microarray background
DESCRIPTION
Microarrays – A New Revolution: Genomic Expression Analysis of Oxidative Stress Genes in Saccharomyces cerevisiae Dana Arico Betsy Karle Jeff Kucharski Kumo Lartevi Dr. Terrie Rife. Microarray Background. Allows researchers to analyze the expression of thousands of genes at one time. - PowerPoint PPT PresentationTRANSCRIPT
Microarrays – A New Revolution: Genomic Expression Analysis of
Oxidative Stress Genes in Saccharomyces cerevisiae
Dana AricoDana AricoBetsy KarleBetsy Karle
Jeff Kucharski Jeff Kucharski Kumo Lartevi Kumo LarteviDr. Terrie RifeDr. Terrie Rife
Microarray Background
•Allows researchers to analyze the expression of thousands of genes at one time.
•This technique can be used to identify and analyze mutations in DNA
•Model organisms, whose genome has been completely sequenced, are used in microarray experiment.
First Microarray Experiment
• Was performed by researchers from the NIH, Stanford University, and others
• Wanted to compare gene expression of patients with B-cell Lymphoma
• Patients fell under two categories: • Long-term survivors
• Short-term survivors
Preparing the DNA Chip
cDNA
Arrayed
Genes
Robotic
Printing
Cloning &
Sequencing
Preparing the Samples
Long-term Survivors / Short-term Survivors
Extraction
RNA
cDNA &
Labeling
RT reaction
The Whole Picture
Arrayed
Genes
Labeled
cDNA
Hybridized
Array
Hybridization
Gene Expression
Long-term survivors Short-term survivors
1) I 1)
2) II 2) II
3) 3) I
I
II II
II
I
I
Thousands of Genes