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    INSTRUMENTS

    1.) UV Transilluminator .

    2 .) Incubator

    3) DI IT!" #!"!N$E

    %) &'T ("!TE

    ) Electro*+oretic c+amber

    6.) Micropipette

    Other material used:

    Petri plates

    Cotton plug

    Distilled water

    Conical flask

    2

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    Ligation Of DN :

    Agrose

    T 4 DNA ligaseλ

    / EcoRI digest

    2x ligase

    1% Agrose gel

    Loading Buffer

    1xTAE Buffer

    !lectrophoresis :

    Bromo !enol

    Agrose

    ETBr

    Plasmid DN "solation:

    L"sogen" Brot!L"sis solution

    Nutrilising #olution

    Et!anol $ stored at 2 &' (

    Tris Buffer

    DN "solation #rom Plant:

    L"sis Buffer

    )iEL*TE coloum

    +as! #olution

    Elution Buffer

    3

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    DN #rom !. Coli :

    TE Buffer

    L"sis Buffer

    ,!"nol - '!loroform $1-1(

    #odium acetate

    Iso !enol

    Et!anol

    $istriction and Digation :

    EcoR I

    )ind III

    2x Assa" Buffer

    Agarose .el

    Lamda Digest I

    $eplica Plating :

    #oil sam le

    Nutrient Agar

    Nutrient Brot!

    Iodine solution

    %

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