microbial identificationstate-of-the-science performancewith unmatched power & versatility

(Microbial ID Products Not For Human In Vitro Diagnostic Use)

Biolog’s latest generation redox chemistry enables testing and identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed. A simple, one minute setup protocol is used for each sample. e expanded GEN III database is designed to meet the needs of Biolog’s broad customer base covering diverse disciplines of microbiology.

All Biolog Microbial Identification Systems — manual, semi-automated or fully-automated — use the powerful GEN III MicroPlate™, allowing users to determine the most appropriate system to fit their current budget and level of throughput. Should needs change, all systems can be upgraded and expanded to meet new capacity requirements.

Revolutionary GEN III

microbial identificationGEN

III

• NO Gram stain• NO pre-tests• NO follow-on tests• ONE panel for both GN & GP bacteria• ONE minute set-up• OVER 2900 species coverage

Biolog's single panel is easy to use, and identifies 4 times more species than alternative systems

e GEN III redox chemistry is applicable to an unprecedented range of both Gram-negative and Gram-positive bacteria. GEN III dissects and analyzes the ability of the cell to metabolize all major classes of biochemicals, in addition to determining other important physiological properties such as pH, salt, and lactic acid tolerance, reducing power, and chemical sensitivity.

One technology with multiple platforms, for consistent performance across every level of your organization.

3rd generation identification technologywith breakthrough advantages

NaCl

Lactic acid

Reducing power

GN-GP

Acidic pH

Sugars

Hexose-PO4’s

Amino acids

Hexose acids

Carboxylic acids,esters, and fatty acids

71 Carbon Source plus 23 Chemical Sensitivity Assays

Anatomy of a GEN III identification.ID = Stenotrophomonas maltophilia

Biolog’s powerful carbon source utilization technology accurately identifiesenvironmental and pathogenic microorganisms by producing a characteristicpattern or “metabolic fingerprint” from discrete test reactions performed within a96 well microplate. Culture suspensions are tested with a panel of pre-selected assays,then incubated, read and compared to extensive databases of environmentalorganisms, human pathogens, veterinary pathogens and plant pathogens. e scopeof the 96 assay reactions, coupled with sophisticated interpretation soware, deliversa high level of accuracy that is comparable to molecular methods. e one minuteper sample set up is much simpler and faster than DNA sequencing and theautomated pattern matching eliminates the need for training and expertise in genesequence interpretation.

microbial identification...with the OmniLog ID System

AUTOMATED

e OmniLog® ID System offers a simple automated process to meet the dynamic workflow demands of a wide range of microbiology applications. Even organisms requiring special incubation temperatures or environments are easily accommodated, ensuring accurate identification for a broad spectrum of Gram-negative and Gram-positive bacteria.

e OmniLog ID System fully automates the process of microbial identification by incubating, reading and interpreting results from up to 50 Biolog MicroPlates™ at a time. e system soware provides directives to guide all steps of the testing process for each MicroPlate, including sample placement and removal and extended incubation when needed. Additional MicroPlates can be loaded and processed any time there is an open tray or identification has been completed on a previously entered MicroPlate. Up to date information for each MicroPlate, including results as they become available, are continuously visible on the system’s menu screen. Identifications take as little as 2 hours.

e OmniLog Plus ID System adds testing capabilities for anaerobic bacteria, yeasts, and filamentous fungi. Both OmniLog systems allow results to be saved to create a customized database or to track specific organism characteristics and frequency of isolation. e system soware, which includes the RetroSpect™ soware module for organism tracking and trending, is extremely easy to navigate, and provides all the required functionality to support 21CFR Part 11 electronic record requirements and regulatory compliance. Administrative features are also available to control operator access and the creation or modification of data files.

full automation to meetthe demands of a dynamic workflow

e MicroStation™ ID System is a versatile system, with the ability to identify and characterize a wide range of environmental and pathogenic organisms across diverse fields of microbiology. Using all Biolog databases, over 2650 species of bacteria, yeast and filamentous fungi can be identified. Some bacteria can be identified in as little as 2 hours. Just prepare a cell suspension and inoculate the appropriate MicroPlate.

Aer inoculation and incubation, the MicroPlate is placed into the MicroStation Reader for analysis. e unique metabolic pattern generated by the organism is recorded and compared to hundreds of identification profiles in a corresponding Biolog Database. e versatile plate reader uses dual wavelength readings to quantify color reactions in the MicroPlate wells, adding consistency and accuracy when reading the reaction patterns.

Biolog’s patented redox chemistry makes use of different carbon compounds including sugars, carboxylic acids, amino acids and peptides to provide an unparalleled wealth of discriminating biochemical characterizations. is diverse set of tests enables our systems to identify microorganisms that other kit-based methods misidentify or fail to identify. e MicroStation System, as well as the OmniLog System, has extensive applications also for microbial community analysis in soil, water, biofilms and other environments.

