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Page 1: MCQs FORMina Mata disease related to [A] Methyl Mercury [B] Uranium [C] Methyl Cyanide [D] Lead. Agricultural Biotechnology 7 55. Which element constitute thin base of ... RAPD [D]
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MCQs FORM.Sc. BIOTECHNOLOGY

ENTRANCEEXAMINATIONS

Dr. Vishal P. Deshmukh Dr. Varsha V. DeshmukhAssistant Professor, CHB Lecturer,Department of Botany, Department of Botany,

Jagadamba Mahavidyalaya, Sant Gadge Baba Amravati University,Achalpur City, Dist. Amravati (Maharashtra). Amravati (Maharashtra).

Dr. Vivek R. NarkhedkarSenior Research Fellow,Department of Botany,

Sant Gadge Baba Amravati University,Amravati (Maharashtra).

EditorDr. Prashant V. Thakare

Associate Professor,Department of Biotechnology,

Sant Gadge Baba Amravati University,Amravati (Maharashtra).

ISO 9001:2015 CERTIFIED

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© AUTHORS

No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by anymeans, electronic, mechanical, photocopying, recording and/or otherwise without the prior written permission of theauthors and the publisher.

First Edition : 2019

Published by : Mrs. Meena Pandey for Himalaya Publishing House Pvt. Ltd.,“Ramdoot”, Dr. Bhalerao Marg, Girgaon, Mumbai - 400 004.Phone: 022-23860170, 23863863; Fax: 022-23877178E-mail: [email protected] Website: www.himpub.com

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DTP by : Sudhakar Shetty

Printed at : M/s. Aditya Offset Process (I) Pvt. Ltd., Hyderabad. On behalf of HPH.

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FOREWORD

Biotechnology is now an established discipline showing its own impacts in various facets of

human life. Since its emergence, students of Biotechnology played an important role in the

development of Biotechnology in India. To fulfill the needs of M.Sc. Biotechnology aspirant students,

the book MCQs for M.Sc. Biotechnology Entrance Examinations by Dr. Vishal P. Deshmukh,

Dr. Varsha V. Deshmukh, Dr. Vivek R. Narkhedkar and Dr. Prashant V. Thakare is a welcome effort.

The students preparing for M.Sc. Biotechnology needs to go through almost all branches of Life

Sciences. To cope up with this need, authors have devised their own questions covering most

important topics which is the beauty of this book. To provide wide experience of examination patterns,

latest solved question papers of top Indian Universities are also provided. Authors have substantial

experience in teaching and research as well writing books for competitive examinations.

I applaud Dr. Vishal P. Deshmukh, Dr. Varsha V. Deshmukh, Dr. Vivek R. Narkhedkar and

Dr. Prashant V. Thakare for bringing such innovative book. I am very sure that this book will be

widely accepted among students.

I extend my best wishes to authors for the success of this book.

Dr. M.K. Rai

Dr. Mahendra RaiBasic Science Research Faculty Fellow (UGC)Former Professor and Head, Department of Biotechnology,Sant Gadge Baba Amravati University, Amravati-444 602,Maharashtra, India.Email: [email protected]

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PREFACE

We are bringing out this book for the students who aspire to appear for M.Sc. Biotechnologyentrance examinations conducted by universities and institutions like IIT-JAM, JNU, TIFR, AIEEA-PG, CU-CET, BHU, NCBS and the top Universities across India.

Considering the diverse pattern of examinations conducted by the various Universities andInstitutions, we have prepared a comprehensive Question bank of more than 1200 MCQs with answers.We have also provided some entrance examination preparation strategies. These MCQs, distributed in12 chapters, in the Part A of book covering a wide spectrum of possible questions, most probably willbe asked in M.Sc. Biotechnology entrance examinations.

The most exciting, demarcating and vital part of the book is the Part B. This part contains latestyear question papers collected from the various top reputed Universities and Institutions of differentstates of India with their correct answer keys. This unique collection of question papers from differentUniversities of India will help the students to have great collection of divert MCQs. This will also helpthem to know the trends or pattern of questions asked in various Universities’ M.Sc. Biotechnologyentrance examinations.

