For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2015 BD

BD Stem Cell ResourceMarkers of Self-Renewal and Differentiation

23-9953-02

Ectoderm Markers Embryonic Stem Cells Mesoderm Markers Stem Cell Relevant SignalingAstrocytesA2B5CD44FGFR3

GFAPRan-2S100B

Embryonic Stem Cell MarkersAlkaline PhosphataseCD9CD15 (SSEA-1, Lewis X)CD24CD29 (Integrin β1)CD30CD49f (Integrin α6)CD133 (AC133, Prominin-1)CD194 (CCR4)CD324 (E-Cadherin)CD326 (EpCAM)CD338 (ABCG2)c-MycCriptoDPPA4DPPA5 (ESG1)FGF-4FoxD3Frizzled-5GCNF (NR6A1)GCTM-2GDF-3Hsp27Lefty-ALin-28

Nanog (Human)Nanog (Mouse)NucleosteminOct3/4Oct3/4APodocalyxinPodocalyxin Associated Keratin SulfatePRC2 (Suz12)Rex-1RoninSFRP2Smad2/3Sox2SSEA-3SSEA-4SSEA-5STAT3STAT3 (pS727)STAT3 (pY705)STAT3-interacting protein 1TRA-1-60 AntigenTRA-1-81 AntigenUTF-1Zic3

Early Mesoderm MarkersBMP2BMP4BrachyuryCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD325 (N-Cadherin)

CrypticMIXL1NF-YANodalSca-1 (Ly6A/E)Snail

Erk-MAPK Signalingbasic FGFC-RafERKERK1ERK1/2 (pT202/pY204)ERK2ERK3

Mek1Mek1 (pS298)Mek1/2 (pS222)Mek2p38 MAPK (PT180/pY182)Ras

TGF Beta SignalingBMPR-IICD119 (IFN-γ receptor α-chain)c-MycDP-1E2F-1E2F-2E2F-3Id1Id2

Id3p16 INK4p107p300PP2A Catalytic αROCK-IROCK-IISmad2/3SP1

Wnt Signaling14-3-3α-Cateninβ-Cateninδ-Cateninγ-CateninAktCaM Kinase IICasein Kinase I εCasein Kinase II αCasein Kinase II βCBP (CREB-Binding Protein)

GSK-3βGSK-3β (pY216)HDAC3Jagged1Lgr5Neurogenin 3NicastrinNotch1PP2A Catalytic αSNAI2 (Slug)Wnt16

Jak-STAT SignalingCD130 (gp130)STAT1 (C-TERMINUS)STAT1 (pY701)STAT3STAT3 (pS727)STAT3 (pY705)

STAT4STAT4 (pY693)STAT5 (MGF)STAT5 (MGF) (PY694)STAT6STAT6 (pY641)

Notch SignalingCBP (CREB-Binding Protein)Jagged1Jagged2Neurogenin 3Nicastrin

Notch1Notch2Notch3Notch4

Heart - Cardiogenesis Markersα-ActininAnnexin VICardiac specific myosinCardiac Troponin ICardiac Troponin TCaveolin-2Caveolin-3CD13CD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD66CD66cCD106 (VCAM-1)CD117 (SCF R, c-kit)CD140a (PDGFRα)CD144 (VE-cadherin)CD166 (ALCAM)CD172a (SIRPA)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)

CD309 (VEGFR2, KDR)CD325 (N-Cadherin)Connexin-43DesminFlk-1 (KDR, VEGF-R2, Ly-73)GATA4GATA6HAND1HAND2Islet 1M-CadherinMLC2aMLC2vMyogeninMyosin Heavy ChainNF-YANkx2.5P-glycoprotein (MDR)SSEA-1Vimentin

Hemangioblast MarkersACEBrachuryCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD133 (AC133, Prominin-1)CD144 (VE-cadherin)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)

CD324 (E-Cadherin)EphB4Flk-1 (KDR, VEGF-R2, Ly-73)GATA1LMO-2PodocalyxinRunx1 (CBFA2)Tal (SCL)

