mechanism of signal transduction by rhodopsin as a model gpcr by basak isin
TRANSCRIPT
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Mechanism of Signal Mechanism of Signal
Transduction by Transduction by
Rhodopsin as a Model Rhodopsin as a Model
GPCRGPCR
by by Basak IsinBasak Isin
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OUTLINEOUTLINE
G Protein-Coupled-Receptors (GPCR)G Protein-Coupled-Receptors (GPCR) Aim of the projectAim of the project Gaussian Network Model (GNM)Gaussian Network Model (GNM) Anisotropic Network Model (ANM)Anisotropic Network Model (ANM) Results and DiscussionResults and Discussion Conclusion and SummaryConclusion and Summary Future PlansFuture Plans
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GPCRsGPCRsthe largest superfamily of cell surface receptorsthe largest superfamily of cell surface receptors
seven helices - their signature motif seven helices - their signature motif involved in a number of clinically important ligand/receptor processes. bind ligands from the cell exterior, which induce a conformational change in the cytoplasmic face of the receptor, enabling binding of the G protein. couple to heterotrimeric G proteins to convert an extracellular signal into an intracellular signal.significant drug targets. 50-60% of approved drugs target members of the GPCR family.
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RHODOPSINRHODOPSINthe first 3-dimensional molecular model for a GPCRthe first 3-dimensional molecular model for a GPCR
1
2 3
4
65
cytoplasmic region
extracellular region
7
8
located in the outer segments of rod photoreceptor cells in the retinaresponds to environmental signals, i.e., photons initiates intracellular processes that result in an electrical signal
processed by the visual system.
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hCOOH
S-S-
VII
IVI
IIIV
V
NAc
VI V
VII
K
+
VI
III
COOH
S-S-
K
VII
IVI
IIIV
V
NAc
K
VI V
VII
III
Sensitization
Desensitization
LIGHT ACTIVATIONLIGHT ACTIVATION
11-cis retinal All-trans retinal
Metarhodopsin IIRhodopsin
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Glycosylation at 2 asparagines 2 Cys(110/187) form sulfide bridgeLys296 at H7 is covalently attached to
11-cis retinal (protonated Schiff base) Glu113 at H3 is the counterion to Schiff
basePalmitate attached to 2 C-terminal CysSer334, 338 & 343 are major sites for
phosphorylation
SNAKE LIKE SNAKE LIKE REPRESENTATIONREPRESENTATION
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the the Asp/Glu Arg Tyr (ERY)Asp/Glu Arg Tyr (ERY) motif at motif at the cytoplasmic end of the cytoplasmic end of helix 3helix 3
the the Asn Pro X X TyrAsn Pro X X Tyr (NPXXY)(NPXXY) motif motif in in helix 7helix 7 (Okada et al., 2002) (Okada et al., 2002)
the the X1BBX2X3BX1BBX2X3B motif at motif at cytoplasmic end of cytoplasmic end of helix 6helix 6 (B, basic; (B, basic; X, non-basic) (Ballesteros et al., X, non-basic) (Ballesteros et al., 1998),1998),
an ionic interaction between the an ionic interaction between the ligand and the receptor at the ligand and the receptor at the retinal retinal Schiff base Lys296Schiff base Lys296 and and the Schiff the Schiff base counterion the Glu113base counterion the Glu113 (Cohen et al., 1993),(Cohen et al., 1993),
the the Asn-AspAsn-Asp interaction between interaction between helices 1 and 2helices 1 and 2, respectively, respectively
the aromatic cluster surrounding the the aromatic cluster surrounding the ligand binding pockets (Visiers et al., ligand binding pockets (Visiers et al., 2002)2002)
MicrodomainsMicrodomains
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AIM OF THE PROJECTAIM OF THE PROJECT
Understanding the mechanism of
activation of rhodopsin as a model
GPCR and its interaction with Gt by
structure-functions analysis using the
GNM and its extension, the ANM.
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Gaussian Network ModelGaussian Network Model (GNM)(GNM)
For estimating the dynamic characteristics of For estimating the dynamic characteristics of
biomolecular structures based on atomic biomolecular structures based on atomic
coordinates in the native conformationcoordinates in the native conformation
Elastic network Virtual bond representationNo distinction between nonbonded and bonded neighborsThe interactions between residues in close proximity represented by harmonic potentials with a uniform spring constant
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CONSTRUCTION OF CONSTRUCTION OF KIRCHHOFFKIRCHHOFF MATRIXMATRIX
5
1 2 3 4
18
19
2021
22
238
3
1 2 3 4……18 19 20 21….N
-1 -1 -1 -1…..-1 -1 -1…-1….0
7A
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= U UT -1 = U -1UT
The fluctuations associated with kth mode
U: orthogonal (NxN) matrix
uk: kth eigenvector of Γ (1 ≤ k ≤ N) (shapes of corresponding mode of motion)
: diagonal matrix with eigenvalues (k)
1 = 0 <2 < …< N frequency of modes
= (3kBT/ ) (k-1[uk]i [uk]j )
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the mechanism of the motion relevant to biological function.the mechanism of the motion relevant to biological function.
