Microbiology Unknown LabBIO 732

TSI agarTriple Sugar Iron Agar

(a) Red/red

(b) control

(c) Red/yellow

(d) Yellow/yellow

(e) Red/yellow with H2S

A B C D E

Penol Red broths

A B C D E

A (acid with gas)…..B (acid)……C (uninoculated control)……D (no recation)… …E (alkaline reaction)

O-F tests

Control E. coli Pseudomonas Alcaligenes

Fermenter Oxidizer Nonsaccharolytic

Motility Test

Decarboxylation

Positive Negativeuninoculated

Gelatin Hydrolysis

Simmons Citrate

A B C

A: Positive…Enterobacter

B: Negative…E. coli

C: Control-uninoculated

Phenylalanine Deaminase Test

neg pos

Urea agar

pos uninoculated neg

Methyl Red Test

A B

A: Positive (E. coli) B: Negative (Enterobacter aerogenes)

Voges Proskauer Test

A B

A: Negative (E. coli) B: Positive (Enterobacter aerogenes)

Indole TestNegative Positive

- +

Catalase Test

+ -

Positive Negative

GRAM STAIN