microbiology module 7 - bacilli
TRANSCRIPT
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7/30/2019 Microbiology Module 7 - Bacilli
1/87
Microbiology HTL
Bacilli
Gram (+) non-spore forming
Gram (+) spore formingGram (-) bacilli
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Taxonomical Classification:
Gram (+) non-sporeformers
Mycobacterium
Corynebacterium
gravis
mitis
Intermedius
Lactobacillus
Erysipelothrix
Listeria
Gram (+) spore formers:Aerobic - Bacillus
B. anthracis
B. subtilisAnaerobic-Clostridium
C. tetani
C. botulinum
C. perfringens
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Taxonomical Classification: (2)Gram (-) bacilli -
Enterics
Escherichia
Salmonella
Shigella
Klebsiella
Arizona
Providencia
Vibrio
Citrobacter
Edwardsiella
Serratia
Proteus
Morganella
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Small gram (-) bacilli
Bordatella
Hemophilus
Brucella
Pasteurella
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Mycobacterium: (1)M. tuberculosis
(Kochs bacilli)
long, slender,
granular curved orrounded end
AFS (+), Gm (+)
*glycerineLowenstein,
Petroffs, Dorsets ,
Corpers
Types:
Human -man
Bovine - hogs &
cattle
Avian - birds
Pathogenecity: no
toxins produced
Productive Lesions
Exudative Lesion
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AFS property of Mycobacterium:
Due to the presence of mycolic acid.
Mycolic acid gives M. tuberculosis many
characteristics that defy medical treatment.They lend the organisms increased resistance
to chemical damage & dehydration.
Prevent the effective activity of hydrophobicantibiotics
Allows it to grow within macrophages which
makes it resistant to the immune system action
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7/30/2019 Microbiology Module 7 - Bacilli
7/87Microbiology HTL
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7/30/2019 Microbiology Module 7 - Bacilli
8/87Microbiology HTL
Mycobacterium (2)Types of tuberclosis
Primary infection
Reactivation TB
Millary TB
Diagnosis:
microscopic exam
chest x-ray
tuberculin test
culture of sputum
M. leprae ( Lepraebacilli, Hansens bacilli)
long, slender, usually
straight, non-motile,grouped like cigarette
packets
Pathognesis: Leprosy
Acute nodular
Anesthetic or nerve
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7/30/2019 Microbiology Module 7 - Bacilli
10/87Microbiology HTL
Mycobacterium (3) transmission:
prolonged contact
organisms found in
mucous membrane
diagnosis: Mitsuda,
Lepromin test
Stains:Ziehl Neelsen
Baumgarten
Papenheims
M. leprae ( Leprae
bacilli, Hansens
bacilli)
grouped like packetof cigarettes
leprosy:
acute nodular -leproma
anesthetic - nerve
leprosy
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7/30/2019 Microbiology Module 7 - Bacilli
11/87Microbiology HTL
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7/30/2019 Microbiology Module 7 - Bacilli
12/87Microbiology HTL
Corynebacterium: (1)
C.diptheriae: KlebLoeflers bacilli
pleomorphic, globular,
club shaped, bard-shaped,irregular swelling &
staining ---Babe Ernst
bodies
Gram (+), AFS (-)
Culture: BAP, Pais
Tellurite
more in children
Pathogenesis: toxin
is absorbed --
destruction of cells --inflammatory
response -- formation
of pseudomembrane
-- bleeding -- edema
Specimen : nose &
throat swabs
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7/30/2019 Microbiology Module 7 - Bacilli
13/87Microbiology HTL
http://rds.yahoo.com/S=96062857/K=corynebacterium+diphtheria/v=2/SID=w/l=II/R=15/SS=p/OID=3d842a5120b96392/SIG=1iugvco8v/EXP=1122533906/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiphtheria%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-1520085065_q-ZxC94cgHw7ncBI%2Fq9ZIpxAABAA%40%40&h=264&w=410&imgcurl=tooldoc.wncc.edu%2FInfections%2Fanalwart.JPG&imgurl=tooldoc.wncc.edu%2FInfections%2Fanalwart.JPG&size=11.4kB&name=analwart.JPG&rcurl=http%3A%2F%2Ftooldoc.wncc.nevada.edu%2Fmanifest.htm&rurl=http%3A%2F%2Ftooldoc.wncc.nevada.edu%2Fmanifest.htm&p=corynebacterium+diphtheria&type=jpeg&no=15&tt=70&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diphtheria/v=2/SID=w/l=II/R=3/SS=p/OID=82c56fe438996a0e/SIG=1obmcbll4/EXP=1122533906/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiphtheria%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-1520085065_q-ZxC94cgHw7ncBI%2Fq9ZIpxAABAA%40%40&h=254&w=271&imgcurl=www.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Fimage%2Fdiphth2.gif&imgurl=www.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Fimage%2Fdiphth2.gif&size=76.3kB&name=diphth2.gif&rcurl=http%3A%2F%2Fwww.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Furiphyn.htm&rurl=http%3A%2F%2Fwww.kcom.edu%2Ffaculty%2Fchamberlain%2FWebsite%2Flectures%2Flecture%2Furiphyn.htm&p=corynebacterium+diphtheria&type=gif&no=3&tt=70&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diphtheria/v=2/SID=w/l=II/R=1/SS=p/OID=6e2f6213af2c4edc/SIG=1m2dbpsab/EXP=1122533906/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiphtheria%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-1520085065_q-ZxC94cgHw7ncBI%2Fq9ZIpxAABAA%40%40&h=113&w=149&imgcurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fcorynebacterium-diphtheria.jpg&imgurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fcorynebacterium-diphtheria.jpg&size=5.0kB&name=corynebacterium-diphtheria.jpg&rcurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&rurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&p=corynebacterium+diphtheria&type=jpeg&no=1&tt=70&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diptheria/v=2/SID=w/l=II/R=3/SS=p/OID=ce61edfdb1de672a/SIG=1j06tgo07/EXP=1122533859/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiptheria%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=320&w=400&imgcurl=health.yahoo.com%2Fimages%2Fhealth%2Fadam%2Ffullsize%2F9277.jpg&imgurl=health.yahoo.com%2Fimages%2Fhealth%2Fadam%2Ffullsize%2F9277.jpg&size=15.8kB&name=9277.jpg&rcurl=http%3A%2F%2Fhealth.yahoo.com%2Fhealth%2Fency%2Fadam%2F002031%2Fi9277&rurl=http%3A%2F%2Fhealth.yahoo.com%2Fhealth%2Fency%2Fadam%2F002031%2Fi9277&p=corynebacterium+diptheria&type=jpeg&no=3&tt=4&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diptheria/v=2/SID=w/l=II/R=2/SS=p/OID=43a63d792be0bfb8/SIG=1jj0b6g5q/EXP=1122533859/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiptheria%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=112&w=90&imgcurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease%2520%282%29.jpg&imgurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease%2520%282%29.jpg&size=3.8kB&name=diptheria%20disease%20(2).jpg&rcurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcorynebacterium.html&rurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcorynebacterium.html&p=corynebacterium+diptheria&type=jpeg&no=2&tt=4&ei=UTF-8http://rds.yahoo.com/S=96062857/K=corynebacterium+diptheria/v=2/SID=w/l=II/R=1/SS=p/OID=b09311c7a85506e6/SIG=1i12muake/EXP=1122533859/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dcorynebacterium%2Bdiptheria%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=130&w=87&imgcurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease.jpg&imgurl=www.arches.uga.edu%2F%7Ebais%2Fdiptheria%2520disease.jpg&size=3.0kB&name=diptheria%20disease.jpg&rcurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcory2.html&rurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Ebais%2Fcory2.html&p=corynebacterium+diptheria&type=jpeg&no=1&tt=4&ei=UTF-8 -
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Corynebacterium: (2)
Diagnosis:direct
microscopic exam
culturevirulence test -in
vivo & in vitro
DPT vaccine:Diptheria Pertussis
& Tetanus
combination
Methods ofAcquired immunity
fetal transfer
attack orinfection
Artificial
immunizationfrom infection
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Corynebacterium: (3)
gravis mitis intermedius
short in
tellurite
long , granular
in tellurite
long , barred
in tellurite
(-) hemolysis (+) hemolysis (-) hemolysis
large colonies small colonies small colonies
forms pellicle
in broth
grows
diffusely
granular
sediments
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LACTOBACILLUS
-Most species of this non-spore-formingbacterium ferment glucose into lactose,
hence the nameLactobacillus.
