micropatterned surfaces by advanced surface modification€¦ · • antifouling • … 50 mm...
TRANSCRIPT
Micropatterned surfaces by
advanced surface modification
MicroNanoConference
Amsterdam, December 13, 2016
Wout Knoben, PhD
R&D Scientist
T: +31 (0) 85 488 1285
W: www.surfix.nl
• About Surfix
• Micropatterned surfaces
• Surface modification technology
• material-selective
• local
• Summary and conclusions
Outline
confidential 2
confidential
World-changing
micro & nanotechnologies
Control over surface
properties is essential
Make the difference
Improve performance
Create new opportunities
Generate breakthroughs
About Surfix What drives us
Advanced surface
modification technology
3
Performance
- Concentration
- Localization
- Control
confidential
Micropatterned surfaces
4
Surface properties
- Wettability
- Anti(bio)fouling
- Biorecognition
- Cell adhesion
Applications
- Biosensors, analytical and diagnostic devices
- Microfluidics, Lab/Organ-on-a-Chip
- (3D) cell culture
Parallelization
- Miniaturization
- Arraying
- High throughput
Micropatterned surfaces
Self-assembly
(silanes, thiols)
Uniform
modification
confidential 5
Patterning of SAMs
State of the art
Smith et al. Progr Surf Sci 75 (2004) 1-68
Micropatterned surfaces
Local
modification
Triggered assembly
(alkenes, …)
Material-selective
modification
confidential 6
Self-assembly
(silanes, thiols)
Uniform
modification
State of the art
confidential
Biosensor functionalization
Material-selective surface modification
7
SiO2 SiO2 Si3N4 SiO2 SiO2 Si3N4 SiO2 SiO2 Si3N4
• micro ring resonator, Si3N4 waveguide surrounded by SiO2
• waveguide occupies <1% of surface area
• analyte binding on >99% of the sensor is undetected
• improved LoD by material-selective biofunctionalization
confidential 8
F
luore
scence Inte
nsity (
a.u
.)
SiO2 SiO2 Si3N4 SiO2 SiO2 Si3N4 SiO2 SiO2 Si3N4
Material-selective surface modification
Biosensor functionalization
• binding of fluorescently labelled biomolecule
• fluorescence pattern demonstrates concentration of biomolecules on waveguide
confidential 9
Local surface modification
One-step photochemical patterning
• light-triggered surface reaction
• patterning using a photomask
• resistless, single-step process
confidential 10
Local surface modification
Hydrophobic patterns on glass
500 mm500 mm
Local surface modification
confidential
• high chemical contrast • WCA 25/110o
• lateral resolution < 5 mm
• functionalities • wettability
• antifouling
• …
50 mm
Hydrophobic patterns on glass
50 mm
fluorescence microscopy condensation pattern
confidential
Summary and conclusions
12
• surface modification is a key enabling technology for micro- and nanodevices
• Surfix technology enables one-step patterning of surface properties
• multifunctional (micro)patterned surfaces offer exciting opportunities
• hydrophilic/(super)hydrophobic: liquid confinement (arrays, microfluidics)
• biorecognition/antifouling: biosensing
• cell adhesion: (single) cell studies, organ-on-a-chip
For more information
and demos, visit us at
booth 14
13