Over 2900 species of bacteria, yeast and filamentous fungi can be identified

microbial identification...with the MicroStation ID System

SEMI AUTOMATED

Building on Powerful Data

RetroSpect is a specialized soware tool that supports data management and reporting and provides sophisticated trending and tracking of microbial data. Each Biolog MicroPlate provides an extensive data set of strain specific test information from each isolate. e new RetroSpect Soware utilizes these data elements in a powerful analysis package. Data Management filters enable users to define the content of the databases and generate highly customized and informative reports.

Isolate Prepare Inoculate Incubate and Read

Rewarding our customers with innovation and greater performance for more than 30 years

unmatched versatilitythat includes pragmatic tools

Isolate Prepare Inoculate Incubate and Read

Accurate identifications in any setting.Biolog’s MicroLog® manual microbial ID system is the ideal choice for testing environments with lower volume requirements or budget constraints. e system offers basic identification capability for aerobic Gram-negative and Gram-positive bacteria. GEN III MicroPlate results are read visually and entered into the MicroLog Soware’s data entry screen for analysis and organism identification. is System includes a turbidimeter, the MicroLog soware and electronic user guide. Databases, RetroSpect software and electronic pipettor are sold separately allowing users to customize the system to meet their organization’s individual needs.

A simple, straightforward procedure.1. Isolate a pure culture on agar media2. Prepare inoculum at specified cell density3. Inoculate the Biolog MicroPlate4. Incubate the plate, observe and enter the reaction pattern to obtain ID result

microbial identificationwith the MicroLog ID System

MANUAL

Only Biolog technology provides users with both anidentification AND a detailed strain characterization.

the convergence of two powerful technologieswith the OmniLog Combo Systems

COMBO

e OmniLog® Combo Systems provide the microbiologist with a unique solution for bothmicrobial identification and characterization of cellular functions in a single system. eycombine the power of both of Biolog’s Microbial Identification and Phenotype MicroArray testingplatforms. ese capabilities are ideal for pursuing a wide range of studies, from routine species-level identification to projects in detailed strain characterization, such as fingerprinting andtracking strains that cause product contamination, comparison of nonpathogenic and pathogenicstrains, gene and mutant characterization, cell line quality control, and bioprocess improvement.Both platform technologies employ Biolog's patented colorimetric redox chemistry to detectcellular energy production and Biolog’s computer-controlled detection instrumentation.

Phenotype MicroArray™ TechnologyPhenotype MicroArray technology uses the same chemistry and format, but tests a much larger andmore diverse set of cellular properties. It enables researchers to evaluate nearly 2000 phenotypes ofa microbial cell in a single experiment. With automated instrumentation, phenotypic propertiescan be measured quantitatively and kinetically, then recorded automatically into electronic records.rough comprehensive and precise quantitation of phenotypes, researchers are able to obtain anunbiased perspective of the effect on cells of genetic differences, environmental change, and exposureto drugs and other chemicals. ey can correlate genotypes with phenotypes, determine a cell’smetabolic and chemical sensitivity properties, discover new targets for antimicrobial compounds,optimize cell lines and culture conditions in bioprocess development, characterize cell phenotypesfor taxonomic or epidemiological studies, and more.

ORDERING INFORMATION

ORDER INFORMATIONCat #91371

91391

DescriptionOmniLog ID System, for rapid automated incubation and identification of aerobic bacteria. Includes OmniLog incubator/reader, OmniLog GEN III Data Collection Soware, RetroSpect Data Management Soware, electronic user guide, computer, monitor, printer, pipettor, turbidimeter, web based training and a one year warranty. Databases are sold separately.

OmniLog Plus ID System, for rapid automated incubation and identification of aerobic bacteria, plus rapid identification of anerobic bacteria, yeast, and filamentous fungi. Includes OmniLog incubator/reader, OmniLog GEN III Data Collection Software, RetroSpect Data Management Software, MicroStation Software, electronic user guide, computer, monitor, printer, pipettor, turbidimeter, web based training and a one year warranty. Databases are sold separately.

ORDER INFORMATIONCat #65361

DescriptionMicroStation ID System, for semi-automated identification of bacteria, yeast, and filamentous fungi. Includes MicroStation reader, MicroStation GEN III Data Collection Soware, RetroSpect Data Management Soware, electronic user guide, computer, monitor, pipettor, turbidimeter, web based training and a one year warranty. Databases and printer are sold separately.

