide wider field of view on monitor.Camera Mounts
C-Mount, Nikon F-Mount, Sony 1/2" ENG Mount
and Non-Sony 1/2" ENG Mount.
Note: Older microscopes that use compensating optics
may produce images with lateral chromatic abbera tion
and spherical abberations uncorrected (see Appendix 4). 0.63x (D63BXC) to attach 2/3" CCD camera to Olympus BX
The System . . .
Camera Lens MountChoose from the large selection of C-mount and
F-mount models.
No Bottom Clamp
The D irect Image Pro jection couplers are ordered as one
part, specific for a range of microscopes. They do not
utilize B ottom Clamps as the HR video coupler series does.
Direct Ima ge Projection Concept
The camera C CD chip is positioned where the microscope
intermediate image is located. This provides a low profile,
stable and more compact system. The system is parfocal
with proper microscope set-up. See Appendix 4 about
Parfocalizing a microscope.
8
D10BXC, D63BXC, D50BXC
For Olympus BX, AX & MX SeriesD10HCC, D63HCC, D50HCC
For Leica DMR, DMRD, DMLS, DMLB
D10NLC, D63NLC, D50NLCFor Nikon & Leica ISO 38mm Ports
Selected Direct Im age Projection Couplers for Nikon F-Mount Cameras
D10ZNC, D63ZNC, D50ZNC
For Zeiss Axiolab, Axioplan-2,Axiophot-2, Axiotech & New Standard
D10HCFFor Leica DMR, DMRD, DMLS, DMLB
D10NEF
For Nikon Eclipse, E400, E600 Series
D10E8FFor Nikon Eclipse, E800, E100 Series
D10BXF
For Olympus BX, AX & MX SeriesD10ZNF
For Zeiss Axiolab, AxioAxiophot-2, Axiotech & New
Direct Image Projection Lens System
For a wider f ield of view, a 0.50x (for 1/2" CC D ) and 0.63x
(for 2/3" CC D ) reduction lenses are ava ilable for all C-mount
versions of the Direct Ima ge Projection series. No -lens
versions are also available which provide a 1.0x magnification.
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1CCD CAMERA COUPLERS /DIP SERIES
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How To Choose:
Select your cameras lens mount
Select your cameras CCD size
Select field of view (to visualize the different field of view sizesachieved with each magnification, see Appendix 2 on pages 36-37)
Go to page numbers indicated (once a magnification is selected,the specific catalog number for your microscope can be found inthe chart on pages 12-13)
Identifythe CCD
format size
1/2"4 .8mm x 6 .4mm
Kodak KAF0402M E
Sony ICX205
1"9 .6mm x 12.8mm
Kodak KAI2000
Kodak KAF3200E
Widest field of view
Smaller, magnifiedfield of view
0.5x
0.63x1.0x (no lens)
Widest field of view
Smaller, magnifiedfield of view
Widest field of view
Smaller, magnifiedfield of view
0.5x
0.63x1.0x (no lens)
0.63x
1.0x (no lens)
2/3"6 .6mm x 8 .8mm
Kodak KAF1402M ESony ICX285
Camera
Lens Mount
C-Mount
Nikon F
Bayonet Mount
Sony 1/2"ENG Bayonet
1/3"3 .6 m m x 4 .8 m m
Coupler Magnification
Non-Sony 1/2"ENG Bayonet
SPOTEnhanced
SP500
SPOT InsightSPOT RT-Mono
SPOTEnhanced
SP600
1.0x (no lens)
1.0x (no lens)
Choose desiredfield of view
(see Appendix 2 forfield of view diagrams)
Identify thmicroscop
pages 12-1order the c
number unddesired mag
tion column.
For widest field of viewsee T45 & T60pages 22-29
For widest field of viewsee T45 & T60pages 22-29
Selection G uide
SPOT RT-ColorSPOT RT-Slider
SPOT EnhancedSP640
For widest field of viewsee HRD & HRU
pages 18-211.0x (no lens)
1"9 .6mm x 12 .8mm
Kodak KAI2000Kodak KAF3200E
2/3"6 .6mm x 8 .8mm
Kodak KAF1402M E
1/2"4 .8mm x 6 .4mm
Kodak KAF0402M E
1.0x (no lens)Widest field of view
SPOT EnhancedSP540
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Monitor vs. E yepiece
APPENDIX 2 / FIELD OF VIEW
Field of View Diagram
In ea ch diagram, the circles represent
the field of view seen through the micro-
scope eyepieces. The rectangles represent
the image that will appear on the TV
monitor (or computer screen).
Ea ch circle corresponds to a certain
field number (FN) eyepiece. Eyepieces
with wider fields have larger field
numbers. The field number is usually
engraved on the e yepiece following the
magnif icatio n, as in WFK 10x/20.
In this example, FN= 20.
Choosing a Magnification
U sually the d esired field of view on
the monitor is that which most closely
corresponds to wha t is seen through the
microscope eyepiece. This is achieved
by selecting the lowest magnification
coupler recommended for a specific chip
size.
However, there are situations that
require a higher magnification coupler.
Specimens that a re blurred on the
monitor are better resolved at a higher
magnification by utilizing more pixels on
the monitor. This is especially useful
when using a 100x objective which is
usually the highest a vailable microscope
magnification. A higher magnification
coupler may also be useful when using a
non-flat field o bjective. This enables the
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1CCD C-Mount 1CCD C-Mount 3CCD B ayonet or C-MountD irect Image Projection Couplers HR /HR P Couplers T45 &T60 Couplers
1CCD C-Mount 1CCD C-Mount 3CCD B ayonet or C-MountD irect Image Projection Couplers HR /HRP Couplers T45 &T60 Couplers
1/3" CCD Cameras
1/2" CCD Cameras
1CCD C-Mount 1CCD C-Mount 3CCD B ayonet MountD irect Image Projection Couplers HR /HR P Couplers HRP100-ENG 23 Coupl
Kodak KAF-0402ME Kodak KAF-1402ME Kodak KAF-3200ESP OT E nha nced -SP 500 & SP 540 SP OT E nha nced -SP 600 & SP 640
Kodak KAI-2000/2001 Sony SonySPOT Insight, SPOT RT ICX205 ICX061, ICX085, IC
2/3" CCD Cameras
Digit al Cameras
user to image only the center (in focus)
portion of the image.
Ultrawide vs. Standard Field of View
What makes an HRU060 ultrawide
field coupler different from an HRD060
standard field coupler? It is the size of
the image the bottom lens captures. The
ultrawide design captures a 26mm
diameter image, whereas a standard
coupler captures a 19mm diameterimage. Capture and correction of this
larger field of view req uires larger and
more exotic (apochromat ic glass) lenses
making the HR U 060 ultrawide coupler
correspondingly more expensive.
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