-The most common application ofLactobacillus is industrial, i.e. for dairy
production.
-This genus also contains several bacteriathat make up part of the natural flora of the
human vagina..
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Because of their ability to derive lactic acidfrom glucose, these bacteria create an
acidic environment which inhibits growthof many bacterial species which can lead tourogenital infections.
Lactobacillus is generally harmless tohumans, rarely inciting harmful infectionsor diseases.
Treatment of this vancomycin-resistantmicrobe usually consists of high doses ofpenicillin in combination with gentamicin
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http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAhZGJzbkF;_ylu=X3oDMTBjYzZubXM2BHBvcwM4BHNlYwNzcg--/SIG=1i660f029/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=400%26h=500%26imgurl=skywind.wkhc.ac.kr%252F%2525B9%2525CC%2525BB%2525FD%2525B9%2525B0%252FLactobacillus%252520plantarum.jpg%26rurl=http%253A%252F%252Fskywind.wkhc.ac.kr%252F%2525B9%2525CC%2525BB%2525FD%2525B9%2525B0%252Fmicrow.htm%26size=39.3kB%26name=Lactobacillus%20plantarum.jpg%26p=lactobacillus%26type=jpeg%26no=8%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAgJGJzbkF;_ylu=X3oDMTBjcXBoZjEwBHBvcwMzBHNlYwNzcg--/SIG=1eee4aq9t/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=710%26h=710%26imgurl=users.utu.fi%252Fartouwe%252FLGG.JPG%26rurl=http%253A%252F%252Fusers.utu.fi%252Fartouwe%252Ffunctionalfoods.html%26size=44.0kB%26name=LGG.JPG%26p=lactobacillus%26type=jpeg%26no=3%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAh5GJzbkF;_ylu=X3oDMTBkYTNuNGk0BHBvcwMxMARzZWMDc3I-/SIG=1ga459v85/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=135%26h=135%26imgurl=www.laktonova.de%252Flactobacillus_1.jpeg%26rurl=http%253A%252F%252Fwww.laktonova.de%252FProbiotische_Kautabletten_Druc%252Fprobiotische_kautabletten_druc.html%26size=6.1kB%26name=lactobacillus_1.jpeg%26p=lactobacillus%26type=jpeg%26no=10%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAipGJzbkF;_ylu=X3oDMTBkM2llMThuBHBvcwMxMwRzZWMDc3I-/SIG=1gvn5ot4b/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=198%26h=170%26imgurl=biology.clc.uc.edu%252FFankhauser%252FLabs%252FMicrographs%252FLactobacillus_P7121141.jpg%26rurl=http%253A%252F%252Fbiology.clc.uc.edu%252FFankhauser%252FLabs%252FMicrographs%26size=9.0kB%26name=Lactobacillus_P7121141.jpg%26p=lactobacillus%26type=jpeg%26no=13%26tt=9,127%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq7yD21EuUsAf5GJzbkF;_ylu=X3oDMTBjdmNoOTVjBHBvcwMyBHNlYwNzcg--/SIG=1fqu7j9pq/EXP=1148084594/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlactobacillus%2526fr%253DFP-tab-img-t%2526toggle%253D1%2526cop%253D%2526ei%253DUTF-8%26w=288%26h=216%26imgurl=bioweb.usu.edu%252Fmicroscopy%252Flactobacillus%252520brevis.jpg%26rurl=http%253A%252F%252Fbioweb.usu.edu%252Fmicroscopy%252FResearch.htm%26size=18.3kB%26name=lactobacillus%20brevis.jpg%26p=lactobacillus%26type=jpeg%26no=2%26tt=9,127%26ei=UTF-8 -
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ERYSIPELOTHRIX
E. rhusiopathiae, the only species of thisgenus, is better known as a veterinary
pathogen than as a human pathogen. When cultured on blood agar or some other
nutrient medium, Erysipelothrix formsnotably large colonies.
This ubiquitous microbe has been found inmany farm animals such as pigs, horses, andturkeys.
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Occasionaly, though, it can infect a
human host and cause an inflammatoryskin disease, Erysipeloid.
Treatment usually consists of penicillinG, ampicillin, or cephalothin.
Most clinical strains have been found tobe resistant to the super-antibiotic,vancomycin.
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LISTERIA:
Gram-positive rod which is not capable offorming endospores.
Although several species of this bacterium
exist, our discussion will focus only on thetwo species of human pathogenic significance:L. monocytogenes andL. ivanovii.
In particular,L. monocytogenes has beenimplicated in several food poisoningepidemics. This is a normal inhabitant of thegastrointestinal tract and of animal feces
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Infected suffers from vomiting, nausea, and
diarrhea.
Those at high risk include newborns, pregnantwomen and their fetuses, the elderly, and persons
lacking a healthy immune system.
The bacterium usually causes septicema andmeningitis in patients with supressed immune
function.
Causes Listeriosis, an inflammation of the brain.
Antibiotics are recommended for treatment of
infection because most strains ofListeria are
sensitive to ampicillin and gentamicin.