Besides it will also help the students to know about various Universities offering this programmein different states of India, so that they can also apply for other Universities besides parent Universityonly. The book serves as a vital handbook for M.Sc. Biotechnology entrance examinations as far asobjective pattern is concerned. We feel satisfied if the book serves the purpose for which it is intended.

Our most sincere thanks to Dr. Durgesh Borse, Assistant Professor, Department of English,GVISH, Amravati for critically reviewing the book details and helping to improve the book content.

The first author is grateful to Shri Ramakant Sherkar, the President, Shri Jagadamba VinkarShikshan Sanstha, Sarmaspura, Achalpur and the Principal, Dr. P.G. Rohankar for their immensesupport and constant cooperation.

We must record our special appreciation to our families for their all kinds of support.We shall welcome any suggestions/criticisms for the further improvement of book since there

always remains a scope for the same.Lastly, we would like to express our appreciation for the support provided by Mr. Gokul Pandey

of Himalaya Publishing House Pvt. Ltd. and the production staff who strived hard to bring this book inits shape.

Dr. Vishal P. DeshmukhDr. Varsha V. DeshmukhDr. Vivek R. NarkhedkarDr. Prashant V. Thakare

8th October, 2019, Vijaya DashamiAmravati

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TABLE OF CONTENTS

PART - A

Sr. No. Name of Chapter Page No.

1 Agricultural Biotechnology 3-10

2 Basic Life Sciences (Botany, Zoology, Microbiology) 11-24

3 Biochemistry 25-32

4 Biotechnology 33-40

5 Cell Biology 41-48

6 Cytology and Genetics 49-57

7 Environmental Ecology 58-65

8 Immunology 66-76

9 Molecular Biology 77-85

10 Plant Physiology 86-96

11 Techniques in Life Sciences 97-106

12 Tissue Culture 107-116

PART - B

Sr.No. Year University Page No.

Andhra Pradesh

1 2017-18 The Dravidian University (Biotechnology, Biochemistry andMicrobiology) 119-128

2 2016-17 Acharya Nagarjuna University (Life Sciences) 129-1373 2016-17 Andhra University (Life Sciences) 138-1454 2016-17 Sri Krishnadevaraya University (Biotechnology) 146-154

Assam5 2017-18 Dibrugarh University (Biotechnology and Bioinformatics) 155-1626 2016-17 Dibrugarh University (Biotechnology and Bioinformatics) 163-171

Chhattisgarh7 2017-18 Pt. Ravishankar Shukla University (Biotechnology) 172-175

Delhi8 2017-18 Jamia Millia Islamia University (Biotechnology) 176-1869 2017-18 JAM IIT (Biological Sciences) 187-194

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10 2017-18 JAM IIT (Biotechnology) 195-20311 2016-17 JAM IIT (Biotechnology) 204-210

12 2016-17 Jawaharlal Nehru University Combined Entrance Examination(Biotechnology) 211-227

13 2016-17 Jawaharlal Nehru University Combined Entrance Examination(Agricultural Biotechnology) 228-242

14 2015-16 JAM IIT (Biotechnology) 243-250Goa15 2017-18 Goa University (Biotechnology) 251-256Haryana16 2013-14 Kurukshetra University (Biotechnology) 257-261Himachal Pradesh17 2016-17 Himachal Pradesh University (Biotechnology) 262-271Jammu and Kashmir18 2017-18 Shri Mata Vaishno Devi University (Biotechnology) 272-27619 2017-18 University of Kashmir (Biotechnology) 277-282Karnataka

20 2017-18 Joint Graduate Entrance Examination for Biology and InterdisciplinaryLife Sciences (JGEEBILS) 283-291

Kerala21 2018-19 Kannur University (Biotechnology) 292-300Madhya Pradesh22 2018-19 Dr. Hari Singh Gour University (Biotechnology) 301-31023 2017-18 Rani Durgawati University, Jabalpur (Biotechnology) 311-318Maharashtra24 2018-19 Shivaji University (Biotechnology/Biochemistry) 319-326Mizoram25 2018-19 Mizoram University (Biotechnology) 327-32926 2017-18 Mizoram University (Biotechnology) 330-332Odisha27 2018-19 Utkal University (Biotechnology) 333-33728 2018-19 Fakir Mohan University (Biotechnology) 338-34229 2017-18 Ravenshaw University (Biotechnology) 343-35130 2017-18 Utkal University (Biotechnology) 352-356Rajasthan31 2018-19 Central University of Rajasthan (Biotechnology) 357-365

