material required 1111
TRANSCRIPT
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8/18/2019 Material Required 1111
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Materials required
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Materials required
INSTRUMENTS
1.) UV Transilluminator .
2 .) Incubator
3) DI IT!" #!"!N$E
%) &'T ("!TE
) Electro*+oretic c+amber
6.) Micropipette
Other material used:
Petri plates
Cotton plug
Distilled water
Conical flask
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Materials required
Ligation Of DN :
Agrose
T 4 DNA ligaseλ
/ EcoRI digest
2x ligase
1% Agrose gel
Loading Buffer
1xTAE Buffer
!lectrophoresis :
Bromo !enol
Agrose
ETBr
Plasmid DN "solation:
L"sogen" Brot!L"sis solution
Nutrilising #olution
Et!anol $ stored at 2 &' (
Tris Buffer
DN "solation #rom Plant:
L"sis Buffer
)iEL*TE coloum
+as! #olution
Elution Buffer
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Materials required
DN #rom !. Coli :
TE Buffer
L"sis Buffer
,!"nol - '!loroform $1-1(
#odium acetate
Iso !enol
Et!anol
$istriction and Digation :
EcoR I
)ind III
2x Assa" Buffer
Agarose .el
Lamda Digest I
$eplica Plating :
#oil sam le
Nutrient Agar
Nutrient Brot!
Iodine solution
%
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