micron advanced microscopy course 2011
TRANSCRIPT
MICRON Advanced Microscopy Course 2011
Nature Commincations 2:218Wang et al, March 2011Optical virtual imaging at 50nm lateral resolution with a white-light nanoscope.
“Optical microscopy is one of the most important scientific achievements in the history of mankind.”
http://www.micronoxford.com/
Course organisers and lecturers:Ilan Davis ([email protected]) Ian Dobbie ([email protected])Richard Parton ([email protected])
Additional lecturers:Graeme Ball ([email protected])Mark Howarth ([email protected])Jordan Raff ([email protected])
Assistant Facility manager: Eva WegelImage analysis: Graeme BallCorrelative Light / EM: Rainer Kaufmann
Microscopy
Instrument design (Optics and detectors)
Specimen Preparation (live or fixed, stains)
Probe design (detecting molecules)
Image analysis (post-acquisition)
Milestones in the history of microscopyhttp://www.nature.com/milestones/milelight/pdf/milelight_timeline.pdfhttp://www.nature.com/milestones/milelight/pdf/milelight_all.pdf
13th century when spectacle makers were producing lenses to be worn as glasses.
1st century AD, glass was been invented by the Romans - who then discovered crude lenses.
Zaccharias JanssenGalileo
Ernst AbbeAgust Kohler
1880
Marvin Minsky
Ernst Ruska 1931 EM
Agard and Sedat
Calvin QuateAFM
Martie Chalfie, Roger Chen
Roger Chen
Stefan Hell
JenniferLippincott-Schwartz
Ernst Stelzer
Fritts Zernike1932
Normaski
Nobel Prizes in Microscopy
1903 – Richard Zsigmondy develops the ultramicroscope and is able to study objects below the wavelength of light.The Nobel Prize in Chemistry 1925 »
1932 – Frits Zernike invents the phase-contrast microscope that allows the study of colorless and transparent biological materials.The Nobel Prize in Physics 1953 »
1938 – Ernst Ruska develops the electron microscope. The ability to use electrons in microscopy greatly improves the resolution and greatly expands the borders of exploration.The Nobel Prize in Physics 1986 »
1981 – Gerd Binnig and Heinrich Rohrer invent the scanning tunneling microscope that gives three-dimensional images of objects down to the atomic level.The Nobel Prize in Physics 1986 »
2008 – GFP Shimomura, Tsien, Chalfie
http://nobelprize.org/educational/physics/microscopes/1.html
Day 1: Monday 14 March - Fundamental Principles of MicroscopyStart: 9.30 Coffee and RegistrationIlan 10:00 Introduction to the course1 Richard 10.15-11.15 General introduction to light microscopy2 Ian 11.15-12.30 Principles of microscopy and microscope anatomy12.30-1.30 Lunch and informal discussions3 Ian 1.30-2.30 Contrast enhancement (phase contrast and DIC)4 Ilan 2.30-3.30 Basic Fluorescence Microscopy3.30-4.00 Coffee break5 Richard 4.00-5.00 Basic image analysis5.00-6.00 Informal questions (basic microscopy)***********************************************************************************Day 2: Tuesday 15 March - Imaging Molecules in Cells6 Mark 9.00-10.00 Fluorescent dyes and proteins7 Richard 10.00-11.00 Live cell imaging11.00-11.20 Coffee break8 Jordan 11.20-12.20 Confocal, spinning discs and Multiphotons12.30-1.30 Lunch and informal discussions9 Ilan 1.30-2.30 Advanced widefield microscopy design (and OMX)10 Ian 2.30-3.30 Detectors for microscopy3.30-4.00 Coffee break11 Ian 4.00-5.00 F* techniques: FRET, FLIM, FCS, FRAP, FLIP5.00-6.00 Informal questions***********************************************************************************Day 3: Wednesday 16 March - New and emerging Imaging Approaches12 Howarth 9.00-10.00 Single molecule techniques13 Ilan 10.00-11.00 Breaking the limits: OMX, STED, SPIM, PALM11.00-11.20 Coffee break14 Ian 11.30-12.30 Building bespoke microscopes; image storage12.30-1.30 Lunch and informal discussions15 Graeme 1.30-2.30 Applied Image analysis and MatlabALL 2.30-3.30 Micron and the self-taught practical exercises3.30-4.00 Coffee breakALL 4.00-5.00 Questions and Discussion 6.00 Drinks & nibbles and informal discussions