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Tamil Nadu32 2017-18 Periyar University (Biotechnology) 366-369Telangana33 2016-17 Kakatiya University (Biotechnology) 370-37634 2016-17 The University of Hyderabad (Plant Biology and Biotechnology) 377-387Uttar Pradesh35 2017-18 Banaras Hindu University (Plant Biotechnology) 388-396Uttarakhand36 2017-18 Hemvati Nandan Bahuguna Garhwal University (Biotechnology) 397-403West Bengal37 2017-18 The University of Kalyani (Biotechnology) 404-40838 2016-17 The University of Burdwan (Biotechnology) 409-413

References 414

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PART - A

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2 MCQs for M.Sc. Biotechnology Entrance Examinations

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Agricultural Biotechnology 3

1. Ganga-1 is a[A] Maize hybrid[B] Cotton hybrid[C] Mutant wheat variety[D] Cotton androgenic haploid

2. Blue revolution in India is related to[A] Milk [B] Silk[C] Fish [D] Egg

3. Which is the source country forintroduction of IR-8 rice variety in India?[A] Japan [B] Australia[C] Cuba [D] Philippines

4. Yeast two-hybrid system is used for[A] DNA-protein interactions[B] Protein-protein interactions[C] C-C interactions[D] Protein-lipid interaction

5. Transposons are known as[A] Physical mutagen[B] Biological mutagen[C] Chemical mutagen[D] None of the above

6. High content Montmorillonite is found in[A] Costal line soil[B] Sandy loam

[C] Red soil[D] Black cotton soil

7. If two Restriction enzymes recognisingsame sequence, they are known as[A] Isomers [B] Epimers[C] Enantiomer [D] Isoschizomers

8 Which is the biological precursor ofVitamin D2?[A] Carotenoids [B] Tocopherol[C] Ergosterol [D] Riboflavin

9. Which method is suitable to detect virusinfection in seeds?[A] Tetrazoloin test[B] Triple response[C] ELISA[D] All of the above

10. For which Dee-gee-woo-gen gene isresponsible in rice?[A] Early maturity[B] Dwarfing[C] Virus resistance[D] High lysine

11. In vitro packaging is related to[A] Plasmid [B] YAC[C] -Phage [D] BAC

1 AGRICULTURALBIOTECHNOLOGY

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4 MCQs for M.Sc. Biotechnology Entrance Examinations

12. Toxicity of Hydrogen sulphide in ricecause[A] Bunchy top disease[B] Khaira disease[C] Blast disease[D] Akiochi disease

13. While isolating DNA from plant sources;the plasma membrane is ruptured by?[A] EDTA [B] CTAB[C] SDS [D] Both [B] and [C]

14. A vector which can be used for both,prokaryotes as well as eukaryotes isknown as[A] Shuttle vector[B] Expression vector[C] MAC[D] Plasmid

15. Who played vital role in making firstcloned animal “Dolly”?[A] S. Hatch [B] Wilmut[C] Sompal [D] Bryne

16. Which of the following is impartpungency test to chilli?[A] Anthocyanin[B] Capsaicin[C] Allyl propyl disulphide[D] None of the above

17. What is the ploidy status of commonbread wheat (2n = 42)?[A] Diploid [B] Monoploid[C] Hexaploid [D] Octaploid

18. Which of the following is an example ofSCP?[A] E. coli [B] Chara[C] Sargassum [D] Spirulina

19. A hybrid plasmid containing cos site oflambda phage is known as?

[A] pBR 322 [B] pUC[C] Cosmid [D] YAC

20. Which plant part is used to produce virusfree plantlets using tissue culturetechniques?[A] Seeds[B] Apical meristem[C] Phloem parenchyma[D] Petiole

