maternal secretor status and child microbiota compositionsecretor status unweighted unifrac weighted...
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Maternal Secretor Status and Child
Microbiota Composition
Paula Smith-Brown Paediatric Dietitian & PhD Scholar
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© Copyright on the content of this presentation is reserved to the Children’s Nutrition Research Centre, The University of Queensland.
The First 1000 Days
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Development of Microbiota
Yatsunenko et al. Nature 000, 1-7 (2012)
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WHO, Infant Feeding
Recommendations, 2001
Infants should be exclusively breastfed
for the first six months of life.
Thereafter, infants should receive
nutritionally adequate and safe
complementary foods while
breastfeeding continues
for up to 2 years of age or beyond.
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Breast Milk Composition
Angela M. Zivkovic et al. PNAS 2011;108:4653-4658
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Human Milk Oligosaccharides
(HMOs)
Castanys-Muňoz et al. Nutr Rev, 2013; 71(12):773 -89
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Lewis Blood Group and HMOs
Bode & Jantscher-Krenn. Adv Nutr 2012; 3(3):383S-91S
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Prevalence of Non-Secretor Status
Ferrer-Admetlla et al. Mol Biol Evol 2009; 26(9): 1993-2003
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Microbiota of Breastfed Infants by
Maternal Secretor Status.
Lewis et al.
Microbiome 2015; 13
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Study Design
Recruit
• Feeding Queensland Babies Study Cohort
• 37 children (2 -3 yrs) + 17 mothers
Secretor Status
• Blood and saliva samples
• Hemagglutination Inhibition Technique
Microbiota
• Faecal samples
• Illumina sequencing of rRNA gene (V6-8)
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UniFrac Distance Metric
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Microbiota Composition by
Secretor Status
Unweighted UniFrac Weighted UniFrac
Sample size (secretor)
R2 p R2 p
Child Secretor Status 28 (20 S) 0.069 0.030 0.023 0.699
Mother’s Secretor Status 17 (11 S) 0.071 0.256 0.104 0.111
Mother’s Secretor Status - ABF 14(10 S) 0.111 0.102 0.116 0.138
Mother’s Secretor Status – EBF 11 (8 S) 0.167 0.028 0.159 0.081
Smith-Brown et al, PLOS One, 19 Sept 2016
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Prevotella Abundance
by Secretor Status
Median Prevotella Abundance by Secretor Status
Sample size (secretor)
p pFDR S N-S
Child Secretor Status 28 (20 S) < 0.001 <0.001 0 3
Mother’s Secretor Status 17 (11 S) < 0.001 <0.001 0 4
Mother’s Secretor Status – ABF 14(10 S) < 0.001 <0.001 0 4055.5
Mother’s Secretor Status – EBF 11 (8 S) < 0.001 <0.001 0 8106
Smith-Brown et al, PLOS One, 19 Sept 2016
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Genus Mean Abundance
by Maternal Secretor Status
Smith-Brown et al, PLOS One, 19 Sept 2016
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PICRUSt PREDICTED METAGENOME
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KEGG Functional Pathway Abundance
Child Secretor Status
Smith-Brown et al, PLOS One, 19 Sept 2016
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KEGG Functional Pathway Abundance
Mother Secretor Status
Smith-Brown et al, PLOS One, 19 Sept 2016
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KEGG Functional Pathway Abundance
Mother Secretor Status - EBF
Smith-Brown et al, PLOS One, 19 Sept 2016
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Conclusion
Non-secretor mother
No α1,2 HMO
↓ Bifidobacteria
↑ acidic HMO
↑ Prevotella
Non-secretor child
No α1,2 glycans
↑Arachidonic Acid Metabolism
↑ Inflammation
↑Prevotella
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Acknowledgements
• Supervisors:
– Professor Peter Davies – Children’s Nutrition
Research Centre, University of Queensland
– Professor Mark Morrison and Associate
Professor Lutz Krause, The University of
Queensland Translational Research Institute.
• Feeding Queensland Babies Study:
– Dr Ruth Newby, University of Sunshine Coast
• Funding: Danone Nutricia Australia
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References
1 Yatsunenko, T. et al. Human gut microbiome viewed across age and geography. Nature 486,
222-227, doi:10.1038/nature11053 (2012).
2 Zivkovic, A. M., German, J. B., Lebrilla, C. B. & Mills, D. A. Human milk glycobiome and its
impact on the infant gastrointestinal microbiota. Proceedings of the National Academy of
Sciences 108, 4653-4658, doi:10.1073/pnas.1000083107 (2011).
3 Castanys-Munoz, E., Martin, M. J. & Prieto, P. A. 2'-fucosyllactose: an abundant, genetically
determined soluble glycan present in human milk. Nutr Rev 71, 773-789,
doi:10.1111/nure.12079 (2013).
4 Bode, L. & Jantscher-Krenn, E. Structure-function relationships of human milk oligosaccharides.
Adv Nutr 3, 383s-391s, doi:10.3945/an.111.001404 (2012).
5 Ferrer-Admetlla, A. et al. A natural history of FUT2 polymorphism in humans. Molecular biology
and evolution 26, 1993-2003, doi:10.1093/molbev/msp108 (2009).
6 Lewis, Z. T. et al. Maternal fucosyltransferase 2 status affects the gut bifidobacterial
communities of breastfed infants. Microbiome 3, 13, doi:10.1186/s40168-015-0071-z (2015).