The maxima of the slow mode curves indicate the most flexible regions of the The maxima of the slow mode curves indicate the most flexible regions of the
molecule. molecule.
Identifies the hinge region that are important for biological function.Identifies the hinge region that are important for biological function.
Slow Modes Global Motions
a
222-230
6-11
27-33
26-37
14-20
cb d
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Comparison of theoretically calculated all Comparison of theoretically calculated all modes with B- factors found by X-ray modes with B- factors found by X-ray
crystallographycrystallography
RESULTS AND DISCUSSION
Science, Palcwezski et. al, 2000
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First Mode of GNMFirst Mode of GNM
0
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
0.01
0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340
Residue
Dis
tribu
tion
of F
luct
uaio
ns
H1
H2H3 H4
H5 H6 H7
12
76
53
The color codes are green, cyan, blue, magenta, pink and yellow in the order of
increasing mobility.
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CROSS CORRELATIONCROSS CORRELATIONOf GNMOf GNM
Res
idue
num
ber
Residue number
a
Residue number
Residue number
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Fast ModesFast ModesCritically important ones for the
overall stability of the molecule and
evolutionarily conserved
High Frequency ModesHigh Frequency Modes
Asn55
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ANMANMthe three components of the inter-residue separation vectors obey the three components of the inter-residue separation vectors obey
Gaussian dynamics.Gaussian dynamics.involves the inversion of a 3N x 3N Hessian matrix involves the inversion of a 3N x 3N Hessian matrix HH that replaces the N that replaces the N
x N Kirchhoff matrix x N Kirchhoff matrix (Doruker (Doruker et al., et al., 20002000,, Atilgan Atilgan et al.,et al., 2001). 2001).
ANM results
Front View Back View
ANM results
Wild type
ab
17
6 3 5
28
8
1
8
5
6
241
72
63
5
12
34
6
5
7
7
4
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MOVIESMOVIES
FRONTFRONT
BACKBACK
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TWO CONFORMATIONSTWO CONFORMATIONS
1 2 34
55
678
4
321
678
b 1
11
2 22
33
3
4
4 4
55 5
6 6 67 7 7
8 8 8
wild type ANMbANMa
ANMa MM(199): E=-5531.2ANMb MM(273): E=-5235.41
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Cross-sections from Cross-sections from the Topthe Top
Top-248
248-255
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255-263
264-273
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bottom
273-277
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RRijij
H1
H2
H3
H4
H5
H6
H7H8
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RETINAL REGIONRETINAL REGION
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ALL ATOM MODELALL ATOM MODEL
•Adding the fluctutations of C to every atom in the PDB structure•Energy minimize the structure
•to see the side chain motions•to study the microdomains
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E-MINE-MIN
NO RETINAL CIS-RETINAL TRANS-RETINAL
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MICRODOMAIN1-ERYMICRODOMAIN1-ERY
ANM ANM WILD TYPEWILD TYPE
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Wild Type 11-Trans Wild Type 11-Trans RetinalRetinal
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CONCLUSIONCONCLUSION The rhodopsin ground state structure is predisposed for the functional The rhodopsin ground state structure is predisposed for the functional
conformational changes leading to the opening of the helical bundle, conformational changes leading to the opening of the helical bundle, thereby revealing the mechanism for this process.thereby revealing the mechanism for this process.
The mechanism for the observed opening of the helical bundle is The mechanism for the observed opening of the helical bundle is mediated by mediated by a torsional rotation of the molecule centered on Helix 3a torsional rotation of the molecule centered on Helix 3. .
The cytoplasmic end of helix 4 moves away from the cytoplasmic end of The cytoplasmic end of helix 4 moves away from the cytoplasmic end of helix 3, the most flexible region in helix 3, and stretches the helix 3, the most flexible region in helix 3, and stretches the cytoplasmic loop 2. cytoplasmic loop 2.
Helix 6 is rotates while simultaneously elongating, comparable to a Helix 6 is rotates while simultaneously elongating, comparable to a turning screw. turning screw.
Furthermore, they suggest a mechanism for the activation of the G-Furthermore, they suggest a mechanism for the activation of the G-protein. protein.
The screwing motion of helix 6 may provide a mechanical trigger for The screwing motion of helix 6 may provide a mechanical trigger for conformational changes in the G-protein which lead to GDP/GTP conformational changes in the G-protein which lead to GDP/GTP exchange.exchange.