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Microbiology HTL
http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQB1HOJzbkF;_ylu=X3oDMTBkYTNuNGk0BHBvcwMxMARzZWMDc3I-/SIG=1h1u8erfb/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=200%26h=200%26imgurl=www.genomenewsnetwork.org%252Fgnn_images%252Fnews_content%252F07_01%252FListeria_genome_food%252Flisteria.jpg%26rurl=http%253A%252F%252Fwww.genomenewsnetwork.org%252Farticles%252F07_01%252FListeria_genome_food.shtml%26size=14.5kB%26name=listeria.jpg%26p=listeria%26type=jpeg%26no=10%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQB0HOJzbkF;_ylu=X3oDMTBjcDR2NTN2BHBvcwM2BHNlYwNzcg--/SIG=1gk3j96o6/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=250%26h=169%26imgurl=www.techno.msu.ac.th%252FFN%252Fecenter%252Fpathogens%252Fimages%252Fpig-listeria2.jpg%26rurl=http%253A%252F%252Fwww.techno.msu.ac.th%252FFN%252Fecenter%252Fpathogens%252Flisteria__monocytogenes.htm%26size=16.4kB%26name=pig-listeria2.jpg%26p=listeria%26type=jpeg%26no=6%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQBznOJzbkF;_ylu=X3oDMTBjb3ZrYjNkBHBvcwM0BHNlYwNzcg--/SIG=1drh94hlq/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=173%26h=114%26imgurl=www.bact.wisc.edu%252FBact330%252FListeria.TEM2.jpeg%26rurl=http%253A%252F%252Fwww.bact.wisc.edu%252FBact330%26size=21.1kB%26name=Listeria.TEM2.jpeg%26p=listeria%26type=jpeg%26no=4%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQBzXOJzbkF;_ylu=X3oDMTBjcXBoZjEwBHBvcwMzBHNlYwNzcg--/SIG=1fts9k53p/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=553%26h=434%26imgurl=www.foodscience.cornell.edu%252Fwiedmann%252FListeria%252520EM%252520Picture.JPG%26rurl=http%253A%252F%252Fwww.foodscience.cornell.edu%252Fwiedmann%252Fresearch.htm%26size=206.2kB%26name=Listeria%20EM%20Picture.JPG%26p=listeria%26type=jpeg%26no=3%26tt=8,565%26ei=UTF-8http://rds.yahoo.com/_ylt=A9G_Rq9xEW1E5FQBzHOJzbkF;_ylu=X3oDMTBjdmNoOTVjBHBvcwMyBHNlYwNzcg--/SIG=1el5td43d/EXP=1148084977/**http%3a//images.search.yahoo.com/search/images/view%3fback=http%253A%252F%252Fimages.search.yahoo.com%252Fsearch%252Fimages%253Fp%253Dlisteria%2526ei%253DUTF-8%2526fr%253DFP-tab-img-t%2526x%253Dwrt%26w=300%26h=210%26imgurl=www.thebestlinks.com%252Fimages%252F0%252F05%252FListeria.jpg%26rurl=http%253A%252F%252Fwww.thebestlinks.com%252FListeria_monocytogenes.html%26size=28.5kB%26name=Listeria.jpg%26p=listeria%26type=jpeg%26no=2%26tt=8,565%26ei=UTF-8 -
7/30/2019 Microbiology Module 7 - Bacilli
25/87
Microbiology HTL
Bacillus : (1)
B. anthracis (anthrax
bacillus)
Gram (+) , aerobic,
central spore, motile,
non-pathogenic except
anthracis, thermophilic
ends are concave orsquare, forms long
chains, jointed bamboo
cultural char.:medusa head colonies
inverted fir tree in stab
Biochemical char.:
ferment dextrose
indole is not produced
gelatin is liquefied
Note: Find differences
with B. subtilis
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7/30/2019 Microbiology Module 7 - Bacilli
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Microbiology HTL
http://rds.yahoo.com/S=96062857/K=anthrax/v=2/SID=w/l=II/R=1/SS=p/OID=c8c49ee02f02bbac/SIG=1h006ngn3/EXP=1122534183/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Danthrax%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=335&w=480&imgcurl=www.medterms.com%2Fimages%2Fanthrax.jpg&imgurl=www.medterms.com%2Fimages%2Fanthrax.jpg&size=44.6kB&name=anthrax.jpg&rcurl=http%3A%2F%2Fwww.medterms.com%2Fscript%2Fmain%2Fart.asp%3Farticlekey%3D18821&rurl=http%3A%2F%2Fwww.medterms.com%2Fscript%2Fmain%2Fart.asp%3Farticlekey%3D18821&p=anthrax&type=jpeg&no=1&tt=39,354&ei=UTF-8http://rds.yahoo.com/S=96062857/K=anthrax/v=2/SID=w/l=II/R=2/SS=p/OID=b2d68f871be8cfd4/SIG=1hhi4unhr/EXP=1122534183/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Danthrax%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=492&w=445&imgcurl=www.arches.uga.edu%2F%7Emfield%2Fanthrax_weeping_skin.jpg&imgurl=www.arches.uga.edu%2F%7Emfield%2Fanthrax_weeping_skin.jpg&size=53.4kB&name=anthrax_weeping_skin.jpg&rcurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Emfield%2Fbackground.htm&rurl=http%3A%2F%2Fwww.arches.uga.edu%2F%7Emfield%2Fbackground.htm&p=anthrax&type=jpeg&no=2&tt=39,354&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+anthracis/v=2/SID=w/l=II/R=7/SS=p/OID=075c022220b4932a/SIG=1lq2io5g2/EXP=1122534134/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Banthracis%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-4075278827_q-YFR5hiz.a7ShVQrHNVj.GQABAA%40%40&h=399&w=496&imgcurl=www.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FB2.jpg&imgurl=www.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FB2.jpg&size=52.1kB&name=B2.jpg&rcurl=http%3A%2F%2Fwww.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FBacillus.asp&rurl=http%3A%2F%2Fwww.uvp5.univ-paris5.fr%2FMICROBES%2FDiagnostic%2FBacillus%2FBacillus.asp&p=bacillus+anthracis&type=jpeg&no=7&tt=892&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+anthracis/v=2/SID=w/l=II/R=5/SS=p/OID=7a7b60caf1d79878/SIG=1odfd4mpq/EXP=1122534134/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Banthracis%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-4075278827_q-YFR5hiz.a7ShVQrHNVj.GQABAA%40%40&h=298&w=250&imgcurl=www.lbl.gov%2FScience-Articles%2FArchive%2Fassets%2Fimages%2F2004%2FMar-24%2FBacillus_anthracis.jpg&imgurl=www.lbl.gov%2FScience-Articles%2FArchive%2Fassets%2Fimages%2F2004%2FMar-24%2FBacillus_anthracis.jpg&size=119.6kB&name=Bacillus_anthracis.jpg&rcurl=http%3A%2F%2Fwww.lbl.gov%2FScience-Articles%2FArchive%2Fsb-ESD-cataloging-bacteria.html&rurl=http%3A%2F%2Fwww.lbl.gov%2FScience-Articles%2FArchive%2Fsb-ESD-cataloging-bacteria.html&p=bacillus+anthracis&type=jpeg&no=5&tt=892&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacilus+anthracis/v=2/SID=w/l=II/R=3/SS=p/OID=6ea02c1f8252b52a/SIG=1l73q5dfj/EXP=1122534101/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacilus%2Banthracis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=500&w=400&imgcurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacilus_anthracis_3.jpg&imgurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacilus_anthracis_3.jpg&size=34.2kB&name=bacilus_anthracis_3.jpg&rcurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacillus_anthracis_3.htm&rurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fbacillus_anthracis_3.htm&p=bacilus+anthracis&type=jpeg&no=3&tt=5&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacilus+anthracis/v=2/SID=w/l=II/R=2/SS=p/OID=4c9d74bb707c4252/SIG=1hgkhsnvg/EXP=1122534101/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacilus%2Banthracis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=225&w=220&imgcurl=www.euuzlet.hu%2Fillusztracio%2Fbacilusanthracis.jpg&imgurl=www.euuzlet.hu%2Fillusztracio%2Fbacilusanthracis.jpg&size=15.2kB&name=bacilusanthracis.jpg&rcurl=http%3A%2F%2Fwww.euuzlet.hu%2Fszines%2F2001%2Foktober.html&rurl=http%3A%2F%2Fwww.euuzlet.hu%2Fszines%2F2001%2Foktober.html&p=bacilus+anthracis&type=jpeg&no=2&tt=5&ei=UTF-8 -
7/30/2019 Microbiology Module 7 - Bacilli