21. The fruit type of Ber fruit is[A] Etaerio of follicle[B] Legume[C] Siliqua[D] Drupe

22. Golden rice is a source of[A] Fatty acids [B] Vit. A[C] Vit. E [D] Citric acid

23. Nagaheru U-triangle related to[A] Brassica [B] Maize[C] Wheat [D] Onion

24. Identify the step NOT involve inpreparing Genomic library?[A] Isolation [B] Amplification[C] Digestion [D] Transformation

25. The technique used for fractionation ofprotein according to their mass is knownas[A] SDS-PAGE [B] PCR[C] FISH [D] SNPs

26. Ringworm in cattle is caused by[A] Algae [B] Bacteria[C] Fungi [D] Viruses

27. Real-time PCR is also called as[A] qPCR[B] Nested PCR

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Agricultural Biotechnology 5

[C] Hot Start PCR[D] None of the above

28. Cross between wheat x rye is known as[A] Triticale[B] Raphanobrassica[C] Pomato[D] None of the above

29. Vitamin K is also known as[A] Antisterility vitamin[B] Coagulating vitamin[C] Vitamin B1[D] Vitamin B3

30. Cytoplasmic male sterility in plants isdue to factor positioned on[A] m RNA[B] Mitochondrial DNA[C] Genomic DNA[D] Chloroplast DNA

31. Identify three correct termination codons[A] AAU, UAG, UGA[B] UGA, UAA, UAG[C] UAG, UAA, UGC[D] UAA, CUG, UCG

32. Which of the following is related toorganic farming in India?[A] ICRISAT [B] CSIR[C] LEISA [D] NABARD

33. Teminism theory related to[A] Replication[B] Transcription[C] Reverse transcription[D] Termination

34. The first plant genome sequenced is of[A] Neurospora[B] Arabidopsis thaliana

[C] Rice[D] Wheat

35. A fluorescent dye used to visualise DNAon agarose gel electrophoresis under UVlight is[A] Coomassie brilliant blue[B] Bromophenol blue[C] Ethidium bromide[D] Light green

36. Sanager method of DNA sequencing isalso known as[A] Deoxy method[B] Dideoxy method[C] Mono deoxy method[D] HTS method

37. Which of the following is produced byfungi?[A] Antibodies [B] Aflatoxins[C] Interferons [D] Elaters

38. Which test is most suitable to estimatepopulation variance?[A] Chi square test[B] F-test[C] Mean[D] Z-test

39. Which is the most sensitive method fordetection of protein?[A] Northern blotting[B] Southern blotting[C] Western blotting[D] TLC

40. Synthetic seeds are prepare from[A] Hybrid seed[B] Somatic embryos[C] Root tips[D] Local seeds

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6 MCQs for M.Sc. Biotechnology Entrance Examinations

41. Norin-10 is a variety of[A] Pineapple [B] Rice[C] Wheat [D] Mango

42. Which is the longest phase in Mitosis?[A] Prophase [B] Anaphase[C] Metaphase [D] Telophase

43. Formation of Crown gall disease due toAgrobacterium infection in plants is dueto[A] Hyperplasia[B] Hypertrophy[C] Both [A] and [B][D] Cell cyclosis

44. The genetic material in M13bacteriophage is[A] Circular SS DNA[B] Linear SS DNA[C] Circular DS DNA[D] None of the above

45. Rumen is the largest part of[A] Non-ruminant stomach[B] Ruminant lung[C] Ruminant stomach[D] Ruminant rectum

46. Which of the following is component ofVitamin B12[A] K [B] Mo[C] Co [D] Zn

47. Which of the following test is used todetermine seed viability?[A] ELISA test[B] Tetrazoloin test[C] Sucrose test[D] None of the above

48. Who brought ‘Triticale’ by crossingbread wheat with rye?[A] Rimpu [B] Shull[C] Barrus [D] De Barry

49. Which of the following represents co-dominant marker?[A] RFLP [B] SSR[C] RAPD [D] Both [A] and [B]

50. Which of the following is commonlyknown as Mosquitofish?[A] Horse fish[B] B. bola[C] Gambusia affinis[D] Cyprinous carpio

51. From which part of Brassica napus,Brassinosteroids a plant hormone wasfirstly isolated?[A] Leaf [B] Pollen[C] Root [D] Root hairs

