microbial metabolism
DESCRIPTION
LECTURE 10:. Microbial Metabolism. Microbiology and Virology; 3 Credit hours Atta- ur - Rahman School of Applied Biosciences (ASAB) National University of Sciences and Technology (NUST). ATP. Interesting Fact. - PowerPoint PPT PresentationTRANSCRIPT
Microbial Metabolism
LECTURE 10:
Microbiology and Virology; 3 Credit hours
Atta-ur-Rahman School of Applied Biosciences (ASAB)National University of Sciences and Technology (NUST)
ATP
Interesting Fact
• ATP is continuously recycled in organisms: the human body, which on average contains only 250 grams of ATP, turns over its own body weight equivalent in ATP each day.
• The collective metabolic capacities of bacteria allow them to metabolize virtually every organic compound on this planet.
Substrate level phosphorylation
Sugar Metabolic Network in Bacteria
Amphibolic Pathway
THE BREAKDOWN OF GLUCOSE TO PYRUVATE
• Microorganisms employ several metabolic pathways to catabolize glucose and other sugars– The Embden Meyerhof pathway, – The pentose phosphate pathway, and– The Entner Doudoroff pathway
• These three pathways are collectively called as glycolytic pathways or as glycolysis
Glycolytic Pathway
Stage 1
Stage 2
The
Embd
en M
eyer
hof p
athw
ay
Stage 1
Triose phosphate isomerase
Glyceraldehyde 3 phosphate dehydrogenase
Phospho glycerate kinase
Phosphoglycerate mutase
Enolase
Pyruvate Kinase
Stage 2
The Pentose Phosphate Pathway
The Pentose Phosphate Pathway Importance in Biosynthesis
• NADPH from the pentose phosphate pathway serves as a source of electrons for the reduction of molecules during biosynthesis
• Two important precursor metabolites:– erythrose 4-phosphate, which is used to synthesize
aromatic amino acids and vitamin B6 (pyridoxal) – and ribose 5-phosphate which is a major component of
nucleic acids.• Hexose sugars needed for peptidoglycan synthesis
The Entner Doudoroff Pathway
KDPG aldolase
This pathway yields one ATP, one NADPH, and one NADH per glucose metabolized