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SPECIFIC AIM SPECIFIC AIM 11A.A. The mechanism of activation of Rhodopsin by improved The mechanism of activation of Rhodopsin by improved GNM/ANM AnalysisGNM/ANM Analysis
1. Side Chain Interactions 1. Side Chain Interactions
2. Water Molecules 2. Water Molecules
3. Chromophore3. Chromophore
4. Missing loops 4. Missing loops
5. Lipid molecules 5. Lipid molecules
B. Chromophore binding pocketB. Chromophore binding pocket
C. Surface exposure after opening of the helical bundle C. Surface exposure after opening of the helical bundle during activationduring activation
D. Rhodopsin oligomerizationD. Rhodopsin oligomerization
(Fotiadis et al, 2003 Nature)(Fotiadis et al, 2003 Nature)
E. Analysis of multiple modes of the GNM and ANME. Analysis of multiple modes of the GNM and ANM
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SPECIFIC AIM SPECIFIC AIM 22Interaction of rhodopsin with GInteraction of rhodopsin with Gtt
Analysis of GDP Analysis of GDP Bound form of Bound form of Heterotrimeric GHeterotrimeric Gt t by by GNM and ANM.GNM and ANM.
interaction surface for interaction surface for rhodopsin- Grhodopsin- Gtt complex by complex by electrostatic surface maps electrostatic surface maps of the active form of of the active form of rhodopsin found by ANM rhodopsin found by ANM analysis and Ganalysis and Gtt. .
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SPECIFIC AIM SPECIFIC AIM 33
by exploring the importance of hinge regions by by exploring the importance of hinge regions by sequence alignment. sequence alignment.
by applying the GNM and ANM analysis to other by applying the GNM and ANM analysis to other members of the family whose structures are members of the family whose structures are determined theoretically.determined theoretically.
Extension of the mechanism of Activation to other GPCRsExtension of the mechanism of Activation to other GPCRs
Adrenergic receptorsAdrenergic receptorsMetabotropic Glutamate receptorsMetabotropic Glutamate receptors
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Thanks toThanks toDr. Ivet BaharDr. Ivet Bahar Dr. Judith KleinDr. Judith Klein
-Seetharaman-Seetharaman
Post-Docs:Dr. Rajan MunshiDr. Dror Tobi
Dr. A.J. Rader
Graduate StudentsElife Zerrin BagciShann-Ching ChenChris Myers Alpay TemizLee-Wei Yang
Dr Mike Cascio Dr Billy DayDr Christine MilcarekDr Hagai MeirovitchDr Tom Smithgall
System AdministratorsDr. Rob Bell Mark Holliman AdministratorsJoseph BaharNancy Gehenio
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Summary and ConclusionSummary and ConclusionThe elastic network models (GNM and ANM) are tools to explore the dynamics of
proteins and to determine the critically important sites. These are classified in two categories:
The first category comprises the residues that are important for coordinating the cooperative motions of the overall molecule. These are identified from the minima of the global mode shapes.
Their mutation can impede function. The second one consists of residues experiencing an
extremely strong coupling to their close neighbors, and thereby undergoing the highest frequency/smallest amplitude vibrations.
Their mutation can impede stability. Both groups of residues are expected to be evolutionarily
conserved, the former for function requirements, and the latter for folding and stability
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Experiments to validate the calculationsExperiments to validate the calculations
Site directed spin labeling combined with EPRSite directed spin labeling combined with EPR
Cysteine scaning mutagenesis:-Reactivity and solvent accesibility of the sulfhydryl groups to 4-PDS. Absorbance of 4-TP at 323nm-Disulfide exchange of thiopyridinyl-derivatives of rhodopsin by sulfhydryl reagents (R-SH) both in dark and after illumination. -Disulfide bond formation in double cysteine mutation assay. Rate of cysteine bond formation is a measure of proximity of the mutant. In addition, sulfur bridges can inhibit light activation. This can show the necessary movements of helices to form MetaII. Site directed 19F labeling for NMR study: single cysteine mutants followed by the attachment of TET (CF3-CH2-S) attachment. Shifts in dark and light NMR spectra of the mutants shows the movements of residues.Antibody binding experiments: Antibodies which bind to Meta II but not rhodopsin in the dark.
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Experiments to validate the calculationsExperiments to validate the calculationsRhodopsin-Gt InteractionRhodopsin-Gt Interaction
Gt activation by fluoresence spectroscopy: After GTPS addition, increase in fluoresence results from exposure of Trp207 in Gt. when Gt is activated.
Assay of Meta II-Gt complex: Flash photolysis (light scattering). A flash induced light scattering increases over time in the presence of binding but not in the absence of Gt. This signal reflects the binding of Gt to R. In the presence of Gt and GTP, a flash produces a decrease of scattering intensity due to a loss of scattering mass.
Nucleotide release assay for GDP release ability. Samples are filtered through a nitrocellulose membrane. The amount of [32P]GDP is filtered and quantitated.
Peptide competition assays.