27/87
Microbiology HTL
Bacillus : (2)Anthrax - lesion like
carbuncle, large ,
black, necrotic
death due tomechanical clogging
of capillaries
Types:Bacteremia -
blood
Cutaneous - skin
Pulmonary - woolsorters disease
Intestinal
Specimen - blood,swab w/ pus,
sputum, feces
Diagnosis:direct microscopic
culture
animal inoculation
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7/30/2019 Microbiology Module 7 - Bacilli
28/87
Microbiology HTL
http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=10/SS=p/OID=c2762cebce330678/SIG=1in233pco/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=116&w=177&imgcurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fbacillus-subtilis.jpg&imgurl=nengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Fbacillus-subtilis.jpg&size=4.0kB&name=bacillus-subtilis.jpg&rcurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&rurl=http%3A%2F%2Fnengjung.kit.ac.kr%2F%7Eckisu%2Ffm%2F05%2Ffm04-5.htm&p=bacillus+subtilis&type=jpeg&no=10&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=7/SS=p/OID=e275f71aa351ac52/SIG=1i11nss4r/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=194&w=295&imgcurl=www.cgr.ki.se%2Fcgr%2Fpersons%2Fraf%2Fimages%2Fbacillus.gif&imgurl=www.cgr.ki.se%2Fcgr%2Fpersons%2Fraf%2Fimages%2Fbacillus.gif&size=55.2kB&name=bacillus.gif&rcurl=http%3A%2F%2Fwww.cgb.ki.se%2Fcgr%2Fpersons%2Fraf%2Fgenomes.html&rurl=http%3A%2F%2Fwww.cgb.ki.se%2Fcgr%2Fpersons%2Fraf%2Fgenomes.html&p=bacillus+subtilis&type=gif&no=7&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=2/SS=p/OID=dc1e81806e621e8e/SIG=1htq3a7f9/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=306&w=274&imgcurl=www2.austincc.edu%2Fmicrobugz%2Fimages%2F03bsubtilis.jpg&imgurl=www2.austincc.edu%2Fmicrobugz%2Fimages%2F03bsubtilis.jpg&size=19.8kB&name=03bsubtilis.jpg&rcurl=http%3A%2F%2Fwww2.austincc.edu%2Fmicrobugz%2F03morphology.html&rurl=http%3A%2F%2Fwww2.austincc.edu%2Fmicrobugz%2F03morphology.html&p=bacillus+subtilis&type=jpeg&no=2&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=1/SS=p/OID=08e2c2ccaa0d19c2/SIG=1klf80tdi/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=253&w=300&imgcurl=www.iccb.state.il.us%2Fpt3%2Fimages%2Fsci%2Fmod11%2Fbacillus_subtilis.jpg&imgurl=www.iccb.state.il.us%2Fpt3%2Fimages%2Fsci%2Fmod11%2Fbacillus_subtilis.jpg&size=12.6kB&name=bacillus_subtilis.jpg&rcurl=http%3A%2F%2Fwww.iccb.state.il.us%2Fpt3%2Fmod%2Fscience%2Fmod_bio111%2Fmod10%2Fp4.html&rurl=http%3A%2F%2Fwww.iccb.state.il.us%2Fpt3%2Fmod%2Fscience%2Fmod_bio111%2Fmod10%2Fp4.html&p=bacillus+subtilis&type=jpeg&no=1&tt=611&ei=UTF-8http://rds.yahoo.com/S=96062857/K=bacillus+subtilis/v=2/SID=w/l=II/R=4/SS=p/OID=4464914072a430b4/SIG=1jhocub4d/EXP=1122534232/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dbacillus%2Bsubtilis%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=142&w=165&imgcurl=www.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbcillus_subtitlis_eu.jpg&imgurl=www.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbcillus_subtitlis_eu.jpg&size=8.2kB&name=bcillus_subtitlis_eu.jpg&rcurl=http%3A%2F%2Fwww.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbacillus_subtilis.htm&rurl=http%3A%2F%2Fwww.arvanitakis.com%2Fgr%2Fc%2Fbac_gal%2Fbacillus_subtilis.htm&p=bacillus+subtilis&type=jpeg&no=4&tt=611&ei=UTF-8 -
7/30/2019 Microbiology Module 7 - Bacilli
29/87
Microbiology HTL
Clostridium : (1)
C. tetani (tetanus ordrumstick bacillus)
motile
medium in length terminal spore
Thioglycolate broth
pathogenesis: fever,pain , Lockjaw,
sardonic smile,
spasm
Diagnosis:
culture
secretion fromlesion - BAP,
Thioglycolate
Prevention:active immunity
with toxoid
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7/30/2019 Microbiology Module 7 - Bacilli
30/87
Microbiology HTL
http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=5/SS=p/OID=32262fab64339fa8/SIG=1ht3mcc41/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=173&w=433&imgcurl=www.bact.wisc.edu%2FBact330%2Fclostridium.jpeg&imgurl=www.bact.wisc.edu%2FBact330%2Fclostridium.jpeg&size=59.2kB&name=clostridium.jpeg&rcurl=http%3A%2F%2Fwww.bact.wisc.edu%2FBact330%2Flecturetetbot&rurl=http%3A%2F%2Fwww.bact.wisc.edu%2FBact330%2Flecturetetbot&p=clostridium+tetani+%26+tetanus&type=jpeg&no=5&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=17/SS=p/OID=bf795e712e414354/SIG=1kvj5rk2h/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=204&w=260&imgcurl=chemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS%2Fctetani.jpg&imgurl=chemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS%2Fctetani.jpg&size=15.8kB&name=ctetani.jpg&rcurl=http%3A%2F%2Fchemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS&rurl=http%3A%2F%2Fchemweb.calpoly.edu%2Fchem%2Fbailey%2F377%2FPagesW02%2FKristenS&p=clostridium+tetani+%26+tetanus&type=jpeg&no=17&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=16/SS=p/OID=40750e874a2f54c6/SIG=1jl38ngpm/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=98&w=120&imgcurl=www.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.jpg&imgurl=www.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.jpg&size=4.2kB&name=tetanus.jpg&rcurl=http%3A%2F%2Fwww.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.htm&rurl=http%3A%2F%2Fwww.aposchinkenplatz.de%2Finformation%2Fimpfen%2Ftetanus.htm&p=clostridium+tetani+%26+tetanus&type=jpeg&no=16&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=14/SS=p/OID=04c50f3453205318/SIG=1h484inba/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=126&w=235&imgcurl=www.pathguy.com%2Flectures%2Ftetanus.jpg&imgurl=www.pathguy.com%2Flectures%2Ftetanus.jpg&size=5.8kB&name=tetanus.jpg&rcurl=http%3A%2F%2Fwww.pathguy.com%2Flectures%2Finfect.htm&rurl=http%3A%2F%2Fwww.pathguy.com%2Flectures%2Finfect.htm&p=clostridium+tetani+%26+tetanus&type=jpeg&no=14&tt=37&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+tetani+%26+tetanus/v=2/SID=w/l=II/R=1/SS=p/OID=d3768a7c68b55948/SIG=1kcb0npe6/EXP=1122534324/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Btetani%2B%2526%2Btetanus%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=102&w=154&imgcurl=www.lek.sk%2Fimg%2Fpictures%2Fgaleria_mikroorganizmy%2Fclostridium_tetani.jpg&imgurl=www.lek.sk%2Fimg%2Fpictures%2Fgaleria_mikroorganizmy%2Fclostridium_tetani.jpg&size=3.5kB&name=clostridium_tetani.jpg&rcurl=http%3A%2F%2Fwww.lek.sk%2Ftemplate.php%3Fidms%3D14%26locate%3D&rurl=http%3A%2F%2Fwww.lek.sk%2Ftemplate.php%3Fidms%3D14%26locate%3D&p=clostridium+tetani+%26+tetanus&type=jpeg&no=1&tt=37&ei=UTF-8 -
7/30/2019 Microbiology Module 7 - Bacilli
31/87
Microbiology HTL
Clostridium: (2)
C. botulinum (Von
Ermengens bacilli)
motile long
subterminal spore
Culture:
Chopped meat
BA medium
Pathogenesis:
food intoxication
food infection
food poisoning
Culture of leftover
food or stool
History of patient
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Microbiology HTL