52. Vitamin E is also known as[A] Antisterility vitamin[B] Anti coagulation factor[C] Ascorbic acid[D] Riboflavin

53. In okra, the vector for “yellow veinmosaic” is[A] Wasps[B] White fly[C] Stem borer weevil[D] Mosquito

54. Mina Mata disease related to[A] Methyl Mercury[B] Uranium[C] Methyl Cyanide[D] Lead

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Agricultural Biotechnology 7

55. Which element constitute thin base ofDNA chip?[A] Gold [B] Silver[C] Silicon [D] Titanium

56. Vacuole membrane is known as[A] Nuclear membrane[B] Tonoplast[C] Middle lamella[D] Leucoplast

57. Which is the best method for bacterialstorage?[A] Antibiotic treatment[B] Lyophilisation[C] Heat treatment above 100ºC[D] UV treatment

58. Local varieties growing over a period oftime in any area are called as[A] Haustoria[B] Land races[C] R line[D] Improved varieties

59. BGA is most useful biofertiliser for[A] Rice [B] Wheat[C] Maize [D] Moringa

60. RFLP is a[A] Morphological marker[B] Biochemical marker[C] Genetic marker[D] Cytological marker

61. Who is known as Drosophila of plantkingdom?[A] Neurospora [B] E. coli[C] Yeast [D] Penicillium

62. The microorganism facilitate uptake ofimmobile phosphorus to plants is

[A] Spirulina [B] VAM[C] E. coli [D] Rhizobium

63. Identify the gaseous hormone[A] Auxin [B] Cytokinins[C] Ethylene [D] Zeatin

64. Which of the following is a dry Bt, usedfor a caterpillar control?[A] DDT [B] DiPel[C] Diazinon [D] Deltamethri

65. Which of the following is a DominantMarker?[A] AFLP [B] SSR[C] RAPD [D] Both [A] and [B]

66. What is the denaturation temperature forDouble stranded DNA?[A] 32ºC [B] 55ºC[C] 72ºC [D] 94ºC

67. 2n-1-1 chromosome compliment of anindividual represents[A] Nullisomy[B] Double trisomy[C] Double monosomy[D] Trisomy

68. Metaxenia is reported in[A] Mango [B] Potato[C] Date palm [D] Brinjal

69. Which of the following is responsible forstomata closing?[A] NaCl[B] IAA[C] Phenyl Mercuric acetate[D] Boron

70. “Gene for Gene” hypothesis was givenby[A] Flor [B] Davenport[C] Vavilov [D] Donald

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8 MCQs for M.Sc. Biotechnology Entrance Examinations

71. In maize, Opaque-2 mutation isassociated with[A] Decreased Lysine content[B] Increased Methionine content[C] Increased Glycine content[D] Increased Lysine content

72. While heating, which vitamin in milkremains heat resistant?[A] Vitamin K [B] Vitamin A[C] Vitamin E [D] Vitamin D

73. Naked RNA viruses are called as[A] Viroid [B] Virion[C] Prions [D] Virulence

74. Which of the following techniqueamplify DNA exponentially?[A] SDS-PAGE [B] NMR[C] PCR [D] Chromatography

75. Which of the following is ER residentchaperon?[A] HSP 100 [B] Bip protein[C] H4 [D] HSP 80

76. Which of the following permits the trialsof GM crops in India?[A] Prime Minister office[B] Ministry of Agriculture[C] Standing committee[D] GEAC

77. Which amino acid is a precursor ofhormone IAA?[A] Tryptophan[B] Farnesyl diphosphate[C] Niacin[D] Dopamine

78. Which of the following is an interspecificvariety of cotton?

[A] Pusa phalguni[B] Sangam[C] Varalaxmi[D] Prabhat

79. In India, Bunchy top disease is associatedwith[A] Apple [B] Maize[C] Banana [D] Canola

80. Gizzard is the largest body part of whichanimal[A] Pig [B] Cow[C] Horse [D] Poultry

81. The element involve in stomatalmovement is[A] Cu [B] I[C] Mo [D] K

82. The marker used to develop high densitymolecular map is[A] SCAR [B] SDS-PAGE[C] RAPD [D] SNPs

83. Causative agent for “Mad Cow” diseaseis[A] Prions[B] RNA[C] DNA[D] Both DNA and RNA

84. National research centre for Equines islocated at?[A] Palampur [B] Hisar[C] Uttrarkashi [D] Bikaner

85. Which of the following techniqueproduces haploid in Barley?[A] Callus culture[B] Bulbosum technique[C] Apical meristem culture[D] Ovule culture