Hematopoietic Stem Cell MarkersNegative Markers

CD2CD3 CD4CD7CD8CD10CD11bCD13CD14CD15 (SSEA-1, Lewis X)CD15s (Sialyl Lewis x)CD16CD19CD20

CD24CD33CD36CD38CD45 (Leukocyte Common

Antigen, Ly-5)CD45R (B220)CD48CD56 (NCAM)CD97CD114 (G-CSF Receptor)CD138 (Syndecan-1) CD235aGATA3

Mesenchymal Stem Cell Differentiation MarkersChondrocyte (Cartilage) Markers

AggrecanCD44CD151Collagen II

Matrillin-4OsteonectinSox9

Mesenchymal Stem Cell Markers (Fat)Negative Markers

CD11b (Integrin αM chain)CD14CD19CD45 (Leukocyte Common

Antigen, Ly-5)

CD79aCD117 (SCF R, c-kit)HLA-DR

Positive Markers

CD9CD10CD13CD29 (Integrin β1)CD44CD49d (Integrin α4)CD49e (Integrin α5)CD54 (ICAM-1)CD55CD59

CD73 (Ecto-5’-nucleotidase)CD90 (Thy-1)CD105 (Endoglin)CD106 (VCAM-1)CD146 (MCAM, MUC18)CD166 (ALCAM)FibronectinHLA IVimentin

Skeletal Muscle Markersα-ActininActinB-Enolase (ENO-3)CD146 (MCAM, MUC18)CD325 (N-Cadherin)DesminM-Cadherin

Myf5MyoDMyogeninMyosin heavy chainMyosin light chainTitin

Smooth Muscle MarkersCalmodulinDesmin

Smooth muscle myosinVimentin

Mesenchymal Stem Cell Markers (Bone Marrow)Negative Markers

CD11a (Integrin αL chain)CD11b (Integrin αM chain)CD14CD19CD31 (PECAM1)

CD34 (Mucosialin, gp 105-120) CD45 (Leukocyte Common

Antigen, Ly-5)CD79aHLA-DR

Positive Markers

CD10CD13CD14CD15 (SSEA-1, Lewis X)CD29 (Integrin β1)CD44 (Pgp-1, H-CAM)CD49d (Integrin α4)CD49e (Integrin α5)CD51 (Integrin αV)CD54 (ICAM-1)CD56 (NCAM)CD71 (Transferrin Receptor)CD73 (Ecto-5’-nucleotidase)CD90 (Thy-1)CD105 (Endoglin)CD106 (VCAM-1)

CD117 (SCF R, c-kit)CD120a (TNF Receptor Type I)CD120b (TNF Receptor Type II)CD146CD166CD166 (ALCAM)CD271 (p75, NGFR/NTR)Flk-1 (KDR, VEGF-R2, Ly-73)GD2Ly-6A/E (Sca-1)NucleosteminSSEA-4STRO-1TAZVimentin

Myoblast (Muscle) Markers

AcetylcholinesteraseActinB-Enolase (ENO-3)CD146 (MCAM, MUC18)CD325 (N-Cadherin)Desmin

Lactate Dehydrogenase (LDH)Myf-5MyoDPax3Pax7PK-M

Osteoblast (Bone) Markers

Alkaline PhosphataseCollagen 1Fibronectin

IGFBP-3OsteocalcinRUNX2

Positive Markers

BMI-1CaM Kinase IVCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD40f (Integrin α6 chain)CD44CD59CD90 (Thy-1)CD90.1 (Thy-1.1)CD105 (Endoglin)CD106 (VCAM-1)CD117 (SCF R, c-kit)CD133 (AC133, Prominin-1)CD164CD184 (CXCR4)CD201 (EPCR)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)CD325 (N-Cadherin)

CDw93 (C1qRp)CDw338 (ABCG2)DAZLFlk-1 (KDR, VEGF-R2, Ly-73)FOXO1G-CSF Gfi-1HOXB4Jak2Lmo2Ly-6A/E (Sca-1)MRP1NF-YANotch1P-glycoproteinRunx1 (CBFA2)Sox7Tal (SCL)WASP (Wiskott-Aldrich