http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=18/SS=p/OID=434228dc5519fc64/SIG=1lbrfmor0/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=346&w=182&imgcurl=www.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2FImages%2Fclostridium%2520botulinum.GIF&imgurl=www.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2FImages%2Fclostridium%2520botulinum.GIF&size=32.3kB&name=clostridium%20botulinum.GIF&rcurl=http%3A%2F%2Fwww.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2Fstoring.html&rurl=http%3A%2F%2Fwww.cvm.tamu.edu%2Fheb%2Farchive%2F9899%2FProg5%2Fstoring.html&p=clostridium+botulinum&type=gif&no=18&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=19/SS=p/OID=b271ad352f70488e/SIG=1gkquvmj6/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1002&w=750&imgcurl=www.zuova.cz%2Fnrl%2Fpic%2Fnrlpab23_clbo1.jpg&imgurl=www.zuova.cz%2Fnrl%2Fpic%2Fnrlpab23_clbo1.jpg&size=30.2kB&name=nrlpab23_clbo1.jpg&rcurl=http%3A%2F%2Fwww.zuova.cz%2Fnrl%2Fnrlpab23.php&rurl=http%3A%2F%2Fwww.zuova.cz%2Fnrl%2Fnrlpab23.php&p=clostridium+botulinum&type=jpeg&no=19&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=4/SS=p/OID=a8de24202a176b80/SIG=1g1qrch12/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=342&w=341&imgcurl=www.vidyya.com%2F6images%2FbotoxZ.jpg&imgurl=www.vidyya.com%2F6images%2FbotoxZ.jpg&size=102.3kB&name=botoxZ.jpg&rcurl=http%3A%2F%2Fwww.vidyya.com%2Fvol6%2Fv6i348_2.htm&rurl=http%3A%2F%2Fwww.vidyya.com%2Fvol6%2Fv6i348_2.htm&p=clostridium+botulinum&type=jpeg&no=4&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=8/SS=p/OID=5c43b22fec41b18c/SIG=1nuuqaco2/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=220&w=170&imgcurl=biology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fbotulism_160.jpg&imgurl=biology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fbotulism_160.jpg&size=17.3kB&name=botulism_160.jpg&rcurl=http%3A%2F%2Fbiology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fclostridium.htm&rurl=http%3A%2F%2Fbiology.kenyon.edu%2FMicrobial_Biorealm%2Fbacteria%2Fgram-positive%2Fclostridium%2Fclostridium.htm&p=clostridium+botulinum&type=jpeg&no=8&tt=434&ei=UTF-8http://rds.yahoo.com/S=96062857/K=clostridium+botulinum/v=2/SID=w/l=II/R=2/SS=p/OID=9d58162f41cf0228/SIG=1htg6ah2t/EXP=1122534420/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dclostridium%2Bbotulinum%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=100&w=100&imgcurl=www.vcell.de%2Fpics%2Fclostridium_botulinum-.jpg&imgurl=www.vcell.de%2Fpics%2Fclostridium_botulinum-.jpg&size=6.5kB&name=clostridium_botulinum-.jpg&rcurl=http%3A%2F%2Fwww.vcell.de%2Fgesundheitspark%2Fbotulismus.html&rurl=http%3A%2F%2Fwww.vcell.de%2Fgesundheitspark%2Fbotulismus.html&p=clostridium+botulinum&type=jpeg&no=2&tt=434&ei=UTF-8 -
7/30/2019 Microbiology Module 7 - Bacilli
33/87
Microbiology HTL
Clostridium: (3)
C. perfringens (C.welchii,
Frankels or gas
gangrene)
non-motile
short
central spore
Culture media:
same as C. botulinum
pathogenesis
gas gangrene
food infection
Diagnosis:
swab from lesion
& culture
microscopic exam
culture of pus
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Microbiology HTL
Clostridium: (4)
Clostridium difficile:
Causes
pseudomembranecolitis
Relatively resistant to
most antibiotics
Produces cytopathic
toxins and enterotoxin
Diarrhea, to marked
diarrhea to necrosis of
mucosa
Diagnosis
Demonstrate
neutralizeable toxin in
stoolDetecting enterotoxin
by immunoassay
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7/30/2019 Microbiology Module 7 - Bacilli
35/87
Microbiology HTL
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7/30/2019 Microbiology Module 7 - Bacilli
36/87
Microbiology HTL
Actinomyces israelii
Causes actinomycosis
Vary considerably in length
Maybe branched or un branched
Grows slowly, on agar some strains produce
colonies similar to molar teeth
Sometimes confused with corynebacterium
Sensitive to penicillin & erythromycin
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7/30/2019 Microbiology Module 7 - Bacilli
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Microbiology HTL
Propionibacterium acne:
Normal flora of the skin but can cause
disease when they infect shunt and
appliancesOn gram stain, highly pleomorphic
Participates in the genesis of acne
May contaminate blood and CSF once itpenetrates the skin
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7/30/2019 Microbiology Module 7 - Bacilli
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Microbiology HTL
Eubacterium, Bifidobacterium &
Arachnia:These 3 genera are made up of anaerobic,
pleomorphic, gram positive rods
There are several speciesAre found in mixed infections associated
with oropharyngeal or bowel flora
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Microbiology HTL
Gram - Bacilli
Enteric Bacteria
(Enterobacter family) found
in intestines of man &animals, parasitic &
saprophytic, mostly
pathogenic
causes intestinal diseases &
septicemic infections
produce Hydrogen sulfide
Media:
Selenite broth
Tetrathionate broth
Peptone
Tryptone
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Microbiology HTL
http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=20/SS=p/OID=d87696179923d552/SIG=1hc1kh146/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=619&w=800&imgcurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI002.JPG&imgurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI002.JPG&size=126.8kB&name=I002.JPG&rcurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&rurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&p=enterobacter&type=jpeg&no=20&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=19/SS=p/OID=e6cadd014c61ade6/SIG=1hchprmqd/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=607&w=800&imgcurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI001.JPG&imgurl=conganat.uninet.edu%2Fautores%2Ftrabajos%2FT068%2FI001.JPG&size=108.3kB&name=I001.JPG&rcurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&rurl=http%3A%2F%2Fconganat.uninet.edu%2Fautores%2Ftrabajos%2FT068&p=enterobacter&type=jpeg&no=19&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=1/SS=p/OID=46c223c312bb37e4/SIG=1g3on3t93/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=304&w=340&imgcurl=anka.livstek.lth.se%3A2080%2Fe-aer61.jpg&imgurl=anka.livstek.lth.se%3A2080%2Fe-aer61.jpg&size=36.6kB&name=e-aer61.jpg&rcurl=http%3A%2F%2Fanka.livstek.lth.se%3A2080%2FE_aerogenes.htm&rurl=http%3A%2F%2Fanka.livstek.lth.se%3A2080%2FE_aerogenes.htm&p=enterobacter&type=jpeg&no=1&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=2/SS=p/OID=99ebe730be7f5276/SIG=1ibtb1srl/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=230&w=350&imgcurl=www.visualsunlimited.com%2Fimages%2Fwatermarked%2F673%2F67319.jpg&imgurl=www.visualsunlimited.com%2Fimages%2Fwatermarked%2F673%2F67319.jpg&size=14.5kB&name=67319.jpg&rcurl=http%3A%2F%2Fwww.visualsunlimited.com%2Fbrowse%2Fvu673%2Fvu67319.html&rurl=http%3A%2F%2Fwww.visualsunlimited.com%2Fbrowse%2Fvu673%2Fvu67319.html&p=enterobacter&type=jpeg&no=2&tt=252&ei=UTF-8http://rds.yahoo.com/S=96062857/K=enterobacter/v=2/SID=w/l=II/R=6/SS=p/OID=46b0c92d91f2cf00/SIG=1lfk1vc3v/EXP=1122534790/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Denterobacter%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=772&w=580&imgcurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347enterobacter1.jpg&imgurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347enterobacter1.jpg&size=120.6kB&name=347enterobacter1.jpg&rcurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&rurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&p=enterobacter&type=jpeg&no=6&tt=252&ei=UTF-8 -
