materials iaa solution, 0.01% w/v = 100 parts per million (ppm) radish seeds 8 test tubes or bottles...
TRANSCRIPT
Materials
• IAA solution, 0.01% w/v = 100 parts per million (ppm)
• Radish seeds
• 8 Test tubes or Bottles
• Distilled Water
• 8 Petri Dishes
• 8 Acetate Grids and Cotton wool
ACTIVITY
To investigate the effect of IAA growth regulator on plant tissue
Method
A. Preparing different concentrations of IAA
1. Label the 8 Petri dishes, droppers and bottles 1, 2, 3 . . . 8
2. Using a syringe, add 10 ml IAA solution to Bottle 1.
3. Using another syringe, add 9 ml distilled water to each of the bottles numbered 2 to 8.
7654321 8
4. Using Dropper 1, remove 1 ml IAA solution from Bottle 1 and add it to Bottle 2.
Place the cap on Bottle 2 and mix.
5. Using Dropper 2, remove 1 ml IAA solution from Bottle 2 and add it to Bottle 3. Mix.
6. Using a different dropper each time, repeat this procedure up to Bottle 7.
7. After mixing Bottle 7, remove 1 ml so that each bottle now contains 9 ml of solution.
8. Leave Bottle 8 as it is, with just 9 ml distilled water – it will act as the control.
Tube 1 2 3 4 5 6 7 8
IAA10 mls --
control
Water -- 9 mls 9 mls 9 mls 9 mls 9 mls 9 mls 9 mls
IAA conc
102 101 100 10-1 10-2 10-3 10-4 0
*ppm 100 10 1 0.1 0.01 0.001 0.0001 0
Serial dilution—making different concentrations of IAA
*ppm = parts per million
7. Draw a graph of the % stimulation and inhibition of root and shoot growth against IAA concentration.
Put IAA concentration on the horizontal [X] axis