MICROSTATION SPECIFICATIONSSize: 15.5 in (39.4 cm) w | 8.75 in (22.2 cm) h | 16 in (40.6 cm) d

Weight: 30 lbs. (13.6 kg)

Power: 100 to 240 volts, 50 to 60 Hz

Operating Temp Range: 18° to 28° C

Incubation Temperature Range: 4° C above ambient to 50° C

Temp Consistency: ±0.5° C in the tray chamber

Test Capacity: 1 microplate

Reading Cycle Time: 15 mins

Detection Method: Absorbance

Read Method: Endpoint, kinetic, linear scanning

Monitor: 22” LCD flat panel

For ordering information on Biolog consumables (MicroPlates, fluids, reagents, biologicals), accessories, Preventive Maintenance Programs & Service Plans, and On-Site Validation Services, consult your local Biolog representative

microbial identificationunequaled versatility and accuracy

AUTOMATED

SEMI AUTOMATED

OMNILOG INCUBATOR SPECIFICATIONSSize: 21 in (53 cm) w | 32 in (81 cm) h | 23 in (58 cm) d Power:

100 to 240 volts, 50 to 60 Hz

Operating Temp Range: 18° to 28° C

Operating Humidity Range: 20% to 80% non-condensing

Incubation Temperature Range: 22° to 45° C

Temp Consistency: ±2° C in the tray chamber

Incubation Humidity Range: Ambient

Test Capacity: 50 microplates

Reading Cycle Time: Up to 25 trays with 50 microplates in 15 mins

Temperature Control: Input of set temperature by external computer

Temperature Indication: Output to external computer and 7 segment

display (green)

Other Indicators: Power On (green), Interrupt On (red), and Not

at Temperature (red)

Monitor: 22” LCD flat panel

for Biolog's diverse customer basein a wide range of applications

ORDER INFORMATIONCat #45101

DescriptionMicroLog M System, for identification of aerobic bacteria based on visual inspection and manual entry of results. Includes MicroStation GEN III Data Collection Soware, electronic user guide, turbidimeter, and a one year warranty. Databases, RetroSpect Data Management Soware and multichannel pipettor are sold separately.

ORDER INFORMATIONCat #94371

94391

DescriptionOmniLog Combo ID System, for rapid automated incubation and identification of aerobic bacteria. Also includes t he capability for Phenotypic analysis of cells. Includes OmniLog incubator/reader, OmniLog GEN III Data Collection Soware, RetroSpect Data Management Soware, Phenotype MicroArray Soware, electronic user guide, computer, monitor, printer, pipettor, turbidimeter, web based training and a one year warranty. Databases f or microbial identification are sold separately.

OmniLog Combo Plus ID System, for rapid automated incubation and identification of aerobic bacteria, plus rapid identification of anerobic bacteria, yeast, and filamentous fungi. Also includes the capability for Phenotypic analysis of cells. Includes OmniLog incubator/reader, OmniLog GEN III Data Collection Soware, RetroSpect Data Management Soware, Phenotype MicroArray Soware, MicroStation, MicroStation Soware, electronic user guide, computer, monitor, printer, pipettor, turbidimeter, web based training and a one year warranty. Databases for microbial identification are sold separately.

ORDER INFORMATIONCat # Description22730D GEN III Database, for both Gram-negative & Gram-positive bacteria. Includes appropriate Turbidity Standards.

22607D AN Database, for Anaerobic bacteria identification. Requires MicroStation or OmniLog Plus System.

22605D YT Database, for Yeast identification. Requires MicroStation or OmniLog Plus System.

22606D FF Database, for Filamentous Fungi identification. Requires MicroStation or OmniLog Plus System.

26112D RetroSpect Data Management Soware, for MicroLog M System. (included with other systems)

MANUAL

COMBO SYSTEMS

DATABASES AND SOFTWARE

one technology with multiple platforms,for high level performance in laboratoriesof all interests, sizes and budgets

All Biolog Microbial Identification Systems—manual, semi-automated or fully-automated—are based on the same powerful technology, allowing users to determine the most appropriate systemto fit their current budget and level of throughput. And should needs change, all systems can beupgraded and expanded to meet new capability or capacity requirements.

All Biolog microbial testing is performed in a convenient dry panel MicroPlate format. A cellsuspension is added and the resulting metabolic pattern or “fingerprint” generated by themicroorganism is recorded and compared to hundreds of identification profiles in a correspondingBiolog Database. Databases are periodically updated to reflect the newest taxonomy, incorporateadditional species, and extend additional capabilities to meet the growing needs of Biolog’s diversecustomer base.

Biolog products and technical support are available all over the world. For more information,contact Biolog or your local authorized Biolog representative, or visit our website at www.biolog.com

BIOLOG, Inc.21124 Cabot Blvd, Hayward, CA 94545 USA (510) 785-2564 • www.biolog.com

Part # 00A024, Rev D 09/2019 • © Biolog Inc. 2019