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Microbiology HTL
Gram (-) bacilli
Plating media:
Desoxycholate,
CitrateSSA, EMB, BAP
McConkey,
Brilliant green
Indicators:
Neutral red:
Red/colorlessPhenol red:
yellow/red
Andrades: pink/colorless
-
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Microbiology HTL
Escherichia
Escherichia coli
found in the normal
flora of GIT, outsidethe GIT it causes
cystitis, peritonitis
index for food & water
pollution IMViC - ++--
A.aerogenes - --++
http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=3/SS=p/OID=4244d8c63d6c8a70/SIG=1kkcle5ud/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=200&w=250&imgcurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fescherichia_coli_1s.jpg&imgurl=www.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fobr%2Fescherichia_coli_1s.jpg&size=7.8kB&name=escherichia_coli_1s.jpg&rcurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fescherichia_coli.htm&rurl=http%3A%2F%2Fwww.biotox.cz%2Ftoxikon%2Fbakterie%2Fbakterie%2Fescherichia_coli.htm&p=escherichia+coli&type=jpeg&no=3&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=8/SS=p/OID=711d48c1661fee08/SIG=1i1ef2tmt/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=182&w=150&imgcurl=tagc.univ-mrs.fr%2FBioInteractif%2Fpub%2F_e-colism.jpg&imgurl=tagc.univ-mrs.fr%2FBioInteractif%2Fpub%2F_e-colism.jpg&size=25.1kB&name=_e-colism.jpg&rcurl=http%3A%2F%2Ftagc.univ-mrs.fr%2FBioInteractif%2Fpub%2Fpub_pole.html&rurl=http%3A%2F%2Ftagc.univ-mrs.fr%2FBioInteractif%2Fpub%2Fpub_pole.html&p=escherichia+coli&type=jpeg&no=8&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=4/SS=p/OID=183ce63581708200/SIG=1iflcgsu7/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=398&w=400&imgcurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&imgurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&size=59.4kB&name=Ecoli.jpg&rcurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&rurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&p=escherichia+coli&type=jpeg&no=4&tt=4,399&ei=UTF-8 -
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Qualitative analysis of
water:
Organolytic - physical
Presumptive - lactose
broth for gas
Confirmatory - EMB
Completed - RDS,
TSI, KIA
http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=5/SS=p/OID=9ce15ab2acad45a4/SIG=1gjirtmom/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=256&w=384&imgcurl=www.historique.net%2Fmicrobes%2Fimages%2Fgas.jpg&imgurl=www.historique.net%2Fmicrobes%2Fimages%2Fgas.jpg&size=24.8kB&name=gas.jpg&rcurl=http%3A%2F%2Fwww.historique.net%2Fmicrobes%2Fgas.html&rurl=http%3A%2F%2Fwww.historique.net%2Fmicrobes%2Fgas.html&p=escherichia+coli&type=jpeg&no=5&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=4/SS=p/OID=183ce63581708200/SIG=1iflcgsu7/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=398&w=400&imgcurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&imgurl=res2.agr.ca%2FLethbridge%2Femia%2Fimages%2FSEMproj%2FEcoli.jpg&size=59.4kB&name=Ecoli.jpg&rcurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&rurl=http%3A%2F%2Fres2.agr.ca%2FLethbridge%2Femia%2FSEMproj%2Fecoli_e.htm&p=escherichia+coli&type=jpeg&no=4&tt=4,399&ei=UTF-8http://rds.yahoo.com/S=96062857/K=escherichia+coli/v=2/SID=w/l=II/R=1/SS=p/OID=f3e2f826540590fe/SIG=1g989a6a8/EXP=1122534878/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Descherichia%2Bcoli%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=393&w=500&imgcurl=www.siue.edu%2F%7Ecbwilso%2FEcoli.jpg&imgurl=www.siue.edu%2F%7Ecbwilso%2FEcoli.jpg&size=36.3kB&name=Ecoli.jpg&rcurl=http%3A%2F%2Fwww.siue.edu%2F%7Ecbwilso%2F250graphics00.htm&rurl=http%3A%2F%2Fwww.siue.edu%2F%7Ecbwilso%2F250graphics00.htm&p=escherichia+coli&type=jpeg&no=1&tt=4,399&ei=UTF-8 -
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Microbiology HTL
Salmonella Salmonella typhosa
circular, small,
smooth surface w/
even edge colonies Indole (-), Citrate
(+), no lactose
fermentation,
ferments glucose,
maltose
http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=1/SS=p/OID=b1cc0d89803d81ca/SIG=1mocgfhsp/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=490&w=591&imgcurl=www.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella%2520typhi%252010cm.jpg&imgurl=www.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella%2520typhi%252010cm.jpg&size=100.9kB&name=salmonella%20typhi%2010cm.jpg&rcurl=http%3A%2F%2Fwww.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella_typhi_zoom_page.htm&rurl=http%3A%2F%2Fwww.gastrohygiene.li%2Fmikroorganismen%2Fsalmonella_typhi_zoom_page.htm&p=salmonella+typhi&type=jpeg&no=1&tt=172&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=5/SS=p/OID=332c044960cba4b8/SIG=1kt4inpg7/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=222&w=160&imgcurl=fr.wikipedia.org%2Fupload%2F8%2F8c%2FSalmonella_enterica.jpg&imgurl=fr.wikipedia.org%2Fupload%2F8%2F8c%2FSalmonella_enterica.jpg&size=48.9kB&name=Salmonella_enterica.jpg&rcurl=http%3A%2F%2Ffr.wikipedia.org%2Fwiki%2FFi%25C3%25A8vre_typho%25C3%25AFde&rurl=http%3A%2F%2Ffr.wikipedia.org%2Fwiki%2FFi%25C3%25A8vre_typho%25C3%25AFde&p=salmonella+typhi&type=jpeg&no=5&tt=172&ei=UTF-8 -
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Microbiology HTL
Antigenic Structure:H-flagellar
O-somatic
Vi-capsular
Widals Test:
^titer O - active
infection
^titer H - postvaccination
^Vi -carrier
http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=9/SS=p/OID=f6c7066c85bcf18e/SIG=1nv19n472/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=173&w=200&imgcurl=www.ecplanet.com%2Fpic%2F2003%2F01%2F1042201189%2Fsalmonella_typhimurium.jpg&imgurl=www.ecplanet.com%2Fpic%2F2003%2F01%2F1042201189%2Fsalmonella_typhimurium.jpg&size=6.2kB&name=salmonella_typhimurium.jpg&rcurl=http%3A%2F%2Fwww3.ecplanet.com%2Fcanale%2Fsalute-7%2Fpatologie-68%2F0%2F0%2F4900%2Fit%2Fchiasso_line.xml&rurl=http%3A%2F%2Fwww3.ecplanet.com%2Fcanale%2Fsalute-7%2Fpatologie-68%2F0%2F0%2F4900%2Fit%2Fchiasso_line.xml&p=salmonella+typhi&type=jpeg&no=9&tt=172&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=4/SS=p/OID=de1a3a763177e736/SIG=1i4oks9bt/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=193&w=197&imgcurl=www.dmacgroup.com%2FSalmonella%2520Typhi.jpg&imgurl=www.dmacgroup.com%2FSalmonella%2520Typhi.jpg&size=27.1kB&name=Salmonella%20Typhi.jpg&rcurl=http%3A%2F%2Fwww.dmacgroup.com%2Ftyphoid.htm&rurl=http%3A%2F%2Fwww.dmacgroup.com%2Ftyphoid.htm&p=salmonella+typhi&type=jpeg&no=4&tt=172&ei=UTF-8 -
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Microbiology HTL
SalmonellaResistance:
killed at 60
degrees for 15 - 20
minutes
can survive whole
winter, 7 days in
well water
Typhoid & entericfever- S.typhosa, S.