Syndrome Protein)

Endothelial Cell MarkersCD14CD15 (SSEA-1, Lewis X)CD18 (Integrin β2 chain, CR3/CR4) CD29 (Integrin β1)CD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD36CD45 (Leukocyte Common

Antigen, Ly-5)CD45R (B220)CD49d (Integrin α4)CD49e (Integrin α5)CD49f (Integrin α6)CD54 (ICAM-1)CD56 (NCAM)CD62E (E-Selectin)CD62L (L-Selectin)CD62P (P-Selectin)CD102CD105 (Endoglin)CD106 (VCAM-1)CD109CD112CD116 (GM-CSF Receptor)CD117 (SCF R, c-kit)CD120a (TNF Receptor Type I)CD120b (TNF Receptor Type II)CD121a (IL-1 Receptor, Type I/p80)CD124 (IL-4 Receptor α)CD141 (Thrombomodulin)CD144 (VE-cadherin)CD146 (MCAM, MUC18)CD147 (Neurothelin)

CD151CD152 (CTLA-4)CD157CD166 (ALCAM)CD184 (CXCR4)CD192 (CCR2)CD201 (EPCR)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)CD209CD209a (CIRE, DC-SIGN)CD213a1CD213a2CD249CD252 (OX-40 Ligand)CD253 (TRAIL) CD262 (TRAIL-R2, DR5)CD322CD325 (N-Cadherin)CDw93 (C1qRp)CDw145Flk-1 (KDR, VEGF-R2, Ly-73)HIF-1αIP-10Ly-6A/E (Sca-1)NestinSTAT3STAT3 (pS727)STAT3 (pY705)STAT3-interacting protein 1Vimentin

Endoderm MarkersDefinitive Endoderm MarkersB-CateninCD184 (CXCR4)CD325 (N-Cadherin)GATA4

HNF-1β (TCF-2)HNF-3β (FoxA2)Sox17

Hepatic Endoderm Markersα-FetoproteinAlbuminASGPR 1CD26CD29 (Integrin β1)CD44H (Pgp-1, H-CAM)CD49f (Integrin α6)

CD90 (Thy-1)CD326 (EpCAM)GATA4HNF-1αHNF-1β (TCF-2)LDLRTat-SF1

Intestinal Stem Cell MarkersBMI-1EphB2

Lgr5Sox9

Pancreatic Endoderm MarkersCD49f (Integrin α6)CD142Chromogranin AGad65GlucagonGlut2InsulinIslet-1Islet-2NeuroD1

Neurogenin 3Neuropilin-2Nkx2.2Nkx6.1Pancreatic polypeptidePax-6PDX-1PTF1ASomatostatinSynaptophysin

Primitive Gut Tube MarkersCDX2 HNF-1β (TCF-2)

Induced Pluripotency Markersc-MycKLF4Lin-28

NanogOct3/4Sox2

Primordial Germ Cell MarkersBlimp-1CD15 (SSEA-1, Lewis X)CD117 (SCF R, c-kit)DAZLFABP9Fragilis

NanosOct3/4Piwi/HiwiSteelStella (Dppa3)VASA (DDX4)

Trophoblast MarkersCDX2Cytokeratin 7

Sox7Trop-2

Early Ectoderm MarkersNestinNotch1

TP63

Neural Crest Cell MarkersCD49d (Integrin α4)CD57 (HNK-1)CD271 (p75, NGFR/NTR)

MASH1Neurogenin 3Notch1

Visual SystemCD68CD325 (N-Cadherin)

Pax-6Recoverin

Neural Stem Cell MarkersA2B5BMI-1CD15 (SSEA-1, Lewis X)CD15s (Sialyl Lewis x)CD24CD29 (Integrin β1)CD49f (Integrin α6)CD54 (ICAM-1)CD81 (TAPA-1)CD95 (Fas/APO-1)CD133 (AC133, Prominin-1)CD146 (MCAM, MUC18)CD184 (CXCR4)CD271 (p75, NGFR/NTR)CD338 (ABCG2)