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Agricultural Biotechnology 9

86. Which of the following is stress relatedamino acid?[A] Valine[B] Tryptophan[C] Glutamic acid[D] Proline

87. “Flavr Savr” a GMO related to[A] Potato [B] Tomato[C] Grape [D] Spinach

88. “Prussic acid poisoning” or “Sorghumpoisoning” is due to[A] HCN [B] HCL[C] KCl [D] H2SO4

89. In plants, stable transformation of gene isachieved through?[A] Agrobacterium[B] Gun method[C] Electroporation[D] PEG

90. The precursor of hormone ethylene is[A] Ascorbic acid[B] Methionine[C] Tryptophan[D] Ubiquitin

91. What is the temperature of liquidNitrogen?[A] –196ºC [B] –90ºC[C] –20ºC [D] 0ºC

92. Which of the following represents thefirst plant to undergo transgenic activity?[A] Tomato [B] Cotton[C] Brassica [D] Tobacco

93. Edible portion of Mango is[A] Epicarp [B] Mesocarp[C] Pericarp [D] Fleshy thalamus

94. Which of the following plants is usefulfor phytoremidiation?[A] Date palm [B] Grapes[C] Carrot [D] Apple

95. Bioluminescence is generated by[A] Luciferin [B] Acetylene[C] Biotin [D] Pectin

96. Which of the following is a source ofBio-diesel?[A] Brassica [B] Sun hemp[C] Nostoc [D] Jatropha

97. DNA fingerprinting involves[A] HPTLC [B] SDS-PAGE[C] DOT BLOT [D] RFLP

98. Who brought “White revolution” in India?[A] Swaminathan [B] Kurien[C] Datta [D] Borlaug

99. Cow milk is rich in[A] Casein [B] Starch[C] Albumin [D] All of the above

100. While preparing cDNA library one mustisolate[A] tRNA [B] gRNA[C] rRNA [D] mRNA

101. What is the chromosome number ofTriticum aestivum L. (2n = 6x = 4z)endosperm cells?[A] 63 [B] 21[C] 7 [D] 42

Answers:1. [A], 2. [C], 3. [D], 4. [B], 5. [B], 6. [D] 7. [D],8. [C], 9. [C] 10. [B], 11. [C], 12. [D], 13. [D],14. [A], 15. [B], 16. [B], 17. [C], 18. [D], 19. [C],20. [B], 21. [B], 22. [D], 23. [B], 24. [A], 25. [A],26. [C], 27. [A], 28. [A], 29. [B], 30. [B], 31. [B],32. [C], 33. [C], 34. [B], 35. [C], 36. [B], 37. [B],

Page 23: MCQs FORMina Mata disease related to [A] Methyl Mercury [B] Uranium [C] Methyl Cyanide [D] Lead. Agricultural Biotechnology 7 55. Which element constitute thin base of ... RAPD [D]

10 MCQs for M.Sc. Biotechnology Entrance Examinations

38. [B], 39. [C], 40 [B], 41. [C], 42. [A], 43. [C],44. [A], 45. [C], 46. [C], 47. [B], 48. [A], 49. [D],50. [C], 51. [B], 52. [A], 53. [B], 54. [A], 55. [C],56. [B], 57. [B], 58. [B], 59. [A], 60. [C], 61. [A],62. [B], 63. [C], 64. [B], 65. [C], 66. [D], 67. [C],68. [C], 69. [C], 70. [A], 71. [D], 72. [B], 73. [A],74. [C], 75. [B], 76. [D], 77. [A], 78. [C],

79. [C], 80. [D], 81. [D], 82. [D], 83. [A],84. [B], 85. [B], 86. [D], 87. [B], 88. [A], 89. [A],90. [B], 91. [A], 92. [D], 93. [B], 94. [C], 95. [A],96. [D], 97. [D], 98. [B], 99. [A], 100. [D],101. [A]