para A, S. para B
http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=11/SS=p/OID=20e9a0b452df5122/SIG=1l8htksf3/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=302&w=302&imgcurl=www.spiceisle.com%2Fhomepages%2Fzross%2FMAC%2520Sal%2520typhi.jpg&imgurl=www.spiceisle.com%2Fhomepages%2Fzross%2FMAC%2520Sal%2520typhi.jpg&size=15.4kB&name=MAC%20Sal%20typhi.jpg&rcurl=http%3A%2F%2Fwww.spiceisle.com%2Fhomepages%2Fzross%2FEnteric%2520Demo.htm&rurl=http%3A%2F%2Fwww.spiceisle.com%2Fhomepages%2Fzross%2FEnteric%2520Demo.htm&p=salmonella+typhi&type=jpeg&no=11&tt=172&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+typhi/v=2/SID=w/l=II/R=3/SS=p/OID=1f69b132783a9bec/SIG=1makhs926/EXP=1122535007/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Btyphi%26sp%3D1%26ei%3DUTF-8%26fl%3D0%26fr%3DFP-tab-img-t-283%26SpellState%3Dn-2481628037_q-X64O6BvAYaSHj.HChHAX7AABAA%40%40&h=400&w=500&imgcurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FSalmonella%2520typhi.jpg&imgurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FSalmonella%2520typhi.jpg&size=21.4kB&name=Salmonella%20typhi.jpg&rcurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&rurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&p=salmonella+typhi&type=jpeg&no=3&tt=172&ei=UTF-8 -
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Salmonella
Paratyphoid fever -
S. paratyphosa
Gastroenteritis &food poisoning - S.
derby, S. enteritidis
Bacteremia,
septicemia- S.
Cholrasuis
Characteristics in food
poisoning: no taste or
smell, grow rapidly in
meat, toxins resistantover 100 degrees, not
destroyed with ordinary
cooking
Sources: water, milk &
dairy products, frozen
eggs, meat
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Microbiology HTL
Salmonella Shigella
Motility non-motile motile
Hydrogen
sulfide
positive negative
Indol negative variable
Citrate positive negativeMannitol non fermenter fermenter
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Microbiology HTL
http://rds.yahoo.com/S=96062857/K=salmonella+shigella+agar/v=2/SID=w/l=II/R=10/SS=p/OID=8c3633bbf60ddea2/SIG=1ilvnuq7g/EXP=1122535130/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Bshigella%2Bagar%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=446&w=437&imgcurl=www.euronet.nl%2Fusers%2Fwarnar%2Fbacterialplates.jpg&imgurl=www.euronet.nl%2Fusers%2Fwarnar%2Fbacterialplates.jpg&size=25.0kB&name=bacterialplates.jpg&rcurl=http%3A%2F%2Fhome.euronet.nl%2Fusers%2Fwarnar%2Fbiotechnologie2.htm&rurl=http%3A%2F%2Fhome.euronet.nl%2Fusers%2Fwarnar%2Fbiotechnologie2.htm&p=salmonella+shigella+agar&type=jpeg&no=10&tt=12&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+shigella+agar/v=2/SID=w/l=II/R=2/SS=p/OID=e4ce39edb0155db0/SIG=1lvqav0hb/EXP=1122535130/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Bshigella%2Bagar%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=84&w=125&imgcurl=www.laboratoriosmicrokit.com%2Fimages%2F1de3%2F07-SS%2520Agar-E.coli%2520y%2520Salmonellap.jpg&imgurl=www.laboratoriosmicrokit.com%2Fimages%2F1de3%2F07-SS%2520Agar-E.coli%2520y%2520Salmonellap.jpg&size=12.1kB&name=07-SS%20Agar-E.coli%20y%20Salmonellap.jpg&rcurl=http%3A%2F%2Fwww.laboratoriosmicrokit.com%2Fdocs%2FTitle%2BGF-08b.htm&rurl=http%3A%2F%2Fwww.laboratoriosmicrokit.com%2Fdocs%2FTitle%2BGF-08b.htm&p=salmonella+shigella+agar&type=jpeg&no=2&tt=12&ei=UTF-8http://rds.yahoo.com/S=96062857/K=salmonella+shigella+agar/v=2/SID=w/l=II/R=6/SS=p/OID=9057b899882709a6/SIG=1nfgq5eoj/EXP=1122535130/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dsalmonella%2Bshigella%2Bagar%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=459&w=648&imgcurl=www.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fapplied%2520images%2Fsalmshigella.png&imgurl=www.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fapplied%2520images%2Fsalmshigella.png&size=1.1MB&name=salmshigella.png&rcurl=http%3A%2F%2Fwww.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fschedulelinks%2Fsalmonellashigella.htm&rurl=http%3A%2F%2Fwww.rci.rutgers.edu%2F%7Emicrolab%2Fapplied%2520micro%2Fschedulelinks%2Fsalmonellashigella.htm&p=salmonella+shigella+agar&type=png&no=6&tt=12&ei=UTF-8 -
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Shigella
Shigella dysenteriae:
small, convex,
circular, colorless,glistening colonies
non-lactose
fermenter, body
temp. , aerobic
ferment glucose,
mannitol, citrate (-)
http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=12/SS=p/OID=685b6ac33decd958/SIG=1juo5gco4/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=400&w=500&imgcurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FShigella%2520sonnei.jpg&imgurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FShigella%2520sonnei.jpg&size=16.0kB&name=Shigella%20sonnei.jpg&rcurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&rurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&p=shigella&type=jpeg&no=12&tt=643&ei=UTF-8 -
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Microbiology HTL
Mannitol fermentation
(-) Indole
(-) S. dysenteriae
(+) S. ambigua
(+) Lactose
(-) Indole
(-) S. flexner
(+) S. boydii
(+) Indole
(-) S. sonnei
