microbiology (bacteriolog) lab - practicals 1

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Scientific Name: Corynebacterium diphtheriae Common name: Kleb-Loeffler’s bacillus Morphology: irregular bacilli with Babes-Ernst bodies club shaped, w/ barbed ends V, X, Y or Chinese character arrangements Gram Reaction: Gram positive (+) Staining method: Gram stain Scientific Name: Bacillus subtilis Morphology: bacilli in chains Gram Reaction: Gram positive (+) Staining method: Gram stain Scientific Name: Bacillus subtilis Morphology: bacilli in chains Gram Reaction: Gram positive (+) Staining method: Gram stain Scientific Name: Bacillus subtilis Morphology: bacilli in chains Gram Reaction: Gram positive (+) Staining method: Gram stain Scientific Name: Bacillus subtilis Common name: Hay bacillus Morphology: bacilli in chains Special Structure: spores (color green) Vegetative cells (color red) Staining method: Fulton Schaeffer method Scientific Name: Vibrio cholerae Common name: Comma bacillus Morphology: curved bacilli, singly; comma shaped Gram Reaction: Gram negative (-) Staining method: Gram stain

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University of Santo TomasFaculty of Pharmacy

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Scientific Name: Corynebacterium diphtheriae

Common name: Kleb-Loeffler’s bacillus

Morphology: irregular bacilli

with Babes-Ernst bodies

club shaped, w/ barbed ends

V, X, Y or

Chinese character arrangements

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Bacillus subtilis

Morphology: bacilli in chains

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Bacillus subtilis

Morphology: bacilli in chains

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Bacillus subtilis

Morphology: bacilli in chains

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Bacillus subtilis

Common name: Hay bacillus

Morphology: bacilli in chains

Special Structure: spores (color green)

Vegetative cells (color red)

Staining method: Fulton Schaeffer method

Scientific Name: Vibrio cholerae

Common name: Comma bacillus

Morphology: curved bacilli,

singly; comma shaped

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Escherichia coli

Common name: Colon bacillus

Morphology: bacilli singly

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Escherichia coli

Common name: Colon bacillus

Morphology: bacilli singly

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Klebsiella pneumoniae

Morphology: bacilli in pairs

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Pseudomonas aeruginosa

Morphology: short bacilli singly

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Bacillus subtilis (+),

Escherichia coli (-)

Morphology: bacilli in chains,

bacilli singly

Gram Reaction: Gram positive (+)

Gram negative (-)

Staining method: Gram stain

Scientific Name: Klebsiella pneumoniae

Morphology: bacilli in pairs

Special Structure: capsule (not stained)

Staining method: India ink stain

(a negative or relief staining method)

(Background is stained black)

Scientific Name: Mycobacterium tuberculosis

Common name: Tubercle bacilli

Synonyms: Koch’s bacillus

Morphology: slender bacilli

Acid Fast Reaction: Acid fast

Staining method: Acid fast stain

Scientific Name: Mycobacterium tuberculosis

Common name: Tubercle bacilli

Synonyms: Koch’s bacillus

Morphology: slender bacilli

Acid Fast Reaction: Acid fast

Staining method: Acid fast stain

Scientific Name: Streptococcus pneumoniae

Morphology: cocci in pairs

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Spirillum volutans

Morphology: spiral shaped, singly

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Streptococcus pneumoniae

Morphology: cocci in pairs

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Streptococcus pyogenes

Morphology: cocci in chains

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Neisseria gonorrhoeae

Common name: Gonococci

Morphology: cocci in pairs

Gram Reaction: Gram negative (-)

Staining method: Gram stain

Scientific Name: Gaffkya tetragena

Morphology: cocci in groups of 4

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Sarcina lutea

Morphology: cocci in groups of 8

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Sarcina lutea

Morphology: cocci in groups of 8

Gram Reaction: Gram positive (+)

Staining method: Gram stain

Scientific Name: Staphylococcus aureus

Morphology: cocci in clusters

Gram Reaction: Gram positive (+)

Staining method: Gram stain

BRUCAL, J.C.L.