CD349 (Frizzled-9)Ki-67MSI1 (Musashi-1)MSl2 (Musashi-2)Nanog (Mouse)NestinNogginNotch1Pax-6Prominin-2PSA-NCAMSox1Sox2Vimentin

Neuronal Markersα-Synucleinα-Synuclein (pY125)β-Tubulin (Tuj1)CD24CD56 (NCAM)CD81 (TAPA-1)CD90 (Thy-1)CD90.1 (Thy-1.1)CD90.2 (Thy-1.2)CD112CD271 (p75, NGFR/NTR)CD325 (N-Cadherin)ChATContactinDoublecortinGABA B Receptor Gad65GAP-43 (Neuromodulin)GluR δ 2GluR2GluR5/6/7Glutamine SynthetaseHNF-3β (FoxA2)Islet-1Jagged1MAP2 (a+b)

MAP2BMASH1mGluR1mGluR1αNestinNeuroD1Neurofilament NF-MNeuron-restricted progenitorsNeuropilin-2Nicastrinp150 GluedPax-5P-glycoproteinPSD-95Serotonin Receptor 5-HT 2ARSerotonin Receptor 5-HT 2BRSMNSynapsin ISynaptophysinSynaptotagminSyntaxinTauTrkBTubbyTyrosine HydroxylaseVimentin

Oligodendrocyte MarkersCD44CD44H (Pgp-1, H-CAM)CD140a (PDGFR α)Galactocerebroside CMBP

O1O4Olig1Olig2RIP

Skin Precursor Cell MarkersCRABP2FibronectinNestin

Sca-1 (Ly6A/E)Vimentin

White colored markers are available from BD Biosciences

bdbiosciences.com/stemcell

LIFR

Jak

Stat3C-myc Sox2 Oct4 Nanog Smads 2/3

Smads 175 MEK/ERK

p pp

β-catenin

WntBMP4 bFGF

TGF-β/Activin

LIF

JAK-STAT

Self Renewal

Wnt BMP-SMad Erk-MAPKTGF-β/Activin

gp130

LiverSelf Renewing Pathway

Heart Skeletal Muscle Kidney Smooth Muscle

Endothelium

Hemangioblast

Proerythroblast

MegakaryocyteMyeloid Dendrtitic CellMonocyte

Monoblast Megakaryoblast

Skin and Hair

Glial Restricted Progenitor

Type 1 Astrocyte

Type 2 Astrocyte

Neuron

OligodendrocyteProgenitor

Oligodendrocyte

NeuronalRestrictedProgenitor

Neural CrestStem Cell

Neurons, GliaSmooth MuscleCardiac TissueChondrocytes

Osteocytes

MacrophageThrombocytes

(Platelets)

Myeloblast

Neutrophil BasophilEosinophil

NeutrophilicMyelocyte

BasophilicMyelocyte

EosinophilicMyelocyte

NeutrophilicBand Cell

BasophilicBand Cell

EosinophilicBand Cell

Erythroblast Progranulocyte

Normoblast

Reticulocyte

Erythrocyte(Red Blood Cell)

Egg

Primordial Germ Cell

Sperm

Osteoblast (Bone)

Adipocyte (Fat)Fibroblast

Myotube (Muscle) Plasmacytoid

Dendritic CellCommitted Lymphoid Progenitor

Pre B CellPre-NK Cell

NK Lymphoblast

NK Cell

B-Lymphoblast

B Cell

Plasma Cell

T Cell

T-Lymphoblast

Thymocyte

MyoblastChondrocyte (Cartilage)

Thymus Pancreas Thyroid Lung Intestine

Endoderm

Mesenchymal Stem Cell

Hematopoietic Stem Cell

Mesoderm

Neural Stem Cell Ectoderm Embryonic Stem Cell or Induced Pluripotent

Stem Cell