http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=7/SS=p/OID=39457cd217194c2a/SIG=1j2r3u43h/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=320&w=400&imgcurl=medicalimages.allrefer.com%2Flarge%2Fdigestive-system.jpg&imgurl=medicalimages.allrefer.com%2Flarge%2Fdigestive-system.jpg&size=17.1kB&name=digestive-system.jpg&rcurl=http%3A%2F%2Fhealth.allrefer.com%2Fhealth%2Fshigella-enteritis-digestive-system.html&rurl=http%3A%2F%2Fhealth.allrefer.com%2Fhealth%2Fshigella-enteritis-digestive-system.html&p=shigella&type=jpeg&no=7&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=2/SS=p/OID=9c423650b0234ef0/SIG=1j80uum1k/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=120&w=160&imgcurl=toronto.cbc.ca%2Fgfx%2FToronto%2Fphotos%2Fshigella_052002.jpg&imgurl=toronto.cbc.ca%2Fgfx%2FToronto%2Fphotos%2Fshigella_052002.jpg&size=9.1kB&name=shigella_052002.jpg&rcurl=http%3A%2F%2Ftoronto.cbc.ca%2Fregional%2Fservlet%2FView%3Ffilename%3Dshigella_310502&rurl=http%3A%2F%2Ftoronto.cbc.ca%2Fregional%2Fservlet%2FView%3Ffilename%3Dshigella_310502&p=shigella&type=jpeg&no=2&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=3/SS=p/OID=a94a660e134e4926/SIG=1h76g1k5o/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1258&w=1804&imgcurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&imgurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&size=961.7kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&rurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&p=shigella&type=jpeg&no=3&tt=643&ei=UTF-8 -
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Microbiology HTL
Diagnosis :
Stool exam, rectal swab --
culture in McConkey, SSAor EMB
Pathogenesis: bacillary
dysentery- abdominal
pain, cramps, diarrhea,
fever
http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=3/SS=p/OID=a94a660e134e4926/SIG=1h76g1k5o/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1258&w=1804&imgcurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&imgurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&size=961.7kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&rurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&p=shigella&type=jpeg&no=3&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=3/SS=p/OID=a94a660e134e4926/SIG=1h76g1k5o/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=1258&w=1804&imgcurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&imgurl=www-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro%2Fshigella.jpg&size=961.7kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&rurl=http%3A%2F%2Fwww-instruct.nmu.edu%2Fcls%2Flriipi%2Fmicro&p=shigella&type=jpeg&no=3&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=20/SS=p/OID=4befa7f204ac847c/SIG=1gi95af47/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=125&w=100&imgcurl=www.lcusd.com%2Flchs%2Fmewoldsen%2Fshigella.jpg&imgurl=www.lcusd.com%2Flchs%2Fmewoldsen%2Fshigella.jpg&size=3.0kB&name=shigella.jpg&rcurl=http%3A%2F%2Fwww.lcusd.com%2Flchs%2Fmewoldsen%2FFarag.html&rurl=http%3A%2F%2Fwww.lcusd.com%2Flchs%2Fmewoldsen%2FFarag.html&p=shigella&type=jpeg&no=20&tt=643&ei=UTF-8http://rds.yahoo.com/S=96062857/K=shigella/v=2/SID=w/l=II/R=4/SS=p/OID=3343a29f324f2818/SIG=1l1048cm5/EXP=1122535196/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3D%2Bshigella%2B%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=772&w=580&imgcurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347shigella1.jpg&imgurl=www.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2Fgraphics%2F347shigella1.jpg&size=110.9kB&name=347shigella1.jpg&rcurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&rurl=http%3A%2F%2Fwww.umanitoba.ca%2Fscience%2Fmicrobiology%2Fstaff%2Fcameron%2F60_347.htm&p=shigella&type=jpeg&no=4&tt=643&ei=UTF-8 -
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Microbiology HTL
ArizonaArizona hinshawii
Gram (-) , motile,
similar to Salmonella
Slow lactosefermenter, sucrose &
Indole (-)
IMViC - -+-+Pathogenesis: causes
gastroenteritis or
sepsis
SPACE
FOR
RENT!!!
-
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Microbiology HTL
Klebsiella
Klebsiella
pneumoniae (K.
oxytoca or
Friedlanders bacilli)
short, Gram (-) , non-motile, posess thick
capsule of
polysaccharide,facultative
anaerobes, killed at
55 degrees
http://rds.yahoo.com/S=96062857/K=klebsiella/v=2/SID=w/l=II/R=2/SS=p/OID=c1969b27ca0dc6a8/SIG=1gv3mjicp/EXP=1122535421/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dklebsiella%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=432&w=576&imgcurl=www.gcarlson.com%2Fimages%2FKlebsiella-2reduced.jpg&imgurl=www.gcarlson.com%2Fimages%2FKlebsiella-2reduced.jpg&size=77.9kB&name=Klebsiella-2reduced.jpg&rcurl=http%3A%2F%2Fwww.gcarlson.com%2Fmicrobial_krebsiella.htm&rurl=http%3A%2F%2Fwww.gcarlson.com%2Fmicrobial_krebsiella.htm&p=klebsiella&type=jpeg&no=2&tt=332&ei=UTF-8 -
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Microbiology HTL
Cultural char.:
large, mucoidcolonies, tenaceous
colonies, repeated
culture loses capsule
Biochemical:
ferment lactose,
sucrose, maltose,
mannitol & salicin
IMViC - --++
http://rds.yahoo.com/S=96062857/K=klebsiella/v=2/SID=w/l=II/R=3/SS=p/OID=c67c17784c488a5a/SIG=1kdvfngho/EXP=1122535421/*-http%3A//images.search.yahoo.com/search/images/view?back=http%3A%2F%2Fimages.search.yahoo.com%2Fsearch%2Fimages%3Fp%3Dklebsiella%26ei%3DUTF-8%26fr%3DFP-tab-img-t-283%26fl%3D0%26x%3Dwrt&h=400&w=500&imgcurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FKlebsiella%2520pneumoniae.jpg&imgurl=skywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2FKlebsiella%2520pneumoniae.jpg&size=15.3kB&name=Klebsiella%20pneumoniae.jpg&rcurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&rurl=http%3A%2F%2Fskywind.wkhc.ac.kr%2F%25B9%25CC%25BB%25FD%25B9%25B0%2Fmicrow.htm&p=klebsiella&type=jpeg&no=3&tt=332&ei=UTF-8 -
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Microbiology HTL
Pathogenesis: causes
bacterial pneumonia,enteritis, Pharyngitis,
sinusitis, septicemia,
meningitis,
peritonitis, UTIAntigenic structure:
72 capsular types
Type 1&2 -respiratory
Type 8,9,10 - UTI
http://rds.yahoo.com/S=96062857/K=klebsiella/v=2/SID=w/l=II/R=15/SS=p/OID=7cd22e257dfb683e/SIG=1ikiqarbl/EXP=1122535421/*-http%3A//images.search.yahoo.com/search/i