meningitis: the smooth criminal
DESCRIPTION
Trevor Kraeger, Tyler Stokka, Jordan Williams, Chris Werner, Kassidy Branning, Anna Grim, Ben Weber, Emily Mytinger, Kaitlyn Rothamer, and GBH! SMART Team Project 2011. Meningitis: The Smooth Criminal. Common Cold or Something Worse???. Fever Persistent headaches Vomiting - PowerPoint PPT PresentationTRANSCRIPT
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Meningitis: The Smooth Criminal
Trevor Kraeger, Tyler Stokka, Jordan Williams, Chris Werner, Kassidy Branning, Anna Grim, Ben Weber, Emily Mytinger, Kaitlyn Rothamer, and GBH!
SMART Team Project 2011
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Common Cold or Something Worse???
Fever
Persistent headaches
Vomiting
Stiff and painful neck
Seizures
Skin rash
Dizzy spells
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Statistics of Meningitis
1,500 infected annually
900-3,000 reported cases annually
Occurs in adolescents and young adults
1 out of every 7 adolescents will die
Are you caught up on your vaccinations???
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What Exactly is Meningitis???
Swelling of the meninges
Bacterial and viral
Viral more common
Bacterial is deadly
Outbreaks prevalent in close quarters
Permanent effects
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Meningitis Across the Globe
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A Little Closer To Home
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Neisseria meningitidis
Gram Negative bacteria
Has an extra cell membrane
Thinner peptidoglycan layer
More complex (two zones rather than one)
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Gram Negative: Why it is important
Evade and Invade
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How does this happen???
Penetration of the blood brain barrier (BBB)…
…through the help of heparin, fibronectin and OpcA
Secret Agent Analogy
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via pilius interactions, OpcA
opca interactions with the heparin and fibronectin or vitronectin
another Heparin sulfate proteoglycan receptor method
Three different ways to
Get Into the Cell
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The 3 Major Proteins Key to Infiltration
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“Arm” of bacterial Meningitis
Has a polar positive region in the notch on the “front”
Mainly consists of a Beta Barrel
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A component of Blood serum
a highly-sulfated long unbranched polysaccharide
an anticoagulant
Highest negative charge density of any known biological molecule!
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2 Heparin Binding Sites
Integrin Binding site!
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Meningitis Passing through Unnoticed
OpcA positive groove binds to negative Heparin
Heparin bind to positive fibronectin sites (two sites)
Fibronectin attaches to the integrin on the exterior of the blood-brain barrier
Receptor Mediated Endocytosis
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How to Prevent Meningitis from penetrating the BBB
Methods of prevention
OpcA positive groove
Nothing is successful yet
With the technology of today currently expanding it will only be a matter of time before a mechanism is found.
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S.M.A.R.T. Team
Students
Modeling
A
Research
Topic
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Our Mission…To perform background research on how meningitis works.
To model the proteins involved using JMOL
To give a kick butt presentation on meningitis and how it infiltrates the BBB!
After the above 3 objectives are completed, report back to GBH for your next assignment.
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Not as Easy as You Think
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Starting from Scratch
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X-ray Crystallography
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XYZ Data: Caterpillar to Butterfly
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Protein Data Bank
Scientists make the protein data available to the public.
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Acknowledgments Dr. Shannon Colton
MSOE/ Center for Biomolecular Modeling
SMART Team Program Other Smart team groups at DCE
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Oh…btw…
…WE FOUND WALDO!!!
You probably didn’t notice him up there during the entire presentation, did ya???