Sample to Insight

miRNA analysis from single cell

Learn about how you can quantify all (annotated and non-annotated) miRNA from single cell

Sample to Insight

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Discover the miScript Single Cell qPCR Kit

• Generate miRNome expression profiles from a single cell

• Integrated reaction controls for each step of the workflow

• Real-time PCR analysis with the largest portfolio of bench-verified miRNA assays.

Single cell sample input

Universal miRNA cDNA synthesis & preamplification

Tailored data analysis

*MDA = multiple displacement amplification

Single cell genomics by QIAGEN, 2016

Sample to Insight

miScript Single Cell qPCR Kit

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For miRNA expression profiling from a single cell

Primary sample isolation

Single cell isolation

Cell lysis, RT &

miRNA preamp

Sample

• One to 100 cells• 25 pg to 1 ng of purified RNA

• qPCR analysis with miScript miRNA PCR Arrays• Data analysis with GeneGlobe Data Analysis

Center

InsightPCR Data analysis Interpretation

Single cell genomics by QIAGEN, 2016

Sample to Insight

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miScript Single Cell qPCR Kit

PCA reveals a heterogeneous response amongst individual cells to 5-aza-2’-deoxycytidine treatment. HCT 116 colorectal cancer cells were treated ± 5-aza-2’-deoxycytidine (5-aza-2’-dC) demethylation reagent. Bulk cell pellets (Panel A) and individually isolated treated cells (Panel B) were processed using the miScript Single Cell qPCR Kit, and the miFinder miScript miRNA PCR Array was used in real-time PCR. For the bulk sample analysis (Panel A), a scatter plot of 2-ΔCT values demonstrates that differential miRNA expression was observed in response to the 5-aza-2’-dC treatment. PCA analysis of individual cells (Panel B) reveals a differential response amongst the treated cells with three distinct populations. Together, the data suggests the consequential “cellular averages” of bulk analyses mask intrinsic transcriptional variability across individual cells.

Single cell genomics by QIAGEN, 2016

Sample to Insight

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miScript Single Cell qPCR Kit

The miScript Single Cell qPCR Kit enables sensitive detection of low abundance miRNAs. (A) 10-fold dilutions of HeLa S3 purified RNA (1 ng, 100 pg and 10 pg) were processed using the miScript Single Cell qPCR Kit, and the human miFinder miScript miRNA PCR Array was used in real-time PCR. (B) 10-fold dilutions (1x104 copies to 10 copies) of a pool of 24 synthetic miRNAs were processed using the miScript Single Cell qPCR Kit, and miScript Primer Assays were used in real-time PCR. With increasing 10-fold dilutions of RNA or synthetic miRNAs, the CT unit decreased accordingly demonstrating the sensitive detection capacity of the miScript Single Cell qPCR Kit

Single cell genomics by QIAGEN, 2016

Read the white paper Extending miRQC‘s dyamic range: amplifying the view of limiting RNA samples

Sample to Insight

Single cell genomics by QIAGEN

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For in-depth, molecular analysis of single cells

Single cell WGA or WTA

Single cell isolation

Single cell sequencing

• Easily access single cells

• Affordable

• Gentle on cells

QIAscoutQIAseq

FX Single Cell Library Kits

REPLI-g Single Cell Kits

Single cell miRNA analysis

• Create superior-quality NGS libraries directly from single cells

• Provides best-in-class amplification of genomes or transcriptomes from single cells

• Profile miRNA expression using qPCR

miScript Single Cell qPCR Kit

Single cell genomics by QIAGEN, 2016

Visit the single cell resource center