microbial diversity & pseudomonas aeruginosa · jennifer rattray, sam brown lab @ gt...
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Microbial Diversity & Pseudomonas aeruginosa Jennifer Rattray, Sam Brown Lab @ GT
@jenniferbethann
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Phylogenetic classification of micro-organisms
Universal Ancestor
Algae Fungi Protozoa
Prokaryotic
Eubacteria Archaeabacteria
Eukaryotic
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Human hair ~75µM
Pseudomonas aeruginosa, ~1-2 µM
Small Organisms, Big Impacts
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Small Organisms, Big Impacts
4inno, Mekhala Raghavan
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Small Organisms, Big Impacts
USGS SIR 2004-5144
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Small Organisms, Big Impacts
UGA Physics & Astronomy
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Small Organisms, Big Impacts
Bäckhed et al. 2013
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Culture CharacteristicsBacteria can grow on… anything!
Liquid Biofilm Aggregate
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MetabolismTake in nutrients from the environment glucose, lactose, other sugars, fats=lipids, proteins, toxic wastes, oils and petrol
Assimilate the nutrients into BIOMASS DNA, proteins, carbohydrates and complex carbohydrates, lipids
Release waste products into the environment gases, alcohols, acids and organic compounds
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Differentiation— to form distinct structures
Bacillus spp. endospore forming cells Anabaena spp. Cyanobacteria forming heterocysts
T.J. Deveridge M. Dworkin
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Communicationinteraction withother cells—response to other cellsVibrio fischeri and Lantern fish
Kolter and Losick
AMNH--NYC
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Genotype – how genes code for a specific trait
Phenotype – how the organism acts (some ambiguity within the field)
Morphotype – physical appearance of cells/colonies
Vocab. Distinction
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Phenotypic diversity
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Pseudomonas aeruginosaConsidered “ubiquitous” in nature
Opportunistic pathogen - Disruption of mucous membrane/skin - Intravenous catheters & urinary catheters & cancer therapy - Burn wound patients
Incredibly diverse! High levels of phenotypic diversity.
Inherently resistant to many antibiotics and can evolve resistance to more readily.
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Color – pyocyanin, pyoverdine, pyorubin
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Collective Behaviors of Bacteria
Group dependent & group beneficial
behaviors
https://sites.tufts.edu/quorumsensing/quorumsensing101/
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Niche (Nick)
Environmental 30Host 39Cystic Fibrosis 5Other 2
Sub-Niche (Jennifer)
Infection/Clinical 26Chronic 5Host Associated 14Fresh Water 10Sea Water 12
Deep Sea Water 2Surface Associated 5
Perron Isolate Library
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Phenotypes
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Morphotypes
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Inter-colony variation
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Can we get any information about phenotype from morphotype?Can we make medically relevant assumptions?
Goal:
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Intra-colony variation
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PIL 129-3
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