metagenomics: applied to a broad range of environments · metagenomics: applied to a broad range of...
TRANSCRIPT
Metagenomics: applied to a broad range of environments
DNA
extraction
Sequencing
mRNA
extraction
PCR
AmpliconMetatranscriptome Metagenome
A free to use resource for the archiving,
assembly, analysis, & browsing of
microbiome data
AnalysisData archiving Assembly
Assembly
Binning
Reference based and de novo
Different way to analyse shotgun metagenomics
Amplicon
& raw read analysis
• Recent advances
• Increased sequencing depth
• Reduced chimeric sequences
• Reduced computational cost
••
•
••
7
$ mg-toolkit sequence_search -s my.fasta
Nature 2018 Jun;558(7711):595-599